HLA-DRB3 (NM_022555) Human Untagged Clone

CAT#: SC112463

HLA (untagged)-Human major histocompatibility complex, class II, DR beta 3 (HLA-DRB3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-DRB3
• Synonyms: DRB3; HLA-DPB1; HLA-DR1B; HLA-DR3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112463 sequence for NM_022555 edited (data generated by NextGen Sequencing)
  ATGGTGTGTCTGAAGCTCCCTGGAGGCTCCAGCTTGGCAGCGTTGACAGTGACACTGATG
  GTGCTGAGCTCCCGACTGGCTTTCGCTGGGGACACCCGACCACGTTTCTTGGAGCTGCTT
  AAGTCTGAGTGTCATTTCTTCAATGGGACGGAGCGGGTGCGGTTCCTGGAGAGACACTTC
  CATAACCAGGAGGAGTACNTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGAGG
  GAGCTGGGGCGGCCTGNNGCCGAGTACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAAG
  CGGGGCCAGGTGGACAATTACTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTG
  CAGCGGCGAGTCCATCCTCAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCAC
  CACAACCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGG
  TTCCGGAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACGGGCCTGATCCAGAATGGA
  GACTGGACCTTCCAGACCCTGGTGATGCTAGAAACAGTTCCTCGGAGTGGAGAGGTTTAC
  ACCTGCCAAGTGGAGCACCCAAGCGTAACGAGCCCTCTCACAGTGGAATGGAGTGCACGG
  TCTGAATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTC
  TTCCTTGGGGCCGGGCTGTTCATCTACTTCAGGAATCAGAAAGGACACTCTGGACTTCAG
  CCAACAGGATTCCTGAGCTGA
  
  Clone variation with respect to NM_022555.3
  119 g=>t;164 a=>t;171 c=>g;175 t=>c;197 t=>a;199 c=>n;239 c=>g;257 t=>n;258 c=>n;266 c=>a;308 g=>a;603 t=>c;634 g=>c;654 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022555 unedited
  NNNNGGTTCGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGAGAC
  TTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCTCCAGCATGGTGTGTCTGAAGCTC
  CCTGGAGGCTCCAGCTTGGCAGCGTTGACAGTGACACTGATGGTGCTGAGCTCCCGACTG
  GCTTTCGCTGGGGACACCCGACCACGTTTCTTGGAGCTGCTTAAGTCTGAGTGTCATTTC
  TTCAATGGGACGGAGCGGGTGCGGTTCCTGGAGAGACACTTCCATAACCAGGAGGAGTAC
  GCGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGAGGGAGCTGGGGCGGCCTGAT
  GCCGAGTACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAAGCGGGGCCAGGTGGACAAT
  TACTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTGCAGCGGCGAGTCCATCCT
  CAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCACCACAACCTCCTGGTCTGC
  TCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGGAACGGCCAGGAA
  GAGAAGGCTGGNGGTGGTGTCCACGGGCCTGATCCAGAATGGAGACTGGACCTTCCAGAC
  CCTGGTGATGCTAGAAACAGTTCCTCGGAGGGGNAGAAGGTTTTACACCTGCCAAGTGGA
  GCACCCAAGCGTAACGAGCCCTCTCAAGTGGAATGGAGTGCACGGGTCTGATCTGCACAG
  AGCAGATGCTGAGTGGAGTTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCTGGNGCCCGGG
  CTGNTCATCTACTTCAGGAATCAGAAGACACTCTGGACTTAGCCACAGGATTCTGANCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022555 unedited
  GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATATTTAA
  CTCCATCTTTGAGAAAATTTAATAATGTAATGTGTTTGTGGTACAGGGTGAGTACAGATG
  CACAGGAGGCCATAGGGTTTAGGCAAAGGGGAGCACAAAAGTTGAAGATGAGGCGCTGCC
  ATCAATGCTGGGACTTCAGGCCAAGGGCAGGAACTGAGGAAGCCACAAGGGAGGACATTT
  TCTGCAGTTGCTGAACCAGTAGCAACTAGGTCCTGAGAAAGCCCTCTCTCGTGGAAGAAT
  AACAGCCAGGCGGGAAAGCTTTTCATCCTGCAAAGCTGGGGAAGAAGATTCTTCCTTAAA
  TTGTCATCTGCACTTCAGCTCAAGAATCCTGTTGGCTGAAGTCCAGAGTGTCCTTTCTGA
  TTCCTGAAGTAGATGAACAGCCCGGCCCCAAGGAAGAGCAGGCCCAGCACAAAGCCCCCG
  ACTCCACTCAGCATCTTGCTCTGTGCAGATTCAGACCGTGCACTCCATTCCACTGTGAGA
  GGGCTCGTTACGCTTGGGTGCTCCACTTGGCAGGTGTAAACCTCTCCACTCCGAGGAACT
  GTTTCTAGCATCACCAGGGTCTGGAAGGTCCAGTCTCCATTCTGGATCAGGCCCGTGGAC
  ACCACCCCAGCCTTCTCTTCCTGGCCGTTCCGGAACCACCTGACTTCAATGCTGCCTGGA
  TAGAAACCACTCACAGAGCAAACCANGAAGTTGTGGTGCTGCAGGGGCTGGGTCTTTGCA
  AGATACACAGTCACCCTGAGATGGACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCG
  TAGNTGTGTCTGCAGTAATTTGTCCACTGGNCCCGCTTCTGCTCCAGNAGGTCCTTCGGG
  CTGTTCAGTACTCGGNATTAAGCCGCCCCANTTCCTCACCGGCNGGTACTTCCCCACTGG
  CTTTTGAAACCCCGTTCTTCTCCTGTTTTGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022555
• Insert Size: 1240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022555.3, NP_072049.2
• RefSeq Size: 1158 bp
• RefSeq ORF: 801 bp
• Locus ID: 3125
• Cytogenetics: 6p21.3
• Domains: MHC_II_beta, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: 'HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene. [provided by RefSeq, Feb 2020]'