CAMK2A (NM_015981) Human Untagged Clone

CAT#: SC111598

CAMK2A (untagged)-Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2A
• Synonyms: CAMKA; CaMKIINalpha; MRD53; MRT63
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_015981 edited
  ATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
  AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
  GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
  GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
  GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
  ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
  GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
  CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
  ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
  TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
  GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
  CTGTACCAGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
  ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
  ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
  ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
  GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
  AACAAGAAGAGCGATGGTGTGAAGGAATCCTCAGAGAGCACCAACACCACCATCGAGGAT
  GAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCC
  ATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGACCCTGGCATGACAGCCTTC
  GAACCTGAGGCCCTGGGGAACCTGGTTGAGGGCCTGGACTTCCATCGATTCTATTTTGAA
  AACCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAATCCCCACATCCAC
  CTGATGGGCGACGAGTCAGCCTGCATCGCCTACATCCGCATCACGCAGTACCTGGACGCT
  GGCGGCATCCCACGCACCGCCCAGTCGGAGGAGACCCGTGTCTGGCACCGCCGGGACGGC
  AAATGGCAGATCGTCCACTTCCACAGATCTGGGGCGCCCTCCGTCCTGCCCCACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015981 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATACTGCACGAGGCGGTTCTCTGTTTG
  CACTCGGCAGCACGGGCAGGCAAGTGGTCCCTAGGTTCGGGAGCAGAGCAGCAGCGCCTC
  AGTCCTGGTCCCCCAGTCCCAAGCCTCACCTGCCTGCCCAGCGCCAGGATGGCCACCATC
  ACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGCAAGGGAGCCTTC
  TCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCTGCCAAGATCATC
  AACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAAGCCCGCATCTGC
  CGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCAGAGGAGGGACAC
  CACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGATATCGTGGCCCGG
  GAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTGGAGGCTGTGCTG
  CACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAATCTGTTGCTGGCC
  TCCAAGCTCAATGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGCCATACAGGTGGAGG
  GGGAGCAACAAGCATGGTTTGCGTTTGTAGGGACTTCTGGATATCTCTCCCCATAAGTGC
  TGCCGCAAGACCCCGCCCGAAAGCCTGGGGACCTGTGGGCCTGTGGGTCATCCTGTACAT
  CCTGCTGGGTGGGCACCCCCCGGTCTTGGAAGAGGACCAACACCGCTGTACCAGACAACA
  AAGCGGCCCCTAGAATTCCCATCCCGAATGGGCACTGCCCCCCGGAGCCAAGATTGGTAA
  TAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015981 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTCAACATACTTACTGCGT
  ATAAAGTCATGCAAAGAAAACAGTGCAGACAGTAGATCCTAGTGGATGTGCCAAGGTATT
  CCACTCAGAGTCAATCCCAGGGAAAGAGGGAAAGAGGAAAAGAAAGAGAGAATGCGAACC
  CGAGGCTGCAGGATGAGGCATGAAGAGTAGAAATTCCCAGTGCTTTGCTGTGGTCATCAG
  ACGCCAAGGGGAGAGAGGCAATGAAGACACACGCTCACGGGCCCCCCAAAAGTGGGTGGG
  GGGTGCTGGGGGCGGCACACAGATATGGTGAGATGAAATCCTGGGAAGTTCGGTAGAGAA
  GACCCCAGTTTGCGAGGGAAGCAAAGAAAAGTCCAGGTATTTCCATCCTGACAGCCTCCC
  TCCTCCTCTCTGCCCTGACTTGCTGTTCCTGAGTCAGTGCTGGCATCTGATGCTTCCACA
  GTCCCAAAAGGAACGCCTGTGTCAGCTCACCTTGGGACCGAAATGGCAGAGAGGCCCTTC
  TCCCCGGAGCTGAGTCCGCCTTGGCCCCAATAACCACAGGTGACAAGGTCCACCTCAGAG
  ATGACAAAAAAAAAAAAAAAAACTAGAACAGAAAAAAACTACCCCTCACCCCTCTTCCTA
  CACCCCTTCCAACCTGACCCTTCTCACAATAATCTGAGAAGTCTAGTTATTACATAAATA
  TCCATTAACGCGAAATCCATTTACGAAATACACAGAAATTTGGCTATAAAAAGCATGCGG
  GCCAAGTAGAAGTGATACCTAAACGTTGCTTTCTTTTGCCCCCCAAAACAATTAGATTCC
  CTCCTGAGATCGACAAGCTGGAATTTCCAGGATGACGGGGTGGGGTGGGCAGTAATGTTG
  GAGGGCACTTCACACAAGAGCAAAGCCGTGGGGCCCTGAAGGCCCATCCCCACCGGGGAG
  GTCCACCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015981
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015981.2, NP_057065.2
• RefSeq Size: 4836 bp
• RefSeq ORF: 1470 bp
• Locus ID: 815
• Cytogenetics: 5q32
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1 and 3 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.