UBE2D2 (NM_003339) Human Untagged Clone

CAT#: SC111700

UBE2D2 (untagged)-Human ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 1


  "NM_003339" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol UBE2D2
Synonyms E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4; UBCH5B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111700 sequence for NM_003339 edited (data generated by NextGen Sequencing)
ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAG
TGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCA
AATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTAC
CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT
AATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCA
AAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTG
CCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAA
TGGACTCAGAAGTATGCGATGTAA

Clone variation with respect to NM_003339.2
>OriGene 5' read for NM_003339 unedited
TGTATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCTGGGAGGAAGAGGCAG
CTACGGCGGCGGCGGCGGTGGCGGCTAGGGCGGCGGCGAATAAAGGGGCCGCCGCCGGGT
GATGCGGTGACCGCTGCGGCAGGCCCAGGAGCTGAGTGGGCCCCGGCCCTCAGCCCGTCC
CGCCGGACCCGCTTTCCTCAACTCTCCATCTTCTCCTGCCGACCGAGATCGCCGAGGCGG
CCTCAGGCTCCCTAGCCCCTTCCCCGTCCCTTCCCCGCCCCCGTCCCCGCCCCGGGGGCC
GCCGCCACCCGCCTCCCACCATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGG
CACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGC
AAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAA
TTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTT
ATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGT
CTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGGTGTGATCCC
AATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAG
TACAACAGAATAGCTCGGGGATGGACTCANAAGTATGCGATGTAATTAAAGAAATTATTG
GATAACCTCTACAATAAAGATAGGGGGACTCTGANAGAGAAAGTCCTTTTGATTTCATTT
GACTGCTTTCTATGAGCCACGCCTCATCTTNCCCTGTGCACATGTTACTGATACAGAGGG
GNTGGCGTGGTGGACTA
>OriGene 3' read for NM_003339 unedited
ACTTCCAGGGCCGGAAAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC
AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTATAT
GTAATGACTGTAGTAACCAGTTTATTACACAGATTAATCATTCTTGAAAGTACAAGCTCC
AGAGGAGCAATTGGGTCTTTAAATATACACAAGTATTTCCATCAAATGAATTTTCACCCT
TACATCCAAATAGACCTAAGCGGTTAAAAACATAAGAAAAATAAGAGCTATTAGCTATGT
ATTAACTGAGAAACCACATACAAACCAAAGAATATGGAAGAGAGAAAGAAGGGCTGAAAG
GCCAAAGGTTGAAGGGGTAGGGAGATGTAAAAGAAGTTGGGAACAAAGCCCATCACACTT
GATGTACTAATAGCTCCAATCCATTTAAATGTTGACCAGTTAAACTTAGACCTTAAAATG
CAGGATGTGGGTAGAGTTTAATCTGGTTGGTCATAACTTTCACCTAAGACGTAGCTCCTT
CGGAATAAAATGAATACAGACACAGCTTCATGTTCTTCACCTTGCTTTTCATAACTGTTT
TGAGTTTAAATGGATTCCACTTAAAAATAATCTCCTATAGGTGAGTGGAGCCTTTTCTTT
TCCATCCAAGCACACACCATACCCACCACCCCCCTTACACCTTGCTGNTATCCAAATTTA
AGTCAATAAGAAAAGCTTTTAGTCTCACACCTAGGTAATGCCTGTAGAACTGGCTTGTCA
CACACACACTTCTCTGCTNAGAGGCNAATGTTGTACAAAAGTACAGTATTTCTAGTTTTG
TGTTCAAGTATGTACACACGCAGCACTGCTGTATCAGGTAACATGTGCCAGGGGAAGATG
ANGCGTGGCTCATAAAAGCAGTCAATGAAATCAAAGGACTTNCTTTTCAAAGTCCCTTNC
TTATTTGGAAAAGGTTNCAATAATNTCTTATAACTCGCATCTTCTGAGACC
Restriction Sites NotI-NotI     
ACCN NM_003339
Insert Size 1700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003339.2, NP_003330.1
RefSeq Size 2715 bp
RefSeq ORF 444 bp
Locus ID 7322
Cytogenetics 5q31.2
Domains UBCc
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary 'Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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