Cyclophilin A (PPIA) (NM_021130) Human Untagged Clone

CAT#: SC111937

PPIA (untagged)-Human peptidylprolyl isomerase A (cyclophilin A) (PPIA)


  "NM_021130" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPIA
Synonyms CYPA; CYPH; HEL-S-69p
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111937 sequence for NM_021130 edited (data generated by NextGen Sequencing)
ATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCGACGGCGAGCCCTTGGGCCGCGTC
TCCTTTGAGCTGTTTGCAGACAAGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGC
ACTGGAGAGAAAGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAGGGTTT
ATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAGTCCATCTATGGG
GAGAAATTTGAAGATGAGAACTTCATCCTAAAGCATACGGGTCCTGGCATCTTGTCCATG
GCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCTGCACTGCCAAGACTGAG
TGGTTGGATGGCAAGCATGTGGTGTTTGGCAAAGTGAAAGAAGGCATGAATATTGTGGAG
GCCATGGAGCGCTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAGATCACCATTGCTGAC
TGTGGACAACTCGAATAA

Clone variation with respect to NM_021130.3
>OriGene 5' read for NM_021130 unedited
CGACTCACATAGGCGGCCGCGAAATTCGCACGAGGTGCCACCGCCGAGGAAAAACCGTGT
ACTCTTAGCCATGGTCAACCCCACCGTGTTCTTCGACATTGCCGTCGACGGCGAAGCCCT
TGGGCCGCGTCTCCTTTGAGCTGTTTGCAGACAAGGTCCCAAAGACAGCAGAAAAATTTT
CGTGCTCTGAGCACTGGAGAGAAAGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATT
ATTCCAGGGTTTATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGCAAG
TCCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAANAGCATACGGGTCCTGG
CATCTTGTCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCTGCAC
TGCCAAGACTGAGTGGTTGGATGGCAAGCATGTGGGTGTTTGGCAAAGTGAAAGAAGCAT
GAATATTGTTGGAGGCCATGGAGCGCTTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAG
ATCACCATTGNCTGACTGTGGACACTGGAATAAGTTTGACTTTGGTGTTTTATCTTAACC
ACCAGATCATTCCTTCTGTAGCTCAGGAGAGCACCCCTCCACCCCATTTGCTTCGCAGTA
TCCTAGAATACTTTGTGCTCTCGCCTGCAGTCCCCTCTTTGGGTTTCCAATGGTTTTCCT
TTGGTCCCTTCCATGCCTAGNCTTGATTGCAAAGGNTTAAGTTTATTGATTATGAAAATA
AAACTAANNTACANTGTCCTCGTTTGAGTTTAAAAAGAGTGGGAATGTAGGCTTTAATTT
AGCAGAAATGGGGGTTACTTCTGAAACATNACTTGGTTTGCTTAATTCTAACAGACTTAT
AAAATTTTTTTACCTTTCATCCCCAGAAAGGTAAATAGTTTTTGTTGGGGGGGAAAATTT
AAAAAAGTAGGACCAACTGGGCATGGGGGGCTACTGGCTTTGAAATATATACCTGAGCAA
A
>OriGene 3' read for NM_021130 unedited
CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAATGAAAAGAGTTTGATGTTTAT
TTCCACCTTGCACTCAGGTCTGAGCCACAAGTACATTAAGACATTGAATGGTATCACCCA
GGGAATACGTAACCAGACAACACACAAGACTGAGATGCACAAGTGGTGGTGGTGGTAATT
CACGCAGAAGGAACCAGACAGTAAAACAAAAATTGCCCAACACACCAAATGATCAAATCC
GCCACCTCTAGGATAGGCAAACTTGATTGCTGGGTTAAGAACCCTATAGGTCTGTTAAGG
TGGGCAGAGAAGGGGTTTTCTCAGCTTATATTGTCCTGACATCTAACTGCCAGCAAGCAC
TGTACATATAATTTCCTGAGAAACCAAGTCCTTAGTGGGAAGGGTATCCCTTTGACCAGA
TCTTATGGCTTAAATTGGTCAGGTTTGCAAAACCTCAAAGCCTCCATAACCAAAGCTAGG
GAGAGGCTCTATATGCTACAAGCAGTACCTCCTCACTGCAGGTAGTCTGCGCCTTAACCC
TCTGCAGGGAGACTGACTGTAGCACCAAGTACCTGGCTTTTTAGACTCTACATAGGAATT
CCACCATAATTAAGATGTATAAACTTGACCTACAGCCTATAGCCCAATATGCTTCCCTCA
AGACATCAACAGTGTTCAAGCTTATCTTTGTTACAGAAAATGATGTAGGGTGCGGTGGCT
CACATCTGTAATCTCANCAGTTTGGGAGGCCAAGGCAGGCGCATCAGCTGAGGTCAGAAG
TTGAGACAGNCCTGTCAAATGATGAAACCCATCTTACTAACATACAAAAATAGCTGGGCA
TGGTGCACACGCNCGTTATCCAGCTACTCAGAGCTGAGCACCAGATCACTTGATCCAGNA
GCGGAGCTGCNTGANCTAAATACGCCATGCTTCAGGNATAAAATAAAAGAACGACAGAGT
CTATTTTGAATAGCATCTGAAACACTTAT
Restriction Sites NotI-NotI     
ACCN NM_021130
Insert Size 2290 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021130.2, NP_066953.1
RefSeq Size 1652 bp
RefSeq ORF 498 bp
Locus ID 5478
Cytogenetics 7p13
Domains pro_isomerase
Gene Summary 'This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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