SPTLC1 (NM_006415) Human Untagged Clone

CAT#: SC111752

SPTLC1 (untagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPTLC1
• Synonyms: HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111752 sequence for NM_006415 edited (data generated by NextGen Sequencing)
  ATGGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCT
  CCTGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTC
  TCTAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTG
  ATTGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTC
  AACTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGT
  ATAAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCA
  GCTTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGC
  ACATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAA
  GCCATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAA
  AGAGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTTTGCTATTCAGAAAGGATTACAG
  GCATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTA
  CTAAAAGAACAAGAGATCGAAGATCAAAAGAATCCTCGCAAGGCTCGTGTAACTCGGCGT
  TTCATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAATTG
  GTTAAGTTAAAATACAAATACAAAGCAAGAATCTTCCTGGAGGAAAGCCTTTCATTTGGA
  GTCCTAGGAGAGCATGGCCGAGGAGTCACTGAACACTATGGAATCAATATTGATGATATT
  GATCTTATCAGTGCCAACATGGAGAATGCACTTGCTTCTATTGGAGGTTTCTGCTGTGGC
  AGGTCTTTTGTAATTGACCATCAGCGACTTTCCGGCCAGGGATACTGCTTTTCAGCTTCG
  TTACCTCCCCTGTTAGCTGCTGCAGCAATTGAGGCCCTCAACATCATGGAAGAGAATCCA
  GGTATTTTTGCAGTGTTGAAGGAAAAGTGCGGACAAATTCATAAAGCTTTACAAGGCATT
  TCTGGATTAAAAGTGGTGGGGGAGTCCCTTTCTCCAGCCTTTCACCTACAACTGGAAGAG
  AGCACTGGGTCTCGCGAGCAAGATGTCAGACTGCTTCAGGAAATTGTAGATCAATGCATG
  AACAGAAGTATTGCATTAACTCAGGCGCGCTACTTGGAGAAAGAAGAGAAGTGTCTCCCT
  CCTCCCAGCATTCGGGTTGTGGTCACGGTGGAACAAACAGAGGAAGAACTGGAGAGAGCT
  GCGTCCACCATCAAGGAGGTAGCCCAGGCCGTCCTGCTCTAG
  
  Clone variation with respect to NM_006415.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006415 unedited
  GGGTTCANAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTAACTAT
  GGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCTCC
  TGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTCTC
  TAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTGAT
  TGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTCAA
  CTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGTAT
  AAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCAGC
  TTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGCAC
  ATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAAGC
  CATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAAAG
  AGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTNTGCTATTCAGAAAGGATTACAGGC
  ATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTACT
  AAAAGAACCAAGAGATCGAGATCAAAAGAATCCTCGCAGGGCTCGTGTAACTCGGCGTTT
  CATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAAATTGG
  TAAGTTAAAATACANATTCAAAGCAAGAATCTTTCTGGNAGGAAAGNCCTTTCATTTGNN
  AGTCCCTAGAGAGCATGGNCCGAGNAGTCACTGNACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006415 unedited
  ACCGCGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGGAATTAGGTTTAT
  TTCATTAAAAAATAAAACAAAACAAACCAGACTCATCCCCCAAAGAGTACTTTATCCTTT
  AAAGCAGCAGCAATCACAAGGCACATATGTACAAAATACCTCAAGCAAAATAGAAACTGA
  ACATTCAAAAAGTATGTGTTGTTTAAAAAGATGTTGAAGACCATTTGAGTAATAAGCACA
  GGTTATAAAAATCACAATTTTATTCTTCTACCTTTAACAAGAGAAGGAATGATGGCATAA
  TATTTATATGCTATATTGTGGCACGTATAACAAACCTTTACACCACATAACCTGGTTTGC
  TAAAGAAAAAACAGGTAAAGTACTGCTTAAAAATGGTATTTTTAGTGCTTTCACATCTTT
  TGTAGATATCTGGAATTTGTAAGATGAAAAGAAAAATTCTCTTTTTCCTTGGCTTAGTAA
  AAGCCTGTAGTTACACGTCCCTAGGCAAACCCACCATACAAAAAAGGAATACATTTGAGA
  TATTCTAAAATATAAAGGATATGATTTATATTATGGGATCAACATAATAACCAACTCTCT
  TCCTAAAACAGATTAGAGGTGAACACATAAGAAATGTAAAGAAAGGTTAATTACATTAAA
  ATAAAATAGCTTCTGATTTAAATTATTGCTCACTTAGGAAGTATAATGAATATTCTCTGG
  CTAAGGATGCTCAGATTAGAAATGGTGTGACATCAATATTTAGTGTGTACAAAAAGTCTA
  AGATTAAATACATTGCANAGTTATAAATAATCACACAAAAGTGTCCTTTTAGCCACCATT
  TAGCTCACACAACGAAGACTGGAAAATACGTGGTTATTTAGATCTTCATATAAAATTGTA
  CACATATGTTGAAGTGTCCTCCTAGCTTTATGTTGCAGTCATTTTCTAATGAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006415
• ORF Size: 1422 bp
• Insert Size: 3010
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006415.2, NP_006406.1
• RefSeq Size: 2780
• RefSeq ORF: 1422
• Locus ID: 10558
• Domains: aminotran_1_2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript, and encodes isoform a.