CD130 (IL6ST) (NM_002184) Human Untagged Clone

CAT#: SC111643

IL6ST (untagged)-Human interleukin 6 signal transducer (gp130, oncostatin M receptor) (IL6ST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL6ST
• Synonyms: CD130; CDW130; GP130; IL-6RB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111643 sequence for NM_002184 edited (data generated by NextGen Sequencing)
  ATGTTGACGTTGCAGACTTGGCTAGTGCAAGCCTTGTTTATTTTCCTCACCACTGAATCT
  ACAGGTGAACTTCTAGATCCATGTGGTTATATCAGTCCTGAATCTCCAGTTGTACAACTT
  CATTCTAATTTCACTGCAGTTTGTGTGCTAAAGGAAAAATGTATGGATTATTTTCATGTA
  AATGCTAATTACATTGTCTGGAAAACAAACCATTTTACTATTCCTAAGGAGCAATATACT
  ATCATAAACAGAACAGCATCCAGTGTCACCTTTACAGATATAGCTTCATTAAATATTCAG
  CTCACTTGCAACATTCTTACATTCGGACAGCTTGAACAGAATGTTTATGGAATCACAATA
  ATTTCAGGCTTGCCTCCAGAAAAACCTAAAAATTTGAGTTGCATTGTGAACGAGGGGAAG
  AAAATGAGGTGTGAGTGGGATGGTGGAAGGGAAACACACTTGGAGACAAACTTCACTTTA
  AAATCTGAATGGGCAACACACAAGTTTGCTGATTGCAAAGCAAAACGTGACACCCCCACC
  TCATGCACTGTTGATTATTCTACTGTGTATTTTGTCAACATTGAAGTCTGGGTAGAAGCA
  GAGAATGCCCTTGGGAAGGTTACATCAGATCATATCAATTTTGATCCTGTATATAAAGTG
  AAGCCCAATCCGCCACATAATTTATCAGTGATCAACTCAGAGGAACTGTCTAGTATCTTA
  AAATTGACATGGACCAACCCAAGTATTAAGAGTGTTATAATACTAAAATATAACATTCAA
  TATAGGACCAAAGATGCCTCAACTTGGAGCCAGATTCCTCCTGAAGACACAGCATCCACC
  CGATCTTCATTCACTGTCCAAGACCTTAAACCTTTTACAGAATATGTGTTTAGGATTCGC
  TGTATGAAGGAAGATGGTAAGGGATACTGGAGTGACTGGAGTGAAGAAGCAAGTGGGATC
  ACCTATGAAGATAGACCATCTAAAGCACCAAGTTTCTGGTATAAAATAGATCCATCCCAT
  ACTCAAGGCTACAGAACTGTACAACTCGTGTGGAAGACATTGCCTCCTTTTGAAGCCAAT
  GGAAAAATCTTGGATTATGAAGTGACTCTCACAAGATGGAAATCACATTTACAAAATTAC
  ACAGTTAATGCCACAAAACTGACAGTAAATCTCACAAATGATCGCTATCTAGCAACCCTA
  ACAGTAAGAAATCTTGTTGGCAAATCAGATGCAGCTGTTTTAACTATCCCTGCCTGTGAC
  TTTCAAGCTACTCACCCTGTAATGGATCTTAAAGCATTCCCCAAAGATAACATGCTTTGG
  GTGGAATGGACTACTCCAAGGGAATCTGTAAAGAAATATATACTTGAGTGGTGTGTGTTA
  TCAGATAAAGCACCCTGTATCACAGACTGGCAACAAGAAGATGGTACCGTGCATCGCACC
  TATTTAAGAGGGAACTTAGCAGAGAGCAAATGCTATTTGATAACAGTTACTCCAGTATAT
  GCTGATGGACCAGGAAGCCCTGAATCCATAAAGGCATACCTTAAACAAGCTCCACCTTCC
  AAAGGACCTACTGTTCGGACAAAAAAAGTAGGGAAAAACGAAGCTGTCTTAGAGTGGGAC
  CAACTTCCTGTTGATGTTCAGAATGGATTTATCAGAAATTATACTATATTTTATAGAACC
  ATCATTGGAAATGAAACTGCTGTGAATGTGGATTCTTCCCACACAGAATATACATTGTCC
  TCTTTGACTAGTGACACATTGTACATGGTACGAATGGCAGCATACACAGATGAAGGTGGG
  AAGGATGGTCCAGAATTCACTTTTACTACCCCAAAGTTTGCTCAAGGAGAAATTGAAGCC
  ATAGTCGTGCCTGTTTGCTTAGCATTCCTATTGACAACTCTTCTGGGAGTGCTGTTCTGC
  TTTAATAAGCGAGACCTAATTAAAAAACACATCTGGCCTAATGTTCCAGATCCTTCAAAG
  AGTCATATTGCCCAGTGGTCACCTCACACTCCTCCAAGGCACAATTTTAATTCAAAAGAT
  CAAATGTATTCAGATGGCAATTTCACTGATGTAAGTGTTGTGGAAATAGAAGCAAATGAC
  AAAAAGCCTTTTCCAGAAGATCTGAAATCATTGGACCTGTTCAAAAAGGAAAAAATTAAT
  ACTGAAGGACACAGCAGTGGTATTGGGGGGTCTTCATGCATGTCATCTTCTAGGCCAAGC
  ATTTCTAGCAGTGATGAAAATGAATCTTCACAAAACACTTCGAGCACTGTCCAGTATTCT
  ACCGTGGTACACAGTGGCTACAGACACCAAGTTCCGTCAGTCCAAGTCTTCTCAAGATCC
  GAGTCTACCCAGCCCTTGTTAGATTCAGAGGAGCGGCCAGAAGATCTACAATTAGTAGAT
  CATGTAGATGGCGGTGATGGTATTTTGCCCAGGCAACAGTACTTCAAACAGAACTGCAGT
  CAGCATGAATCCAGTCCAGATATTTCACATTTTGAAAGGTCAAAGCAAGTTTCATCAGTC
  AATGAGGAAGATTTTGTTAGACTTAAACAGCAGATTTCAGATCATATTTCACAATCCTGT
  GGATCTGGGCAAATGAAAATGTTTCAGGAAGTTTCTGCAGCAGATGCTTTTGGTCCAGGT
  ACTGAGGGACAAGTAGAAAGATTTGAAACAGTTGGCATGGAGGCTGCGACTGATGAAGGC
  ATGCCTAAAAGTTACTTACCACAGACTGTACGGCAAGGCGGCTACATGCCTCAGTGA
  
  Clone variation with respect to NM_002184.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002184 unedited
  TAATTACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCTGAACCGGGGGCCGCGCCTGCCAGGCCGACGGGTCTGGCCCA
  GCCTGGCGCCAAGGGGTTCGTGCGCTGTGGAGACGCGGAGGGTCGAGGCGGCGCGGCCTG
  AGTGAAACCCAATGGAAAAAGCATGACATTTAGAAGTAGAAGACTTAGCTTCAAATCCCT
  ACTCCTTCACTTACTAATTTTGTGATTTGGAAATATCCGCGCAAGATGTTGACGTTGCAG
  ACTTGGCTAGTGCAAGCCTTGTTTATTTTCCTCACCACTGAATCTACAGGTGAACTTCTA
  GATCCATGTGGTTATATCAGTCCTGAATCTCCAGTTGTACAACTTCATTCTAATTTCACT
  GCAGTTTGTGTGCTAAAGGAAAAATGTATGGATTATTTTCATGTAAATGCTAATTACATT
  GTCTGGAAAACAAACCATTTTACTATTCCTAAGGAGCAATATACTATCATAAACAGAACA
  GCATCCAGTGTCACCTTTACAGATATAGCTTCATTAAATATTCAGCTCACTTGCAACATT
  CTTACATTCGGACAGCTTGAACAGAATGTTTATGGAATCACAATAATTTCAGGCTTGCCT
  CCAGAANAACCTAAAAATTTGAGTTGCATTGTGAACGAGGGGAAGAACACTGAGTGTGAG
  TGGGAGGGTNGNAANGGGAAACACACTTGGNAGACAACTTCACTTTAAATCTGAATGGGC
  ACACACCAGTTTGCTGATTGCAAGCAAACGTGACACCCCCCTCTGCACTGTTGATATTCT
  ACGGGGTATTTGCACCAATGAATCTGGTAAACCAAGATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002184
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002184.2, NP_002175.2
• RefSeq Size: 3242 bp
• RefSeq ORF: 2757 bp
• Locus ID: 3572
• Cytogenetics: 5q11.2
• Domains: FN3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: 'The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.