B3GALNT1 (NM_003781) Human Untagged Clone

CAT#: SC111717

B3GALNT1 (untagged)-Human beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group) (B3GALNT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B3GALNT1
• Synonyms: B3GALT3; beta3Gal-T3; galT3; Gb4Cer; GLCT3; GLOB; P; P1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111717 sequence for NM_003781 edited (data generated by NextGen Sequencing)
  ATGGCCTCGGCTCTCTGGACTGTCCTTCCGAGTAGGATGTCACTGAGATCCCTCAAATGG
  AGCCTCCTGCTGCTGTCACTCCTGAGTTTCTTTGTGATGTGGTACCTCAGCCTTCCCCAC
  TACAATGTGATAGAACGCGTGAACTGGATGTACTTCTATGAGTATGAGCCGATTTACAGA
  CAAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTCTCATCAAAATCCATTTCTG
  GTCATTCTGGTGACCTCCCACCCTTCAGATGTGAAAGCCAGGCAGGCCATTAGAGTTACT
  TGGGGTGAAAAAAAGTCTTGGTGGGGATATGAGGTTCTTACATTTTTCTTATTAGGCCAA
  GAGGCTGAAAAGGAAGACAAAATGTTGGCATTGTCCTTAGAGGATGAACACCTTCTTTAT
  GGTGACATAATCCGACAAGATTTTTTAGACACATATAATAACCTGACCTTGAAAACCATT
  ATGGCATTCAGGTGGGTAACTGAGTTTTGCCCCAATGCCAAGTACGTAATGAAGACAGAC
  ACTGATGTTTTCATCAATACTGGCAATTTAGTGAAGTATCTTTTAAACCTAAACCACTCA
  GAGAAGTTTTTCACAGGTTATCCTCTAATTGATAATTATTCCTATAGAGGATTTTACCAA
  AAAACCCATATTTCTTACCAGGAGTATCCTTTCAAGGTGTTCCCTCCATACTGCAGTGGG
  TTGGGTTATATAATGTCCAGAGATTTGGTGCCAAGGATCTATGAAATGATGGGTCACGTA
  AAACCCATCAAGTTTGAAGATGTTTATGTCGGGATCTGTTTGAATTTATTAAAAGTGAAC
  ATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATATAGAATCCATTTGGATGTCTGT
  CAACTGAGACGTGTGATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACTTTTTGG
  CAGGTCATGCTAAGGAACACCACATGCCATTATTAA
  
  Clone variation with respect to NM_003781.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003781 unedited
  ATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCTTTAATTTCATCTGG
  AATTCCACAGTTTTCTTAGCTCCCTGGACCCGGTTGACCTGTTGGCTCTTCCCGCTGGCT
  GCTCTATCACGTGGTGCTCTCCGACTACTCACCCCGAGTGTAAAGAACCTTCGGCTCGCG
  TGCTTCTGAGCTGCTGTGGATGGCCTCGGCTCTCTGGACTGTCCTTCCGAGTAGGATGTC
  ACTGAGATCCCTCAAATGGAGCCTCCTGCTGCTGTCACTCCTGAGTTTCTTTGTGATGTG
  GTACCTCAGCCTTCCCCACTACAATGTGATAGAACGCGTGAACTGGATGTACTTCTATGA
  GTATGAGCCGATTTACAGACAAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTC
  TCATCAAAATCCATTTCTGGTCATTCTGGTGACCTCCCACCCTTCAGATGTGAAAGCCAG
  GCAGGCCATTAGAGTTACTTGGGGTGAAAAAAAGTCTTGGTGGGGATATGAGGTTCTTAC
  ATTTTTCTTATTANGCCAAGAGGCTGAAAAGGAAGACAAAATGTTGGCATTGTCCTTAGA
  GGATGAACACCTTCTTTATGGTGACATAATCCGACAAGATTTTTTAGACACATATAATAA
  CCTGACCTTGAAAACCATTATGGCATTCAGGTGGGTAACTGAGTTNTGCCCCATGCCAAG
  TACGTAATGAAGACAGACACTGATGTTTTCATCAATACTGGNCATTTAGTGAAGTATCTT
  TTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGTTATCCTCTATTGNATATTATTCCT
  ATAGAGGATTTTCCAAAAACCCATATTTCTACCAGNAGTATCTTTCAGGGTGTCCCTCTA
  CTGCAGTGGGTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003781 unedited
  TTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTATAAAAAGGAAATACCCAAACCGGGGGCATTTATTGGCATTATCA
  GTATTACATACTGGACATAACTGAATGGGCTGTACTTTTATACAACTGGCAGTGTAATAG
  GTTTGTTTACACCAACATTTCCACAAACACGTGAGTAATGCACTAGGCTATGACATTAAA
  ACGGCTAAAACATCATGAGGCAATAAATTTTCTGCTCCATTATAATTTTATGGGACCACT
  ATTGTATATGTCATTATGCAGAACATAACTGTAATCTTCTGCAAAACCAGTCAAATTTAA
  CCATGCCACAAAAATAACTACCACCATTTGTCTTAAAAAACGTGACTCCTATATGGATGG
  AAATTAACAAGGGCATAAATCGATTCTATAAAATATTTCATTTAATTGATTTTATTATAA
  CTGGATTAGGTCTGAGCCCTGGGAAACAGACATCACCTTGTTATACAAAATCACACATTA
  TAGTTTTAACTGAAGTTGTTTTTCATATAGGACAATCATAAATATGAGTATTACAAGAGC
  TGACTACATACTTTAAAGTTTATATTTCCTGACAAAGGATATGACCCTTTTCCATCCTGT
  AATTAAAAAGAAAAAGACCACTTGCCCTATATAAACTCTAAAAGAAAACTGATAACACCC
  AGAACAGGGGGCAGGGAGTGCTCCCCTTTGACCTACTTTTACAGGTCCAAAAATGAAATT
  TACCCCGTCTTTGAAAAAATTTCTTCTATAAAAGCCCAAAATTAAAACGGGATTGACTTA
  ACTATTAAGGTTTCATCGTGATAGAAAATCTTAACAGCGCCGTTTAAATTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003781
• ORF Size: 996 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003781.2, NP_003772.1
• RefSeq Size: 3210
• RefSeq ORF: 996
• Locus ID: 8706
• Domains: Galactosyl_T
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - globo series, Metabolic pathways
• Gene Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) encodes isoform b. Variants 1-5 and 8-39 all encode the same isoform (b).