CBFB (NM_001755) Human Untagged Clone

CAT#: SC111927

CBFB (untagged)-Human core-binding factor, beta subunit (CBFB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CBFB
• Synonyms: PEBP2B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001755, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCGCGTCGTGCCCGACCAGAGAAGCAAGTTCGAGAACGAGGAGTTTTTTAGGAAGCTGAGCCGCG
  AGTGTGAGATTAAGTACACGGGCTTCAGGGACCGGCCCCACGAGGAACGCCAGGCACGCTTCCAGAACGC
  CTGCCGCGACGGCCGCTCGGAAATCGCTTTTGTGGCCACAGGAACCAATCTGTCTCTCCAGTTTTTTCCG
  GCCAGCTGGCAGGGAGAACAGCGACAAACACCTAGCCGAGAGTATGTCGACTTAGAAAGAGAAGCAGGCA
  AGGTATATTTGAAGGCTCCCATGATTCTGAATGGAGTCTGTGTTATCTGGAAAGGCTGGATTGATCTCCA
  AAGACTGGATGGTATGGGCTGTCTGGAGTTTGATGAGGAGCGAGCCCAGCAGGAGGATGCATTAGCACAA
  CAGGCCTTTGAAGAGGCTCGGAGAAGGACACGCGAATTTGAAGATAGAGACAGGTCTCATCGGGAGGAAA
  TGGAGGTGAGAGTTTCACAGCTGCTGGCAGTAACTGGCAAGAAGACAACAAGACCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001755 unedited
  CGTCAGATTTGTACACGACTCATATGGGCGGCCGCGCAATTCGCACGAGGCGCGGGCGGG
  CGCCTGCAACAAAGGGAAGCGGGCGTCCGGGCGCCGCGGGTGGGCGGTCAGTCGGTCAGC
  GCGGAGCCAGCCAGCGGGTGCCCGCGCAAGCCCCGAGCGCGGCCGGCCGGCGCGGCCTCA
  GGGCGGGAAGATGCCGCGCGTCGTGCCCGACCAGAGAAGCAAGTTCGAGAACGAGGAGTT
  TTTTAGGAAGCTGAGCCGCGAGTGTGAGAGTAAGTACACGGGCTTCAGGGACCGGCCCCA
  CGAGGAACGCCAGGCACGCTTCCAGAACGCCTGCCGCGACGGCCGCTCGGAAATCGCTTT
  TGTGGCCACAGGAACCAATCTGTCTCTCCAGTTTTTTCCGGCCAGCTGGCAGGGAGAACA
  GCGACAAACACCTAGCCGAGAGTATGTCGACTTAGAAAGAGAAGCAGGCAAGGTATATTT
  GAAGGCTCCCATGATTCTGAATGGAGTCTGTGTTATCTGGAAAGGCTGGATTGATCTCCA
  AAGACTGGATGGTATGGGCTGTCTGGAGTTTGATGAGGAGCGAGCCCAGCAGGAGGATGC
  ATTAGCACAACAGGCCTTTGAAGAGGCTCGGAGAAGGACACGCGAATTTGAAGATAGAGA
  CAGGTCTCATCGGGAGGAAATGGAGGTGAGAGTTTCACAGCTGCTGGCAGTAACTGGCAA
  GAAGACAACAAGACCCTAGTCCTGGTTCCATTTACGGTGGTGGTGATGACCTCCAACTTC
  GTTCATTAATAGCACAGCAGAGGTGTGCTGCCCATCTTTACATACACATTGCTTCTAGTT
  GGCAGAGATAATGATTAACAGACCACAACTGTGATAACTGGAGGTACTACGGCTTATTTC
  TAACCTTGGCCGTCATAGACATCCCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001755 unedited
  TTGNTGCTACACAAAGCTTTCATTGTGGAAAGTAAATTAAACAAACAAACACACAGTATG
  GCCAATGGTGGCAAAAGCAATCTGGTAGCTTAAGGCAAGTTTTAATGGAGATGAATTTAG
  AAAAGATGATGCAATGATGTAATTTTGTTTAAATATAGATTATGATAAAAATATCAAATT
  GCATCAATAACTTAATAGAAGTAGTTTTCAAAGCCTGTACTACTGGAGAAAAAGGGCTCA
  TGATCTTAATGCCAGCATTAAGACAGACAGAAGCAGCATGGTGCTAAATGACAGAAAAAT
  CCAGTATTTACATTAGGATGGTAACAGGAACATTTAAGAGCATTTGATCTTATTTACTGG
  TGGACCGAAAGCCCTTAGCTAGACAATAGAGGAAAATGTACCATCATCATGTTATTTAAA
  AAAATTTAAGTGCAGAAGTAAAAAACCAAGGAGCTCTGTAATAAAGTAACATAAAACACC
  ACTGTAAGGATCACTTAAAAAGCGATTCCCTCAGGGGAAATTAGGGCACTGAGTGTGTAA
  ACATGTGAGCTGTACAACACCAAAACCAACCAAATTCATTCATAGTACTTATAGTTATGC
  AAGATCAGCATGGCCTTTAATGACGCATTATTGTTTGTAACAATTTAAACACACTCCTTT
  CCATGCATAACTTCAAATATATAACAAATACTTTTATTTGATACAGGATATCTGTTTATG
  AAATAAGACCTATATACAATGAAAGTAACACCTCCCTCATCAGTCAAGTACAGTTATATG
  CTTTTTAAATGCAATGACCTTATTCAGACTTCAGTCCATTTTCTGAATTGACTGTGTCAG
  TTTTTCACCCTTAAAATAATGCATCTAAGATAAAATACATTTTCAAAATCCAGAATGTAG
  AACATTTATAAATGTGACCTGAAAATTTTGTAAGGAGTGCCAATGACTTACACTGTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001755
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001755.2, NP_001746.1
• RefSeq Size: 3181 bp
• RefSeq ORF: 549 bp
• Locus ID: 865
• Cytogenetics: 16q22.1
• Domains: CBF_beta
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region compared to variant 1, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.