PHF1 (NM_024165) Human Untagged Clone

CAT#: SC112257

PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHF1
• Synonyms: hPHF1; MTF2L2; PCL1; PHF2; TDRD19C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112257 sequence for NM_024165 edited (data generated by NextGen Sequencing)
  ATGGCGCAGCCCCCCCGGCTGAGCCGCTCTGGTGCCTCCTCACTTTGGGACCCAGCTTCT
  CCTGCTCCCACCTCTGGCCCCAGGCCTCGGCTTTGGGAGGGTCAAGATGTGCTGGCCAGA
  TGGACTGATGGGCTGCTATACTTGGGTACCATCAAAAAGGTGGACAGTGCTAGGGAGGTG
  TGTCTGGTCCAGTTTGAGGATGATTCGCAGTTTCTGGTTCTATGGAAAGACATTAGCCCT
  GCTGCCCTCCCTGGAGAGGAACTCCTCTGTTGTGTCTGTCGCTCTGAGACTGTGGTCCCT
  GGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGACTGCCATGTT
  CCCAGGGCTCCAGCCCCTGGAGAGGGAGAGGGCACATCCTGGGTATGCCGCCAGTGTGTC
  TTTGCGATCGCCACCAAGAGGGGAGGTGCCCTGAAGAAGGGCCCCTATGCCCGGGCCATG
  CTGGGTATGAAGCTTTCTCTGCCATATGGACTGAAGGGGCTGGACTGGGATGCTGGACAT
  CTGAGCAACCGACAGCAGAGTTACTGTTACTGTGGTGGCCCTGGGGAGTGGAACCTGAAA
  ATGCTGCAGTGCCGGAGCTGCCTGCAGTGGTTCCATGAGGCCTGCACCCAGTGTCTGAGC
  AAGCCCCTCCTCTATGGGGACAGGTTCTATGAATTTGAATGCTGTGTGTGTCGCGGGGGC
  CCTGAGAAAGTCCGGAGACTACAGCTTCGCTGGGTGGATGTGGCCCATCTTGTCCTGTAT
  CACCTCAGTGTTTGCTGTAAGAAGAAATACTTTGATTTTGATCGTGAGATCCTCCCCTTC
  ACTTCTGAGAATTGGGACAGTTTGCTCCTGGGGGAGCTTTCAGACACCCCCAAAGGAGAA
  CGTTCTTCCAAGCTCCTCTCTGCTCTTAACAGCCACAAGGACCGTTTCATTTCAGGGAGA
  GAGATTAAGAAGAGGAAATGTTTGTTTGGTCTCCATGCTCGGATGCCTCCCCCTGTGGAG
  CCCCCTACTGGAGATGGAGCACTCACCAGCTTCCCTTCAGGGCAGGGCCCTGGGGGAGGG
  GTCTCACGTCCCCTGGGGAAGCGCCGGAGGCCGGAGCCAGAGCCCCTGAGGAGGAGGCAG
  AAGGGGAAAGTGGAGGAGCTGGGGCCACCCTCAGCAGTGCGCAATCAGCCCGAGCCCCAG
  GAGCAGAGGGAGCGGGCTCATCTGCAGAGGGCACTGCAGGCCTCAGTGTCTCCACCATCC
  CCCAGCCCTAACCAGAGTTACCAGGGCAGCAGCGGCTACAACTTCCGGCCCACAGATGCC
  CGCTGCCTGCCCAGCAGCCCCATCCGGATGTTTGCTTCCTTCCACCCTTCTGCCAGCACC
  GCAGGGACCTCTGGGGACAGTGGACCCCCAGACAGGTCACCCCTGGAACTTCACATTGGT
  TTCCCCACAGACATCCCTAAAAGTGCCCCCCACTCGATGACTGCCTCATCTTCCTCAGTT
  TCATCCCCATCCCCAGGTCTTCCTAGACGCTCAGCACCCCCTTCTCCCCTGTGCCGTAGT
  TTGTCTCCTGGGACTGGGGGAGGAGTCCGAGGTGGGGTTGGTTACCTGTCCCGAGGGGAC
  CCTGTCCGGGTCCTTGCTCGGAGAGTACGGCCTGATGGCTCTGTGCAGTACCTGGTTGAG
  TGGGGAGGAGGGGGCATCTTCTGA
  
  Clone variation with respect to NM_024165.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024165 unedited
  AAACGTCTTCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCTGC
  TGCGTCATACGCCCCAAAGCCGCCGGGACGGAGGGGCTGGGCCTGGGGACCCCCCGGCCT
  CCGCCTGCACGCCCCCCCACGCCCGGACGTGCCCTCTCCGCGCGGGGGACTCGCCTAGGT
  CTCCTACGTCTGCCCCTGCCCGGCTCCCGGCGGCCCCAGCTGTCACCGGCCCCCCCAGGA
  TGCAATGGCGCAGCCCCCCCGGCTGAGCCGCTCTGGTGCCTCCTCACTTTGGGACCCAGC
  TTCTCCTGCTCCCACCTCTGGCCCCAGGCCTCGGCTTTGGGAGGGTCAAGATGTGCTGGC
  CAGATGGACTGATGGGCTGCTATACTTGGGTACCATCAAAAAGGTGGACAGTGCTAGGGA
  GGTGTGTCTGGTCCAGTTTGAGGATGATTCGCAGTTTCTGGTTCTATGGAAAGACATTAG
  CCCTGCTGCCCTCCCTGGAGAGGAACTCCTCTGTTGTGTCTGTCGCTCTGAGACTGTGGT
  CCCTGGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGACTGCCA
  TGTTCCCAGGGCTCCACCCCCTTGGAAGAGGGAGAGGGCACATCCTGGGGATGCCGCCAG
  TGTGTCTTTGCGATCGCCACCCAGAGGGGGAGTGCCCTGNAGAAGGGCCCCTATGCCCGG
  GCCATGCTGGGTATGAAAGCTTTCTCTGCCATATGGACTGAAAGGGCTGGACTGGGATGC
  TTGACATCTGAGCAACCGACAGCAGAGTTACTGTTACTGGGGTGGCCCTGGGGAGTGGGA
  CCTGAAAAGCTGCAATGCCCGGACTGCCTGCANTGGTTTCATGGAGCCCTGACCCCATGG
  CC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024165 unedited
  NAAGCTGCATCATGGTACTCTGCCGCCTCNANGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTGTTTATTTCTCTTTATTTCAATCATACAGAGTCCGGGATCCCTTATCAT
  CCTCCCTCCTCAGTCTCTGCCCCCAGGGGTTAAATAATTTTAAAAAATATTTAAAAAGCT
  GTAACAAAATAACCTCAAAGGAAATGGGAAGGATGGGAGGGGTCAAGAATCATGAAGGAG
  GGTAGAGAAGAGGGCCTCTTCCCCACTCCCCACTCTTGGATTCCAGCCTGGGCAGTAGGG
  AGAACTACCCCCTATCTCTCCAGGTACATCCCAACTGTAGGTGAAGTCAAAAGTCAAGGT
  ATGAAAGTGCCGGTGTGTGTGAATGGGGAGCTGGGCAAAAGCAGGCTGTTCAAAAAATGC
  CCCCTCCTCCCCACTCAACCAGGTACTGGACAGAGCCATCAAGCCGTACTCTCCGAGCAA
  GGACCCGGACAGGGTCCCCTCGGGACAGGTAACCAACCCCACCTCGGACTCCTCCCCCAG
  TCCCAGGAGACAAACTCGGGCCCAAGGGGATTATTGGGGTGCCTGAGCGTCTAGGAAGAC
  CTGGGGATGGGGATGAAACTCGAGAAGATGAGGCAGTCATCGAGTGGGGGGCACTTTTAA
  GGATGTCTGTGGGGAAACCAATGTGAAGTTCCAGGGGGTGACCTGTCTGGGGGTCCACTG
  TTCCCCAGAGTCCCTGCGGTGCTTGCAAAAAGGTGGGAAGGAGCCAACATCCGGATGGGC
  CTGCTTGGCAAGGCACGGGCATTTGTGGGCCCGAAATTGTTACCGCTGCTGCCCCGGTAA
  CTCTGGGTAGGCTGGGGATTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024165
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024165.1, NP_077084.1
• RefSeq Size: 2260 bp
• RefSeq ORF: 1704 bp
• Locus ID: 5252
• Cytogenetics: 6p21.32
• Domains: PHD, TUDOR
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]'
  Transcript Variant: This variant (2), also called variant PHF2, represents the longest transcript and encodes the longer isoform (b).