Staufen (STAU1) (NM_004602) Human Untagged Clone

CAT#: SC111694

STAU1 (untagged)-Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAU1
• Synonyms: PPP1R150; STAU
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111694 sequence for NM_004602 edited (data generated by NextGen Sequencing)
  ATGAAACTTGGAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTCGGATGCAGTCC
  ACCTATAACTACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTTACCCATTTCCA
  GTTCCACCTTTACTTTATCAAGTGGAACTTTCTGTGGGAGGACAGCAATTTAATGGCAAA
  GGAAAGACAAGACAGGCTGCGAAACACGATGCTGCTGCCAAAGCGTTGAGGATCCTGCAG
  AATGAGCCCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAGAATCCGAAGAAGAAAATCTC
  AATAAATCTGAAATAAGTCAAGTGTTTGAGATTGCACTTAAACGGAACTTGCCTGTGAAT
  TTCGAGGTGGCCCGGGAGAGTGGCCCACCCCACATGAAGAACTTTGTGACCAAGGTTTCG
  GTTGGGGAGTTTGTGGGGGAAGGTGAAGGGAAAAGCAAGAAGATTTCAAAGAAAAATGCC
  GCCATAGCTGTTCTTGAGGAGCTGAAGAAGTTACCGCCCCTGCCTGCAGTTGAACGAGTA
  AAGCCTAGAATCAAAAAGAAAACAAAACCCATAGTCAAGCCACAGACAAGCCCAGAATAT
  GGCCAGGGGATCAATCCGATTAGCCGACTGGCCCAGATCCAGCAGGCAAAAAAGGAGAAG
  GAGCCAGAGTACACGCTCCTCACAGAGCGAGGCCTCCCGCGCCGCAGGGAGTTTGTGATG
  CAGGTGAAGGTTGGAAACCACACTGCAGAAGGAACGGGCACCAACAAGAAGGTGGCCAAG
  CGCAATGCAGCCGAGAACATGCTGGAGATCCTTGGTTTCAAAGTCCCGCAGGCGCAGCCC
  ACCAAACCCGCACTCAAGTCAGAGGAGAAGACACCCATAAAGAAACCAGGGGATGGAAGA
  AAAGTAACCTTTTTTGAACCTGGCTCTGGGGATGAAAATGGGACTAGTAATAAAGAGGAT
  GAGTTCAGGATGCCTTATCTAAGTCATCAGCAGCTGCCTGCTGGAATTCTTCCCATGGTG
  CCCGAGGTCGCCCAGGCTGTAGGAGTTAGTCAAGGACATCACACCAAAGATTTTACCAGG
  GCAGCTCCGAATCCTGCCAAGGCCACGGTAACTGCCATGATAGCCCGAGAGTTGTTGTAT
  GGGGGCACCTCGCCCACAGCCGAGACCATTTTAAAGAATAACATCTCTTCAGGCCACGTA
  CCCCATGGACCTCTCACGAGACCCTCTGAGCAACTGGACTATCTTTCCAGAGTCCAGGGA
  TTCCAGGTTGAATACAAAGACTTCCCCAAAAACAACAAGAACGAATTTGTATCTCTTATC
  AATTGCTCCTCTCAGCCACCTCTGATCAGCCATGGTATCGGCAAGGATGTGGAGTCCTGC
  CATGATATGGCTGCGCTGAACATCTTAAAGTTGCTGTCTGAGTTGGACCAACAAAGTACA
  GAGATGCCAAGAACAGGAAACGGACCAATGTCTGTGTGTGGGAGGTGCTGA
  
  Clone variation with respect to NM_004602.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004602 unedited
  NGTTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGCCGGCGGCT
  GCCGCGTCTCTCTCGGCTCCCGCTTCCTTTGACCGCCTCCCCCCCCCGGCCCGGCGGCGC
  CCGCCTCCTCCACGGCCACTCCGCCTCTTCCCTCCCTTCGTCCCTTCTTCCTCTCCCTTT
  TTTCCTTCTTCCTTCCCCTCCTCGCCGCCACCGCCCAGGACCGCCGGCCGGGGGACGAGC
  TCGGAGCAGCAGCCAGAGTTTATTAACCACTTAACCTCTCAGAACTGAACAAAGACAACA
  TTGTTCCTGGAACGCCCTCTTTTTAAAAAAGAAAGCATAACCCCTACTGTAGAACTAAAT
  GCACTGTGCATGAAACTTGGAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTCGG
  ATGCAGTCCACCTATAACTACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTTAC
  CCATTTCCAGTTCCACCTTTACTTTATCAAGTGGAACTTTCTGTGGGAGGACAGCAATTT
  AATGGCAAAGGAAAGACAAGACAGGCTGCGAAACACGATGCTGCTGCCAAAGCGTTGAGG
  ATCCTGCAGAATGAGCCCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAAGATCCGAAGAA
  GAAATCTCAATAAATCTGAAATAAGTCAAGTGTTTGAGAATGCACTTAAACGGAAACTGC
  CTGTGAATTTCGAGGTGGCCCGGGAGAGTGGNCCACCCCACATGAAAGAACTTGTGACCA
  AGNTTTCGGTTGGNNGAAGTTTGTGGGGGAAGGTGAAAGGGAAAGCNAGAAGATTNCAAG
  AANAAAGCCGCCATAGCTGNTCTTGAGGAGCTGAAGAAATTACCGCCCCTGCCTGCAGNT
  GAACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004602 unedited
  GCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAATTAGTAGCTGTTTATTGATCA
  ATGGTTTGATATAAAGTTATTTCAGATCTTCAGACTTTTGCCCAGATGGAATCACAAGCA
  TTACAAAGTTTTTTCTTAAAAATAAAAAAAGGATAGGGGCAAGTTGGGAGGGGACCAACC
  TAGCAGTAGTGGCATTTGAGAATAAATTAACAAAAAAATTTAGTATTACCATTTATTGAT
  GACAAACACTTAAGTTTTACTTACATTCCATGGGGAGAAAAATTCCAGCGTAAACAATGA
  ATGGAAGCAGTACTTAACTCGCAGGGCTACCAGGCTTTCCATACGGACCACACGCAGAGC
  CTCAGTGCACACACTTCTGTGTACAGTAACACAACATCAAAAGCAACACAGCTGTATACA
  GAAACGTAGGTCATTCTTTTCAGCCCTAATGGAGATGTAATTAACAGTATCGAGCACTCT
  GGAAAATCACTCTGCAGGTTTATATGGACTACATGGAGATCATATCCTGTAGTGTAGTGA
  AAGCTAAGTCCTCAAGAGCCATATGTATAGATACACAATGCTTTTTAATAATCTTTAAAA
  CAGAGATCAAAGTTCATTTAAGTCCTGTTTTGCTTAACAAAAATAAAAATGAAATAAAAA
  TGGACCAAATGATCTTCCAAGTTTAAAATTCCTAATTGCCCCATTTATACAACTGTGGGA
  AGACTAATTAAAGGTTTTTTGAAAGCCAGGACTGGTTTACCTTGACCAAAGGGCCCACAT
  TTGCATTACTGAAACCGTCCTGGGTAACCCAATGAAATAAAATGGTTCACCCAATACACC
  GGATTTGAACTTTGCCGGTAAAATAATTGAAAAGTCAGTTTAAGAAAGTTCCAAACGGGG
  CTTTTAACTAAATCAATTGGAAAATTTCAATGGAACATTTTTTCGGGCCTATCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004602
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004602.1, NP_004593.1
• RefSeq Size: 3349 bp
• RefSeq ORF: 1491 bp
• Locus ID: 6780
• Cytogenetics: 20q13.13
• Domains: DSRM
• Gene Summary: 'Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]'
  Transcript Variant: This variant (T4) has an additional exon in the 5' UTR compared to T2, the most abundantly expressed transcript variant. T4 encodes the same isoform (a) of 496 amino acids as T2.