Cyclophilin A (PPIA) (NM_021130) Human Untagged Clone

CAT#: SC111937

PPIA (untagged)-Human peptidylprolyl isomerase A (cyclophilin A) (PPIA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPIA
• Synonyms: CYPA; CYPH; HEL-S-69p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111937 sequence for NM_021130 edited (data generated by NextGen Sequencing)
  ATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCGACGGCGAGCCCTTGGGCCGCGTC
  TCCTTTGAGCTGTTTGCAGACAAGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGC
  ACTGGAGAGAAAGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT
  ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGG
  GAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCTTGTCCATG
  GCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCTGCACTGCCAAGACTGAG
  TGGTTGGATGGCAAGCATGTGGTGTTTGGCAAAGTGAAAGAAGGCATGAATATTGTGGAG
  GCCATGGAGCGCTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAGATCACCATTGCTGAC
  TGTGGACAACTCGAATAA
  
  Clone variation with respect to NM_021130.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021130 unedited
  CGACTCACATAGGCGGCCGCGAAATTCGCACGAGGTGCCACCGCCGAGGAAAAACCGTGT
  ACTCTTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCGACGGCGAAGCCCT
  TGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCCCAAAGACAGCAGAAAAATTTT
  CGTGCTCTGAGCACTGGAGAGAAAGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATT
  ATTCCAGGGTTTATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAG
  TCCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAANAGCATACGGGTCCTGG
  CATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCTGCAC
  TGCCAAGACTGAGTGGTTGGATGGCAAGCATGTGGGTGTTTGGCAAAGTGAAAGAAGCAT
  GAATATTGTTGGAGGCCATGGAGCGCTTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAG
  ATCACCATTGNCTGACTGTGGACACTGGAATAAGTTTGACTTTGGTGTTTTATCTTAACC
  ACCAGATCATTCCTTCTGTAGCTCAGGAGAGCACCCCTCCACCCCATTTGCTTCGCAGTA
  TCCTAGAATACTTTGTGCTCTCGCCTGCAGTCCCCTCTTTGGGTTTCCAATGGTTTTCCT
  TTGGTCCCTTCCATGCCTAGNCTTGATTGCAAAGGNTTAAGTTTATTGATTATGAAAATA
  AAACTAANNTACANTGTCCTCGTTTGAGTTTAAAAAGAGTGGGAATGTAGGCTTTAATTT
  AGCAGAAATGGGGGTTACTTCTGAAACATNACTTGGTTTGCTTAATTCTAACAGACTTAT
  AAAATTTTTTTACCTTTCATCCCCAGAAAGGTAAATAGTTTTTGTTGGGGGGGAAAATTT
  AAAAAAGTAGGACCAACTGGGCATGGGGGGCTACTGGCTTTGAAATATATACCTGAGCAA
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021130 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAATGAAAAGAGTTTGATGTTTAT
  TTCCACCTTGCACTCAGGTCTGAGCCACAAGTACATTAAGACATTGAATGGTATCACCCA
  GGGAATACGTAACCAGACAACACACAAGACTGAGATGCACAAGTGGTGGTGGTGGTAATT
  CACGCAGAAGGAACCAGACAGTAAAACAAAAATTGCCCAACACACCAAATGATCAAATCC
  GCCACCTCTAGGATAGGCAAACTTGATTGCTGGGTTAAGAACCCTATAGGTCTGTTAAGG
  TGGGCAGAGAAGGGGTTTTCTCAGCTTATATTGTCCTGACATCTAACTGCCAGCAAGCAC
  TGTACATATAATTTCCTGAGAAACCAAGTCCTTAGTGGGAAGGGTATCCCTTTGACCAGA
  TCTTATGGCTTAAATTGGTCAGGTTTGCAAAACCTCAAAGCCTCCATAACCAAAGCTAGG
  GAGAGGCTCTATATGCTACAAGCAGTACCTCCTCACTGCAGGTAGTCTGCGCCTTAACCC
  TCTGCAGGGAGACTGACTGTAGCACCAAGTACCTGGCTTTTTAGACTCTACATAGGAATT
  CCACCATAATTAAGATGTATAAACTTGACCTACAGCCTATAGCCCAATATGCTTCCCTCA
  AGACATCAACAGTGTTCAAGCTTATCTTTGTTACAGAAAATGATGTAGGGTGCGGTGGCT
  CACATCTGTAATCTCANCAGTTTGGGAGGCCAAGGCAGGCGCATCAGCTGAGGTCAGAAG
  TTGAGACAGNCCTGTCAAATGATGAAACCCATCTTACTAACATACAAAAATAGCTGGGCA
  TGGTGCACACGCNCGTTATCCAGCTACTCAGAGCTGAGCACCAGATCACTTGATCCAGNA
  GCGGAGCTGCNTGANCTAAATACGCCATGCTTCAGGNATAAAATAAAAGAACGACAGAGT
  CTATTTTGAATAGCATCTGAAACACTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021130
• Insert Size: 2290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021130.2, NP_066953.1
• RefSeq Size: 1652 bp
• RefSeq ORF: 498 bp
• Locus ID: 5478
• Cytogenetics: 7p13
• Domains: pro_isomerase
• Gene Summary: 'This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.