Hsp60 (HSPD1) (NM_002156) Human Untagged Clone

CAT#: SC111640

HSPD1 (untagged)-Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_002156" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HSPD1
Synonyms CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111640 sequence for NM_002156 edited (data generated by NextGen Sequencing)
ATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTACTGGCTCCT
CATCTCACTCGGGCTTATGCCAAAGATGTAAAATTTGGTGCAGATGCCCGAGCCTTAATG
CTTCAAGGTGTAGACCTTTTAGCCGATGCTGTGGCCGTTACAATGGGGCCAAAGGGAAGA
ACAGTGATTATTGAGCAGAGTTGGGGAAGTCCCAAAGTAACAAAAGATGGTGTGACTGTT
GCAAAGTCAATTGACTTAAAAGATAAATACAAAAACATTGGAGCTAAACTTGTTCAAGAT
GTTGCCAATAACACAAATGAAGAAGCTGGGGATGGCACTACCACTGCTACTGTACTGGCA
CGCTCTATAGCCAAGGAAGGCTTCGAGAAGATTAGCAAAGGTGCTAATCCAGTGGAAATC
AGGAGAGGTGTGATGTTAGCTGTTGATGCTGTAATTGCTGAACTTAAAAAGCAGTCTAAA
CCTGTGACCACCCCTGAAGAAATTGCACAGGTTGCTACGATTTCTGCAAACGGAGACAAA
GAAATTGGCAATATCATCTCTGATGCAATGAAAAAAGTTGGAAGAAAGGGTGTCATCACA
GTAAAGGATGGAAAAACACTGAATGATGAATTAGAAATTATTGAAGGCATGAAGTTTGAT
CGAGGCTATATTTCTCCATACTTTATTAATACATCAAAAGGTCAGAAATGTGAATTCCAG
GATGCCTATGTTCTGTTGAGTGAAAAGAAAATTTCTAGTATCCAGTCCATTGTACCTGCT
CTTGAAATTGCCAATGCTCACCGTAAGCCTTTGGTCATAATCGCTGAAGATGTTGATGGA
GAAGCTCTAAGTACACTCGTCTTGAATAGGCTAAAGGTTGGTCTTCAGGTTGTGGCAGTC
AAGGCTCCAGGGTTTGGTGACAATAGAAAGAACCAGCTTAAAGATATGGCTATTGCTACT
GGTGGTGCAGTGTTTGGAGAAGAGGGATTGACCCTGAATCTTGAAGACGTTCAGCCTCAT
GACTTAGGAAAAGTTGGAGAGGTCATTGTGACCAAAGACGATGCCATGCTCTTAAAAGGA
AAAGGTGACAAGGCTCAAATTGAAAAACGTATTCAAGAAATCATTGAGCAGTTAGATGTC
ACAACTAGTGAATATGAAAAGGAAAAACTGAATGAACGGCTTGCAAAACTTTCAGATGGA
GTGGCTGTGCTGAAGGTTGGTGGGACAAGTGATGTTGAAGTGAATGAAAAGAAAGACAGA
GTTACAGATGCCCTTAATGCTACAAGAGCTGCTGTTGAAGAAGGCATTGTTTTGGGAGGG
GGTTGTGCCCTCCTTCGATGCATTCCAGCCTTGGACTCATTGACTCCAGCTAATGAAGAT
CAAAAAATTGGTATAGAAATTATTAAAAGAACACTCAAAATTCCAGCAATGACCATTGCT
AAGAATGCAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTATGCAAAGTTCCTCAGAA
GTTGGTTATGATGCTATGGCTGGAGATTTTGTGAATATGGTGGAAAAAGGAATCATTGAC
CCAACAAAGGTTGTGAGAACTGCTTTATTGGATGCTGCTGGTGTGGCCTCTCTGTTAACT
ACAGCAGAAGTTGTAGTCACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA
ATGGGTGGAATGGGAGGTGGTATGGGAGGTGGCATGTTCTAA

Clone variation with respect to NM_002156.4
>OriGene 5' read for NM_002156 unedited
TTTAGGTTTTGTAATACGACTTACTATAGGGCGGCCGACGAATTCGCACGAGGGTCTCGC
CGAGCGCACGCCTTGCCGCCGCCCCGCAGAATGCTTCGGTTACCCACAGTCTTTCGCCAG
ATGAGACCGGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCAAAGATGTA
AAATTTGGTGCAGATGCCCGAGCCTTAATGCTTCAAGGTGTAGACCTTTTAGCCGATGCT
GTGGCCGTTACAATGGGGCCAAAGGGAAGAACAGTGATTATTGAGCAGAGTTGGGGAAGT
CCCAAAGTAACAAAAGATGGTGTGACTGTTGCAAAGTCAATTGACTTAAAAGATAAATAC
AAAAACATTGGAGCTAAACTTGTTCAAGATGTTGCCAATAACACAAATGAAGAAGCTGGG
GATGGCACTACCACTGCTACTGTACTGGCACGCTCTATAGCCAAGGAAGGCTTCGAGAAG
ATTAGCAAAGGTGCTAATCCAGTGGAAATCAGGAGAGGTGTGATGTTAGCTGTTGATGCT
GTAATTGCTGAACTTAAAAAGCAGTCTAAACCTGTGACCACCCCTGAAGAAATTGCACAG
GTTGCTACGATTTCTGCAAACGGAGACCAAGAAATTGGCAATATCATCTCTGATGCAATG
AANAAAGTTGGAAGAAAGGGTGTCATCACAGTAAAGGATGGAAAAACACTGAATGATGAA
TTAGAAATTATTGAAGGCATNGAGTTTGATCGAGGCTATNATTCCCATACTTTATTAATA
CATCANAAGGTCAGAAATGTGAATTNCCAGATGCCTATGTTCTGNTGAGTGAAAAGAAAT
TTCTAGTATCCAGTCCATTGTACCTGCTNCTGAAATTGCAATGCTNACCGNTAGCCTTGG
NTCATNATCGCTGAAGAATGTGATGGAGAAAGCTCTAGTC
>OriGene 3' read for NM_002156 unedited
GCACTTCCAGGGCCGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGT
TCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTA
ACTTTAAACAAATTTTTATTACACAAAGGTTGTCACATAATTGGATACTTCTCTACTTTG
TACACAATTATTCTCACTCTCCACAGAAAGGCTGCTTAACTTCTCATCTGGTGGTGGCAA
GCACTAAAATCCTGATTTTAACAGAATAGTAGTAAAAATGCCTCAGTGATTTAAGTTGAA
AGCAGTACACTGGTACATGGCTCTTGTACCCAGTATCAGGAATGTACAAATGTTTTTTAT
TCAAAAATACAAAATAAATTATCTGTAGGCATGGACAATGACAGCAGTAAACCATTATAT
ATTTTGTCAACTGAAACCAGTAACTGATGGTTATAGTGATTTTCAGCCAGCCTTTTTCTT
CATTTTCTCCAACTGACTTCTCTGAAGTTATTGGTGAGGAACACTGCCTTGGGCTTCCTG
TCACAGTTCATTAATAAAGGTAAAGCACTAGTCTAGGAGTTAGAACATGCCACCTCCCAT
ACCACCTCCCATTCCACCCATTGCACCCATTCCAGGGTCCTTCTCTTCTTTAGGAATTTC
TGTGACTACAACTTCTGCTGTAGTTAACAGAGAGGCCACACCAGCAGCATCCAATAAAGC
AGTTCTCACAACCTTTGNTGGGTCAATGATTCCTTTTTCCACCATATTCACAAAATCTNC
AGCCATAGCATCATAACCCACTTCTGAGGAACTTTGCATAANTTTCTCACTATCAAAGAT
CCTTTCACACCTGCATTCTTAGCATGGNCATTGCTGGAAATTTGGAGTGTCTC
Restriction Sites NotI-NotI     
ACCN NM_002156
Insert Size 2240 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002156.4, NP_002147.2
RefSeq Size 2339 bp
RefSeq ORF 1722 bp
Locus ID 3329
Cytogenetics 2q33.1
Domains cpn60_TCP1
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways RNA degradation, Type I diabetes mellitus
Gene Summary 'This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]'
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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