Epoxide hydrolase (EPHX1) (NM_000120) Human Untagged Clone

CAT#: SC112331

EPHX1 (untagged)-Human epoxide hydrolase 1, microsomal (xenobiotic) (EPHX1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPHX1
• Synonyms: EPHX; EPOX; HYL1; MEH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112331 sequence for NM_000120 edited (data generated by NextGen Sequencing)
  ATGTGGCTAGAAATCCTCCTCACTTCAGTGCTGGGCTTTGCCATCTACTGGTTCATCTCC
  CGGGACAAAGAGGAAACTTTGCCACTTGAAGATGGGTGGTGGGGGCCAGGCACGAGGTCC
  GCAGCCAGGGAGGACGACAGCATCCGCCCTTTCAAGGTGGAAACGTCAGATGAGGAGATC
  CACGACTTACACCAGAGGATCGATAAGTTCCGTTTCACCCCACCTTTGGAGGACAGCTGC
  TTCCACTATGGCTTCAACTCCAACTACCTGAAGAAAGTCATCTCCTACTGGCGGAATGAA
  TTTGACTGGAAGAAGCAGGTGGAGATTCTCAACAGATACCCTCACTTCAAGACTAAAATT
  GAAGGGCTGGACATCCACTTCATCCACGTGAAGCCCCCCCAGCTGCCCGCAGGCCATACC
  CCGAAGCCCTTGCTGATGGTGCACGGCTGGCCCGGCTCTTTCTACGAGTTTTATAAGATC
  ATCCCACTCCTGACTGACCCCAAGAACCATGGCCTGAGCGATGAGCACGTTTTTGAAGTC
  ATCTGCCCTTCCATCCCTGGCTATGGCTTCTCAGAGGCATCCTCCAAGAAGGGGTTCAAC
  TCGGTGGCCACCGCCAGGATCTTTTACAAGCTGATGCTGCGGCTGGGCTTCCAGGAATTC
  TACATTCAAGGAGGGGACTGGGGGTCCCTGATCTGCACTAATATGGCCCAGCTGGTGCCC
  AGCCACGTGAAAGGCCTGCACTTGAACATGGCTTTGGTTTTAAGCAACTTCTCTACCCTG
  ACCCTCCTCCTGGGACAGCGTTTCGGGAGGTTTCTTGGCCTCACTGAGAGGGATGTGGAG
  CTGCTGTACCCCGTCAAGGAGAAGGTATTCTACAGCCTGATGAGGGAGAGCGGCTACATG
  CACATCCAGTGCACCAAGCCTGACACCGTAGGCTCTGCTCTGAATGACTCTCCTGTGGGT
  CTGGCTGCCTATATTCTAGAGAAGTTTTCTACCTGGACCAATACGGAATTCCGATACCTG
  GAGGATGGAGGCCTGGAAAGGAAGTTCTCCCTGGACGACCTGCTGACCAACGTCATGCTC
  TACTGGACAACAGGCACCATCATCTCCTCCCAGCGCTTCTACAAGGAGAACCTGGGACAG
  GGCTGGATGACCCAGAAGCATGAGCGGATGAAGGTCTATGTGCCCACTGGCTTCTCTGCC
  TTCCCTTTTGAGCTATTGCACACGCCTGAAAAGTGGGTGAGGTTCAAGTACCCAAAGCTC
  ATCTCCTATTCCTACATGGTTCGTGGGGGCCACTTTGCGGCCTTTGAGGAGCCGGAGCTG
  CTCGCCCAGGACATCCGCAAGTTCCTGTCGGTGCTGGAGCGGCAATGA
  
  Clone variation with respect to NM_000120.3
  357 g=>a;990 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000120 unedited
  GTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGCAGAGCTCCC
  AGCTCTCTTTCCCAAGGAGTAATCAGAGGGTGAGAACGTGGAGCCTGGTGGACAGGTGAA
  AGCACTGGGATCTTTCTGCCCAGAAAGGGGAAAGTTGCACATTTATATCCTAGAGGGAAG
  CGACAGCAGTGCTTCTCCCTGTGCTGAGGAGCCATGTGGCTAGAAATCCTCCTCACTTCA
  GTGCTGGGCTTTGCCATCTACTGGTTCATCTCCCGGGACAAAGAGGAAACTTTGCCACTT
  GAAGATGGGTGGTGGGGGCCAGGCACGAGGTCCGCAGCCAGGGAGGACGACAGCATCCGC
  CCTTTCAAGGTGGAAACGTCAGATGAGGAGATCCACGACTTACACCAGAGGATCGATAAG
  TTCCGTTTCACCCCACCTTTGGAGGACAGCTGCTTCCACTATGGCTTCAACTCCAACTAC
  CTGAAGAAAGTCATCTCCTACTGGCGGAATGAATTTGACTGGAAGAAGCAGGTGGAGATT
  CTCAACAGATACCCTCACTTCAAGACTAAAATTGAAGGGCTGGACATCCACTTCATCCAC
  GTGAAGCCCCCCCAGCTGCCCGCAGGCCATACCCCGAAGCCCTTGCTGATGGTGCACGGC
  TGGCCCGGCTCTTTCTACGAGTTTTATAAGATCATCCCACTCCTGACTGACCCCAAGAAC
  CATGGCCTGAGCGATGAGCACGTTTTTGAAGTCATCTGCCCTTCCATCCCTGGCTATGGC
  TTCTCAGAGGCATCCTCCAGAAGGNGTTCAACTCGGTGGCCACGCCAGGATCTTTTAAAG
  CTGATGCTGNCGCTGNGCTTCCAGGAATTCTACATTCAGGGGAGGGACTGGGGTCCCTGA
  TCTGCACTATATGGCCCNAGCTGTGCCCANCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000120 unedited
  NTTCTTGNACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTAGAGTAAAG
  TCGTTTATCATCAAAGCCATGTTGCTTACCACACGGAGTTGGGGGTTGGGGAGTGAGCTT
  GGAGGGGTGAGGGGGTGAGCGTGGGCTCCCAGCATGGGCAGGGGACGGAGGCAAACTCAT
  TCCTCAGAAAAGGGGTATCTTCCCCAAGAAAAGCCTGGAGGGCACTTGTGGGGGGAGGTG
  GCAGGCGGGGGGAGAGGGGTGGGTCATTGCCGCTCCAGCACCGACAGGAACTTGCGGATG
  TCCTGGGCGAGCAGCTCCGGCTCCTCAAAGGCCGCAAAGTGGCCCCCACGAACCATGTAG
  GAATAGGAGATGAGCTTTGGGTACTTGAACCTCACCCACTTTTCAGGCGTGTGCAATAGC
  TCAAAAGGGAAGGCAGAGAAGCCAGTGGGCACATAGACCTTCATCCGCTCATGCTTCTGG
  GTCATCCAGCCCTGTCCCAGGTTCTCCTTGTAAAAGCGCTGGGAGGAGATGATGGTGCCT
  GTTGTCCAGTAGAGCATGACGTTGGTCAGCAGGTCGTCCAGGGAGAACTTCCTTTCCAGG
  CCCTCATCCTTCAGGTATCGGAATTCCGTATTGGTCCAGGTAGAAAACTTCTCTAGAATA
  TAGGCAGCCGGACCCACAGAGAGTCATTCAGAGCAGAACCTACCGTGTCAGGCTTGGTGC
  ACTGGATGTGCATGTAACCGCTTTTCCTTATAAGGCTGTAAAATACCTCTTCTTTGACGG
  GTACAGAAGTTCCCATTCCTTTTAATGAGGCCAAGAACCTTCCGAAACCCTGTCCCAGGA
  GAAGGGTCAGGGTAAAAAATTTTGTTAAAACCAAAGCCTGTTCAGGGCCGGCCCTTACGG
  GGTTGGGCCCACATGGGCCTATTATGGAATACAGGACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000120
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000120.2, NP_000111.1
• RefSeq Size: 1856 bp
• RefSeq ORF: 1368 bp
• Locus ID: 2052
• Cytogenetics: 1q42.12
• Domains: abhydrolase
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (1) is the longer transcript. Variants 1, 2 and 3 encode the same protein.