PKIB (NM_032471) Human Untagged Clone

CAT#: SC111674

PKIB (untagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKIB
• Synonyms: PRKACN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111674 sequence for NM_032471 edited (data generated by NextGen Sequencing)
  ATGAGGACAGATTCATCAAAAATGACTGACGTGGAGTCTGGGGTCGCCAATTTTGCATCT
  TCAGCAAGGGCAGGCCGCCGGAATGCCTTACCAGACATCCAGAGTTCAGCTGCCACAGAC
  GGAACCTCAGATTTGCCCCTCAAACTGGAGGCTCTCTCCGTGAAGGAAGATGCAAAAGAG
  AAAGATGAAAAAACAACACAAGACCAATTGGAAAAGCCTCAAAATGAAGAAAAATGA
  
  Clone variation with respect to NM_032471.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032471 unedited
  ACTCGGATTTGTAAACGACTTATATAGGCGGCACCGCAATTCGCACGAGGAGCGAGCTAG
  AGCCCGAGTCGCAGCTCCGGGCCGCAGAGCGCTGGGCGAGCGCGAGCGCCAGGGCACCGG
  CAGGGCAGGCAGCTGCGCGCGGCTGGAGTCATGCTATACTGAAAAGACACTTCATCAAGA
  TAACTCTGGGAGAAGCAGAAAACCCTGTGCCATGGACAGGAAAGATAGGAGAAAGAAAGT
  TTATCAGAATTTTTTAAACCTGTCTCAGAAATAACAACATATTTTAATCAGAGATTTATG
  TTGCTATGAGGACAGATTCATCAAAAATGACTGACGTGGAGTCTGGGGTCGCCAATTTTG
  CATCTTCAGCAAGGGCAGGCCGCCGGAATGCCTTACCAGACATCCAGAGTTCAGCTGCCA
  CAGACGGAACCTCAGATTTGCCCCTCAAACTGGAGGCTCTCTCCGTGAAGGAAGATGCAA
  AAGAGAAAGATGAAAAAACAACACAAGACCAATTGGAAAAGCCTCAAAATGAAGAAAAAT
  GAAGGCTCATAATCTATCAAGAGTGCTGAATTTCTGCATGTTGAAAGACTTAGTGGTTCT
  GTTTTCTTGAGACATTTAATCTGGTGGTAACTGTGGTAACATTGCAGCCCTAAGCAGCAT
  GTGTATATTAGATAATTGTGTTGTGATGCTACTCACTTTGATTGGCATGATGATGTCCAA
  GGTAAGCTATTNAAAGGCANGNTACTTCNCAATCGCACTGNAGGGAAAGGGTNAGAAATA
  TACATGATCACAGNAATGCATACCACTGNTCTGTAACCCAACAAATTCACTGGTCTCTTT
  TGGATTTATTTAGCCTGATGTATTTTTAATTCAATTTTTATGGTGATGGGGCAATCATNC
  TTGGTAAATGTAAATCAAACATGATTGATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032471 unedited
  GTACGCGGCCGCCTTTAGAGTCGAGNNCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAGGGAAAATAGTAAAATTCGTTTAATAGGCATGTTCCATATAACC
  ATTTTGGTACAAATTTTGCATTTTCCACATGGAAAAAAATCACAGGGGGCCCATACTAAA
  AGCAAAATACGGCAGACAAAAATATCCTAAAGATGGTTCTGGTATCAAACTTTTACAATT
  TTTCCAAAAAGTTTTTGAGGGTTGGGAAAAAGTCTGGGGCATTTTTGGCAAAAAACAAAC
  ACACTCTATCCATGGGAGTTTTGACTATTGGTCTTTCTCACCAAAAATGGCCATAATTTT
  CTACAAATTCCATGAAGGTTTAAATCAATCATGTTTGATTTACATTTACCAAGAATGATT
  TGCCCCTCACCCTAAAAATTGAATTAAAAATACCTCAGGCTAAATAAATCCAAAAGAGAA
  CAGGGAATTTTGTTGGGTTTACAGACAGGGGTATGCATTTCTGGGATCATGTATTATTCT
  TAACCTTTTCCTTCAGCGCGATTTGGAAGGAACCCGCCTTTTATTAGCTTACCTTGGACA
  TTATCATTGGAATCAAAGTGAGTAGCTTCACAACACAATTATTCTATATACACATGCTGC
  TTAGGGCTGCATGTTACCACAGTTACCACCAAATAAAATGTCTCAAGAAACAGGACCCCT
  AAGTCTTTTACATGCAGAAATTCAGACTCTGGATAGATATGAGCCCTTATTTTTTTAATT
  TGGAGGCTTTCCAATTGGCCTGGGGGGGTTTTTATCTTTCTTTTTGAATTTCCTTCCCGA
  GAGAGCCCCGTTTGAGGGCAAATTGAAGTTCCGCCGGGGAACTAACTCTGGTGGTTGGTA
  GGCTTTCGCGGCCCGCCCTTCGTAAAGCAATTGGGACCCAACTCCGGAGGCCTTTGGAGA
  ATTGCCCCAAAACAAACCTCGTAAAAATGGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032471
• Insert Size: 1220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032471.4, NP_115860.1
• RefSeq Size: 1842 bp
• RefSeq ORF: 237 bp
• Locus ID: 5570
• Cytogenetics: 6q22.31
• Domains: PKI
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the cAMP-dependent protein kinase inhibitor family. The encoded protein may play a role in the protein kinase A (PKA) pathway by interacting with the catalytic subunit of PKA, and overexpression of this gene may play a role in prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.