DENND1A (NM_024820) Human Untagged Clone

CAT#: SC111995

DENND1A (untagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DENND1A
• Synonyms: FAM31A; KIAA1608
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_024820 edited
  ATGGGCTCCAGGATCAAGCAGAATCCAGAGACCACATTTGAAGTATATGTTGAAGTGGCC
  TATCCCAGGACAGGTGGCACTCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGAC
  TACAGTGACCAGGAAGTTCTACAGACTTTGACCAAGTTTTGTTTCCCCTTCTATGTGGAC
  AGCCTCACAGTTAGCCAAGTTGGCCAGAACTTCACATTCGTGCTCACTGACATTGACAGC
  AAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAGCGAAGAGCTGCTTCTGTATCTTA
  AGCT:ATCTCCCCTGGTTCGAGGTATTTTATAAGCTGCTTAACATCCTGGCAGATTACAC
  GACAAAAAGACAGGAAAATCAGTGGAATGAGCTTCTTGAAACTCTGCACAAACTTCCCAT
  CCCTGACCCAGGAGTGTCTGTCCATCTCAGCGTGCATTCTTATTTTACTGTGCCTGATAC
  CAGAGAACTTCCCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGA
  TGTTAACAACATGTTGCATCTGTACGCCAGTATGCTGTACGAACGCCGGATACTCATCAT
  TTGCAGCAAACTCAGCACTCTGACTGCCTGCATCCACGGGTCTGCGGCGATGCTCTACCC
  CATGTACTGGCAGCACGTGTACATCCCCGTGCTGCCGCCGCATCTGCTGGACTACTGCTG
  TGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAAAGTCAGAAACAT
  GGCCCTGGATGATGTCGTGATCCTGAATGTGGACACCAACACCCTGGAAACCCCCTTCGA
  TGACCTCCAGAGCCTCCCAAACGACGTGATCTCTTCCCTGAAGAACAGGCTGAAAAAGGT
  CTCCACAACCACTGGGGATGGTGTGGCCAGAGCGTTCCTCAAGGCCCAGGCTGCTTTCTT
  CGGTAGCTACCGAAACGCTCTGAAAATCGAGCCGGAGGAGCCGATCACTTTCTGTGAGGA
  AGCCTTCGTGTCCCACTACCGCTCCGGAGCCATGAGGCAGTTCCTGCAGAACGCCACACA
  GCTGCAGCTCTTCAAGCAGTTTATTGATGGTCGATTAGATCTTCTCAATTCCGGCGAAGG
  TTTCAGTGATGTTTTTGAAGAGGAAATCAACATGGGCGAGTACGCTGGCAGTGACAAACT
  GTACCATCAGTGGCTCTCCACTGTCCGGAAAGGAAGTGGAGCAATTCTGAATACTGTAAA
  GACCAAAGCAAATCCGGCCATGAAGACTGTCTACAAGTTCGCAAAAGATCATGCAAAAAT
  GGGAATAAAAGAGGTGAAAAACCGCTTGAAGCAAAAGGACATTGCCGAGAATGGCTGCGC
  CCCCACCCCAGAAGAGCAGCTGCCAAAGACTGCACCGTCCCCACTGGTGGAGGCCAAGGA
  CCCCAAGCTCCGAGAAGACCGGCGGCCAATCACAGTCCACTTTGGACAGGTGCGCCCACC
  TCGTCCACATGTTGTTAAGAGACCAAAGAGCAACATCGCAGTGGAAGGCCGGAGGACGTC
  TGTGCCGAGCCCTGAGCAAAACACCATTGCAACACCAGCTACACTCCACATCCTACAGAA
  AAGCATTACCCATTTTGCGGCCAAGTTCCCGACGAGAGGCTGGACCTCTTCATCACATTG
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024820
• ORF Size: 1680 bp
• Insert Size: 1700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_024820.2.

Reference Data

• RefSeq: NM_024820.2, NP_079096.2
• RefSeq Size: 2166
• RefSeq ORF: 1680
• Locus ID: 57706
• Domains: DENN, dDENN, uDENN
• Gene Summary: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]). [supplied by OMIM, Nov 2010]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.