UCP3 (NM_003356) Human Untagged Clone

CAT#: SC111702

UCP3 (untagged)-Human uncoupling protein 3 (mitochondrial, proton carrier) (UCP3), nuclear gene encoding mitochondrial protein, transcript variant long

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCP3
• Synonyms: SLC25A9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111702 sequence for NM_003356 edited (data generated by NextGen Sequencing)
  ATGGTTGGACTGAAGCCTTCAGACGTGCCTCCCACCATGGCTGTGAAGTTCCTGGGGGCA
  GGCACAGCAGCCTGTTTTGCTGACCTCGTTACCTTTCCACTGGACACAGCCAAGGTCCGC
  CTGCAGATCCAGGGGGAGAACCAGGCGGTCCAGACGGCCCGGCTCGTGCAGTACCGTGGC
  GTGCTGGGCACCATCCTGACCATGGTGCGGACTGAGGGTCCCTGCAGCCCCTACAATGGG
  CTGGTGGCCGGCCTGCAGCGCCAGATGAGCTTCGCCTCCATCCGCATCGGCCTCTATGAC
  TCCGTCAAGCAGGTGTACACCCCCAAAGGCGCGGACAACTCCAGCCTCACTACCCGGATT
  TTGGCCGGCTGCACCACAGGAGCCATGGCGGTGACCTGTGCCCAGCCCACAGATGTGGTG
  AAGGTCCGATTTCAGGCCAGCATACACCTCGGGCCATCCAGGAGCGACAGAAAATACAGC
  GGGACTATGGACGCCTACAGAACCATCGCCAGGGAGGAAGGAGTCAGGGGCCTGTGGAAA
  GGAACTTTGCCCAACATCATGAGGAATGCTATCGTCAACTGTGCTGAGGTGGTGACCTAC
  GACATCCTCAAGGAGAAGCTGCTGGACTACCACCTGCTCACTGACAACTTCCCCTGCCAC
  TTTGTCTCTGCCTTTGGAGCCGGCTTCTGTGCCACAGTGGTGGCCTCCCCGGTGGACGTG
  GTGAAGACCCGGTATATGAACTCACCTCCAGGCCAGTACTTCAGCCCCCTCGACTGTATG
  ATAAAGATGGTGGCCCAGGAGGGCCCCACAGCCTTCTACAAGGGATTTACACCCTCCTTT
  TTGCGTTTGGGATCCTGGAACGTGGTGATGTTCGTAACCTATGAGCAGCTGAAACGGGCC
  CTGATGAAAGTCCAGATGTTACGGGAATCACCGTTTTGA
  
  Clone variation with respect to NM_003356.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003356 unedited
  ACAGTAGTTTTGTAAAACCCACGCTCTGGGGTGGCGGCAAAAAACTTCGGCACGAGGATA
  TTTAGCACATAGTAGGCACAAAGAAATGACATTATTATTAAGAGACCTGGGAGAGCTGTG
  CCCAGCCTATCGTGGGAGGCCTTGACCTTTGGACTCAAAAGTGGCAGCAGGTCCACCCCC
  CCATACACCCTTGTCACCAAGGAAGCGTCCACAGCTTATAGTCGTTATATTAAAGCACCC
  CAAGTCAAGAGGACTGAACCAGATCTGGAACTCACTCACCTCCCCTCTCACCTCACTGCC
  CTCACCAGCCAGCCTCTTGTCAAGTGATCAGGCTGTCAACCAACTTCTCTAGGATAAGGT
  TTCAGGTCAGCCCGTGTGTATAAGACCAGTGCCAAGCCAGAAGCAGCAGAGACAACAGTG
  AATGACAAGGAGGGGCCATCCAATCCCTGCTGCCACCTCCTGGGATGGAGCCCTAGGGAG
  CCCCTGTGCTGCCCCTGCCGTGGCAGGACTCACAGCCCCACCGCTGCACTGAAGCCCATG
  GCTGTGGAGCAGCCTCTCTCCTTGGACCTCCTCTCGGCCCTAAAGGGACTGGGCAGAGCC
  TTCCAGGACTATGGTTGGACTGAAGCCTTCAGACGTGCCTCCCACCATGGCTGTGAAGTT
  CCTGGGGGCAAGCACAGCAGCCTGTTTTGCTGACCTCGTTACCTTTCCACTGGACACAGC
  CAATGTCCGCCTGCAGATCCAGGGGGGAGAACCATGCGGTCCAGACAGCCCGGCTCGTGC
  AGTACCGTGGCGTGCTGGGCACCATCCCTGACATGGTGCGGACTGAGGGTCCCTGCAGCC
  CCTACAATGGGCTGGTGGCCCGGGCTGCAACGCCCAGATGAGCCTCGCCTNCATCCCGAT
  AGGCCTCTATGACTTCGTCAAGCAGTGTACACCCCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003356 unedited
  GGCACGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTACTCCCACACCTAAGGTG
  TGAATTCTTTTATTGAGTCATAATAATTTCCCGAGAATTCCGAGTCCTGCTACTTTAGGT
  TCTTGCCCAGGAATCCACCTCTTTTCCCCCAAGCCCAACAATCCTTTGAGGTACTCATGA
  TTGAGCGCGTGGTGGGGGGGGGTGGGGAAGAGGCTGCATGGGGGTGGGGCTCCTGTGGTT
  TCACGTTATTCACTGTAACCTCTGGTCCCCAAGAATATGGATCCTTTGGTATCACCTATA
  CACAACCGAATATTCCAAACCTTCTTCCCTGGCAACTACTCTCTGCCCCGACTCAATCTC
  TTCCAAGGCCCTTGCTCTTGCTCTTACATTCACACACAGCTTGTGCTTTTATAAAGCGCG
  TCTATGCCTACCTTACCACTTGCCTGCCTTACTCCTTCTGGCGCCGCGGACAGGCAAGGG
  ACGGCCCCCTCCCTCCCGCACCAAAGTGGCGACTGCCCTTTCGCTTCTCCCTCGCCTTCT
  CGTCTACTCCTCTATCTCTTCCCTAATTGGCGCCCGTCCGCACCTCCTCACATAGCTACA
  CCCTCCTCCCTCGACCTCGCTCTCCCCACACCCCTCTTGTGTCATCGTCGCCTCCTTCCA
  TCCTTTCTCGTCTCCGACTCTTTTACGTTCACACTCCCCCCTCCAACTTCCGCTCCTTCT
  ATCCCCCCTCCTTCCCCCCCCCTCCCCTTCTCTTTATCTCATTTTCTCTTTTATTTCTCT
  TCTCTCCTCTCTTTTTCCCCCCTCTCTCTCTTCTTCTCCTACTCCATATATTTCCTCTTT
  CTTTCCCACCCCTCAACCATTCCTTCTCTCTCTCCCCTCCACTTCCACTTCCACTTTCCC
  GTTCACCTCCTCATCTACTTTTTTCTACTATTGTCCACCCCTCACTCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003356
• Insert Size: 2660 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003356.2, NP_003347.1
• RefSeq Size: 2263 bp
• RefSeq ORF: 939 bp
• Locus ID: 7352
• Cytogenetics: 11q13.4
• Domains: mito_carr
• Protein Families: Druggable Genome
• Gene Summary: 'Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (long) encodes the full length isoform.