SNRPN (NM_022808) Human Untagged Clone

CAT#: SC112434

SNRPN (untagged)-Human small nuclear ribonucleoprotein polypeptide N (SNRPN), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNRPN
• Synonyms: HCERN3; PWCR; PWS; RT-LI; SM-D; sm-N; SMN; SNRNP-N; SNURF-SNRPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022808, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTGTTGGCAAGAGTAGCAAGATGCTGCAGCACATTGACTATAGAATGAGATGTATCCTGCAAGATG
  GCCGAATCTTCATTGGCACCTTTAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGA
  GTTCAGAAAGATCAAGCCAAAGAATGCGAAGCAACCAGAGCGTGAAGAAAAGCGGGTTTTGGGTCTGGTG
  TTGCTGCGTGGGGAGAACTTGGTATCCATGACTGTGGAGGGGCCACCCCCCAAAGATACTGGCATTGCTC
  GGGTACCACTTGCTGGAGCTGCTGGAGGCCCTGGGGTTGGTAGGGCAGCTGGTAGAGGAGTACCAGCTGG
  TGTGCCAATTCCCCAGGCCCCTGCTGGATTGGCAGGCCCTGTCCGAGGAGTTGGGGGACCATCCCAGCAG
  GTAATGACTCCACAGGGAAGAGGCACTGTAGCAGCTGCTGCTGTTGCTGCGACTGCCAGTATTGCTGGAG
  CCCCAACACAGTACCCACCAGGACGGGGCACTCCGCCCCCACCCGTCGGCAGAGCAACCCCACCTCCAGG
  CATTATGGCTCCTCCACCTGGTATGAGACCACCCATGGGCCCACCAATTGGGCTTCCCCCTGCTCGAGGG
  ACGCCAATAGGCATGCCGCCTCCGGGAATGAGACCCCCTCCACCAGGCATTAGAGGTCCACCTCCCCCAG
  GAATGCGTCCACCAAGACCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022808 unedited
  GTATTTGTATACGACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGGAGCCTTCTGTG
  TGGTTTGCGGGGGCAAGGTCAGCTGCCCCCTGCCCTCTCTCTGGGGCTATTTGAGGAATG
  CGGCCTTTTTGCATAAGAAAGGCTTTTCTCTACAGTAACTGTGGTCGCTGATCAATGACA
  CCTTCCTGATGTAAGTGGGATCTGTGGATCTGAAAATTCACAGTGGCCAGCTCACCACCA
  CCTGATGAAAGATACACCACAGGGTGAGAGCATCCTAACAGCAAACTGCAAATGAGATAC
  TTTGACGAAGGTAATTGGGACTCCCATCAAGTCTCCACACAGCTAGCAGCCACGTGGGGC
  ACTTCTCCAGCATATGTAAGTGGAACTCAGAAGGCAACATTCCCTGAACATACTCTTCCA
  CCAGAATCTCCTCTACAGATTTTTGCTGCCCCTTTTACCAGTGGCTGAATCTACTTTCTC
  TTATGGATCGCTTACACCTGAGACGAACTACAGAACAGCACGTACCAGAGGTGGAAGTCC
  AAGTCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAGTTGTACCCGAGGC
  GTTCTCAGCAGCAGCAAGTACCTGTGGTGGATNNTCCAGCTGAACTGAGGCANGCATTTC
  TAGCTGAGACACCAAGAAGTGGTTAAAGCCATATTGGAGTAGCGAGGAATCTGATTCCAA
  GCAAAACCCAGCTCCATCTACTCTTTGAAGCTTCTGCCCAGCTTGCATTGTTTCTAAGAG
  ACCTGCGGTATACCTTTATCTATAGCCTTCCCCTAGTTCTTCAGAGCATCAATTTTAACT
  GTGGACATTGAATTTGTGGGACCGCAATCATGACTGTGGCCAAGATANCAGATGCTGCAG
  CCATTGCTATAGAATGAATGATCCTGCAAGAGGCCGAATCCCATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022808 unedited
  CGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAATTGCTCTATGCATTTATT
  ATTAGCTATTATTAATGCAGAATGAGGGAACAAAAAGCTCACAAACACTCTACACAATTT
  CACAAGACGCATTGCAGGGGAAAAAGTGACTGAGATGGATCAACAGTATGCTAAGGTCTT
  GGTGGACGCATTCCTGGGGGAGGTGGACCTCTAATGCCTGGTGGAGGGGGTCTCATTCCC
  GGAGGCGGCATGCCTATTGGCGTCCCTCGAGCAGGGGGAAGCCCAATTGGTGGGCCCATG
  GGTGGTCTCATACCAGGTGGAGGAGCCATAATGCCTGGAGGTGGGGTTGCTCTGCCGACG
  GGTGGGGGCGGAGTGCCCCGTCCTGGTGGGTACTGTGTTGGGGCTCCAGCAATACTGGCA
  GTCGCAGCAACAGCAGCAGCTGCTACAGTGCCTCTTCCCTGTGGAGTCATTACCTGCTGG
  GATGGTCCCCCAACTCCTCGGACAGGGCCTGCCAATCCAGCAGGGGCCTGGGGAATTGGC
  ACACCAGCTGGTACTCCTCTACCAGCTGCCCTACCAACCCCAGGGCCTCCAGCAGCTCCA
  GCAAGTGGTACCCGAGCAATGCCAGTATCTTTGGGGGGTGGCCCCTCCACAGTCATGGAT
  ACCAAGTTCTCCCCACGCAGCATCACCAGACCCAAAACCCGCTTTTCTTCACGCTCTGGT
  TGCTTCGCATTCTTTGGCTTGATCTTTCTGAACTCATCACAATCACAGAGGATCAAATTC
  ATATGCTTGTCAAAGCNCTTAAAGTGCCAATGAAGATTCGGCCATCTTGCAGGATACATC
  TCATTCTATAGNCAATGGGCTGCAGCATCTTGCTACTCTTGCCACAGTCATGGATTGCTG
  TCCACCAATCCATGTCCACAGTAAACTTGAGCTTCTGAAACTAGGGAAGCTTANNATAAG
  CTGACGCAGTTCTCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022808
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022808.2, NP_073719.1
• RefSeq Size: 1643 bp
• RefSeq ORF: 723 bp
• Locus ID: 6638
• Cytogenetics: 15q11.2
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]'
  Transcript Variant: This variant (5) represents use of an alternate upstream promoter compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.