DUSP6 (NM_022652) Human Untagged Clone

CAT#: SC112460

DUSP6 (untagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP6
• Synonyms: HH19; MKP3; PYST1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112460 sequence for NM_022652 edited (data generated by NextGen Sequencing)
  ATGATAGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTG
  GCGTGGCTCAACGAGCAGCTGGAGCTGGGCAACGAGCGGCTGCTGCTGATGGACTGCCGG
  CCGCAGGAGCTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGGCCATCCCGGGC
  ATCATGCTGCGGCGCCTGCAGAAGGGTAACCTGCCGGTGCGCGCGCTCTTCACGCGCGGC
  GAGGACCGGGACCGCTTCACCCGGCGCTGTGGCACCGACACAGTGGTGCTCTACGACGAG
  AGCAGCAGCGACTGGAACGAGAATACGGGCGGCGAGTCGTTGCTCGGGCTGCTGCTCAAG
  AAGCTCAAGGACGAGGGCTGCCGGGCGTTCTACCTGGAAGATGAAGCCCGGGGCAAGAAC
  TGTGGTGTCTTGGTACATTGCTTGGCTGGCATTAGCCGCTCAGTCACTGTGACTGTGGCT
  TACCTTATGCAGAAGCTCAATCTGTCGATGAACGATGCCTATGACATTGTCAAAATGAAA
  AAATCCAACATATCCCCTAACTTCAACTTCATGGGTCAGCTGCTGGACTTCGAGAGGACG
  CTGGGACTCAGCAGCCCATGTGACAACAGGGTTCCAGCACAGCAGCTGTATTTTACCACC
  CCTTCCAACCAGAATGTATACCAGGTGGACTCTCTGCAATCTACGTGA
  
  Clone variation with respect to NM_022652.2
  340 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022652 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGGCTGAGTCC
  AAGAGATAGCAAATCGAGTCTTAAATAATCCGGGGAGAAAGACGCCCGGGTAGATTTGAG
  GTGCAGCCTTGGAGGGAGGGATTAGAAGCCGCTAGACTTTTTTTCCTCCCCTCTCAGTAG
  CACGGAGTCCGAATTAATTGGATTTCATTCACTGGGGAGGAACAAAAACTATCTGGGCAG
  CTTCATTGAGAGAGATTCATTGACACTAAGAGCCAGCGGCTGCAGCTGGGTGCAGAGAGA
  ACCTCCGGCTTTACTTCTGTCTCGTCTGCCCCAACCGCTAGCCTCGGCTTGGGTAAGGCG
  AGGCGGAATTAAACCCCGCTCCGAGAGCGGCAGCTTCGCGCGCGGTGCGCTCGGCCTATG
  CCTGCCCCGAGGGGCGTCTGGTAGGCACCCCGCCCTCTCCCGCAGCTCGACCCCCATGAT
  AGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTGGCGTG
  GCTCAACGAGCAGCTGGAGCTGGGCAACGAGCGGCTGCTGCTGATGGACTGCCGGCCGCA
  GGAGCTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGGCCATCCCGGGCATCAT
  GCTGCGGCGCCTGCAGAAGGGTAAACCTGCCGTGCGCGCGCTCTTCACGCGCGGCGAGGG
  ACCGGACCGCTTCACCCGGCGCTGTGGCACCGACACAGTGGTGCTCTACGACGAGAGCAG
  CAGCGACTGGAACGAGAATACGGNNCGCGAGTCGTTGCTCGGGCTGCTGCTCAAAANCTC
  AAGACGAGGCTGCCGGCCGCTCTACCTGAAGGTGGGCTCAGTAAGTCCAAGCGAGTCTCC
  TGCATGCGAGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022652 unedited
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAGCCAAAATAGTTATT
  TATTAATAATTTAAGGTTTTACATTCTTATAATAAATTCCAGCTCTAAACTTTACACCAC
  GAACATAATGGAGAATTTAACTCTCCCTTCTTCACAATCAAATGCATTTCTCTTATCAAA
  TGGGTACCCATGCTCACACACACACACTTCCAGTTTTATACAAATTTTTTTAAAGGAAAG
  AAACCAACCCAAAGTATTGCATTTGAGGTGACACTCCCTGAAGAATTTTATACAGAGGTA
  ATATACTGTTTAGCACAGCTGAAGGTTTTTTTATTCTTTCTTTTTTTAAAGTGAGCCCAT
  GATTTGGTGTCTTTCCCAGATTATCCCCTTTGCGACAACACAAGCAAAAATATTTACAAA
  GGTAAGGCATAGTCAAACTACATAAAGAGAAAAAATCATGAGGAAAATACATGAAAAAGA
  CTAAAGACTTCGCCATAACAAGGTCTTAGTGATAATAGTGTCCGTAAAGATGTCATCAGA
  ATTGGTAAAGTCAAGCATGCTGCAAATATACCCCTTTGGATTAAAAAAGAGCACAATCTT
  CTTTTTTTGTTNTGTTCTGTCTTAAGAAGTGGCATACTGCTCTTTCTCCCTTTGGATAAT
  TTCTTTTAACCCCATCAAAGGAAAAACAAACCCATTCAAACAACCTGTCAAGTGATCCAA
  GATCAAAATTTACCATAATCAATGGAGGATCCCATTTTTTTTCCAACCGATCCCCAAATA
  CAAACTAAATCTCCTTTTGCCTCCCTCTTTCCAAACTGGACTATCTCTCTTGAAAACATT
  TATTGCCGGTCCAAATCCAAAGGAACCCCTTCAAAGCCCTAACAACAACGGCTCCACCTT
  CATTCGGGCCCACCCTAGCCCTGGGAGCTCCAATCCCACCCCTCCCGGTTACCTCACCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022652
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022652.2, NP_073143.2
• RefSeq Size: 2404 bp
• RefSeq ORF: 708 bp
• Locus ID: 1848
• Cytogenetics: 12q21.33
• Domains: DSPc, RHOD
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting shorter isoform (b) lacks an internal protein segment compared to isoform a.