OTX2 (NM_021728) Human Untagged Clone

CAT#: SC111661

OTX2 (untagged)-Human orthodenticle homeobox 2 (OTX2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OTX2
• Synonyms: CPHD6; MCOPS5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  GCCCTCACTCGCCACATCTACTTTGATAGCTGGCTATTTGGAATTTAAAGGATATTTGACTTTTTCTAAC
  CTCCCATGAGGCTGTAAGTTCCACTGCTCCAAACCCACCCACCAAGGACTCTGAACCTGTCCACCCCGGG
  CGCATCAAGATCTTCCAGCTGGGTACCCCCGATTTGGGCCGACTTTGCACCTCCAAACAACCTTAGCATG
  ATGTCTTATCTTAAGCAACCGCCTTACGCAGTCAATGGGCTGAGTCTGACCACTTCGGGTATGGACTTGC
  TGCACCCCTCCGTGGGCTACCCGGGGCCCTGGGCTTCTTGTCCCGCAGCCACCCCCCGGAAACAGCGCCG
  GGAGAGGACGACGTTCACTCGGGCGCAGCTAGATGTGCTGGAAGCACTGTTTGCCAAGACCCGGTACCCA
  GACATCTTCATGCGAGAGGAGGTGGCACTGAAAATCAACTTGCCCGAGTCGAGGGTGCAGGTATGGTTTA
  AGAATCGAAGAGCTAAGTGCCGCCAACAACAGCAACAACAGCAGAATGGAGGTCAAAACAAAGTGAGACC
  TGCCAAAAAGAAGACATCTCCAGCTCGGGAAGTGAGTTCAGAGAGTGGAACAAGTGGCCAATTCACTCCC
  CCCTCTAGCACCTCAGTCCCGACCATTGCCAGCAGCAGTGCTCCTGTGTCTATCTGGAGCCCAGCTTCCA
  TCTCCCCACTGTCAGATCCCTTGTCCACCTCCTCTTCCTGCATGCAGAGGTCCTATCCCATGACCTATAC
  TCAGGCTTCAGGTTATAGTCAAGGATATGCTGGCTCAACTTCCTACTTTGGGGGCATGGACTGTGGATCA
  TATTTGACCCCTATGCATCACCAGCTTCCCGGACCAGGGGCCACACTCAGTCCCATGGGTACCAATGCAG
  TCACCAGCCATCTCAATCAGTCCCCAGCTTCTCTTTCCACCCAGGGATATGGAGCTTCAAGCTTGGGTTT
  TAACTCAACCACTGATTGCTTGGATTATAAGGACCAAACTGCCTCCTGGAAGCTTAACTTCAATGCTGAC
  TGCTTGGATTATAAAGATCAGACATCCTCGTGGAAATTCCAGGTTTTGTGAAGACCTGTAGAACCTCTTT
  TTGTGGGTGATTTTTAAATATACTGGGCTGGACATTCCAGTTTTAGCCAGGCATTGGTTAAAAGAGTTAG
  ATGGGATGATGCTCAGACTCATCTGATCAAAGTTCCGAGAGGCATAGAAGGAAAAACGAAGGGCCTTAGA
  GGGGCCTACAAACCAGCAACATGAAATGGACAAACCAATCTGCTTAAGATCCTGTCATAGTTTTAGATCA
  TTGGTTATCCTGATTTGCAAAGTGATCAAAAGCATTCTAGCCATGTGCAACCAAACACCACCAAAAATAA
  AATCAAACAAAACTAAGTTGTGAAGGAAGGGAGGGAAGGTCATAGCCTTCTTAAGCAGAGGTGTTCCATT
  GTTTTAGCCAATCCTTGGTTGAATCTTAGGAATGAACAGTGTCTCAAGCTCATTCACGTTTCATGACCAA
  CTGGTAGTTGGCACTGAAAAAACTTTTCAGGGCTGTGTGAATTGTGTGACTGATTGTCCTAGATGCACTA
  CTTTATTTAAAAAATAATGTTCATAAGGAGTCAATATGTAGTTTAAGAGACAATCAGTGTGTGTCTTATA
  AATGGTACATCTGTGGTTTTTAATCTGTGCTAGACTTCAAAACTGTGATCTCCTGTTATTGTATGCAACC
  TTGAACTCCACCTCTGCAGGGGTTCTTCTGTGATTAAATAGGTTATAATTATAAGCAAAATTCAGAGCAA
  CTGAGTACTGATCTAAAAAGATTACCTTTGGCTGGAGGTGAGCTGCACTGAAACTTTACGACAAAATGTC
  TCTGGMCAAAGAGAGTCAGAGAAGAGAAGCAAAAGGACACTAATTCATCTGTAATTTACTGTTGGTAAGC
  CTAGCAGTAAAGAGACATTGGTCAATTGCTCTGACCCTGATGAATTATTAAACTGAGATCATTGTCGTTT
  ATGCTTGCAGATGTTAAATGGAAAAGTTATATATGCATAAACCTTTTCTTCCTGGATTTGGCAGATATGT
  ATAATTATATTAAAATGGTTCTAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021728 unedited
  CCTCGNGATTTTGTAAACCGNACTTACTGATAGGGCGGACCGCCCAATTCGCACGAGGGC
  CCTCACTTTCCACATCTACTTTGATAGCTGGCTATTTGGAATTTAAAGGATATTTGACTT
  TTTCTAACCTCCCATGAGGCTGTAAGTTCCACTGCTCCAAACCCACCCACCAAGGACTCT
  GAACCTGTCCACCCCGGGCGCATCAAGATCTTCCAGCTTTTTTCCCCCGATTTGGGCCGA
  CTTTGCACCTCCAAACAACCTTAGCATGATGTCTTATCTTAAGCAACCGCCTTACGCAGT
  CAATGGGCTGAGTCTGACCACTTCGGGTATGGACTTGCTGCACCCCTCCGTGGGCTACCC
  GGGGCCCTGGGCTTCTTGTCCCGCAGCCACCCCCCGGAAACAGCGCCGGGAGAGGACGAC
  GTTCACTCGGGCGCAGCTAGATGTGCTGGAAGCACTGTTTGCCAAGACCCGGTACCCAGA
  CATCTTCATGCGAGAGGAGGTGGCACTGAAAATCAACTTGCCCGAGTCGAGGGTGCAGGT
  ATGGTTTAAGAATCGAAGAGCTAAGTGCCGCCAACAACAGCAACAACAGCAGAATGGAGG
  TCAAAACAAAGTGAGACCTGCCAAAAAGAAGACATCTCCAGCTCGGGAAGTGAGTTCAGA
  GAGTGGAACAAGTGGCCAATTCACTCCCCCCTCTAGCACCTCAGTCCCGACCATTGCCAG
  CAGCAGTGCTCCTGTGTCTATCTGGAGCCCAGCTTCCATCTCCCCACTGTCAGATCCCTT
  GTCCACCTCCTCTTCCTGCATGCAGAGGTCCTATCCCATGACCTATACTCANGCTTCANG
  TTATAGTCAAGGATATGCTGGCTCAACTTTCTACTTTGNGGCATGGACTGTGNATCATAT
  TTGACCCCTATGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021728 unedited
  NGGTACAGATATGNNACCGCGGCCGCATNCNAGNGATCGGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTGGGCTAGAACCATTTTAATATAATTATACATATCTGCCAAATCCAGGAAAAAA
  AGTTTATGCATATATAACTTTTCCATTTAACATCTGCAAGCATAAACGACAATGATCTCA
  GTTTAATAATTCATCAGGGTCAGAGCAATTGACCAATGTCTCTTTACTGCTAGGCTTACC
  AACAGTAAATTACAGATGAATTAGTGTCCTTTTGCTTCTCTTCTCTGACTCTCTTTGTCC
  AGAGACATTTTGTCGTAAAGTTTCAGTGCAGCTCACCTCCAGCCAAAGGTAATCTTTTTA
  GATCAGTACTCAGTTGCTCTGAATTTTGCTTATAATTATAACCTATTTAATCACAGAAGA
  ACCCCTGCAGAGGTGGAGTTCAAGGTTGCATACAATAACAGGAGATCACAGTTTTGAAGT
  CTAGCACAGATTAAAAACCACAGATGTACCATTTATAAGACACACACTGATTGTCTCTTA
  AACTACATATTGACTCCTTATGAACATTATTTTTTAAATAAAGTAGTGCATCTAGGACAA
  TCAGTCACACAATTCACACAGCCCTGAAAAGTTTTTTCAGTGCCAACTACCAGTTGGTCA
  TGAAACGTGAATGAGCTTGAGACACTGTTCATTCCTAAGATTCAACCAAGGATTGGCTAA
  AACAATGGAACACCTCTGCTTAAGAAGGCTATGACCTTCCCTCCCTTCCTTCACAACTTA
  GTTTTGTTTGATTTTATTTTTGGGTGGTGTTTTGGTTGCACATGGCTAGAATGCTTTTTG
  ATCACTTTGCNAATCAGGATAACCAATGATCTAAAACTATGACAGGATCTTAAGCAGATG
  GNTTTGGCCATTTCATGTGCTGGTTTGTGAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021728
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021728.2, NP_068374.1
• RefSeq Size: 2209 bp
• RefSeq ORF: 894 bp
• Locus ID: 5015
• Cytogenetics: 14q22.3
• Domains: homeobox, TF_Otx
• Protein Families: Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: 'This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 5 encode the same protein (isoform a).