DGCR8 (NM_022720) Human Untagged Clone

CAT#: SC112466

DGCR8 (untagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGCR8
• Synonyms: C22orf12; DGCRK6; Gy1; pasha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112466 sequence for NM_022720 edited (data generated by NextGen Sequencing)
  ATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGATG
  GAGAGCCGAGCTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCACCACCTCCCCTG
  CAAACGTCCAGTGGTGCAGAGGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAA
  CTCCCTGCTGAGGACCCCTTCAACTTCTACGGAGCTTCTCTTCTCTCCAAAGGATCCTTC
  TCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCCACAGCCCGCGCACCGCCCGG
  CACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGATGTAAAGATT
  AGCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGCA
  GAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGCCCTGTCAGTGGGGACGTGCAT
  GCTTGTCCCTTTGGCGGGAGTGTTGGTGACGGGGTAGGCATAGGGGGTGAGAGTGCTGAT
  AAGAAGGATGAGGAGAATGAGCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGAT
  GAGTTAGAAGATTTTACTGACAATTTGGAGCTAGATGAAGAAGGAGCAGGCGGGTTCACG
  GCTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGGCCTTGAATTTCCCCTACGAG
  GATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAAGGCCTTTGTGCCCCCAAAAAG
  AGGCGAACAGAGGAAAAATATGGCGGAGACAGCGACCATCCGTCCGATGGAGAGACAAGT
  GTGCAGCCGATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACA
  GAGCCGCTGCCCGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCTA
  CACAGAGAGTCTCGGGTGGTCACCTGGTCCAGGCCATACTTCTTGGGAACGGGAAGCATA
  CGGAAACACGACCCTCCTCTGAGTAGCATCCCTTGTCTGCATTATAAGAAAATGAAGGAC
  AACGAGGAACGGGAGCAAAGCAGTGACCTCACCCCTAGTGGGGATGTGTCCCCCGTCAAG
  CCCCTGAGCCGATCTGCAGAGCTGGAGTTTCCCCTGGATGAGCCTGACTCTATGGGTGCT
  GACCCGGGGCCCCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCTG
  GGGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATCTCGAGGAATTT
  CGAAGCTACCTGGAGAAGCGTTTTGACTTTGAGCAAGTTACTGTGAAAAAATTCAGGACT
  TGGGCTGAGCGGCGGCAATTCAATCGGGAAATGAAGCGGAAGCAGGCGGAGTCCGAGAGG
  CCCATCTTGCCAGCCAATCAGAAGCTCATTACTTTATCAGTGCAAGATGCACCCACAAAG
  AAAGAGTTTGTTATTAACCCCAACGGGAAATCCGAGGTCTGCATCCTGCACGAGTACATG
  CAGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGAGAACCCAAGTGAG
  CCTTTTGGTGCCTCGGTGACCATTGATGGTGTGACTTACGGATCTGGAACTGCAAGCAGC
  AAAAAACTTGCGAAGAATAAAGCTGCCCGAGCTACACTGGAAATCCTCATCCCTGACTTT
  GTTAAACAGACCTCTGAAGAGAAGCCCAAAGACAGTGAAGAACTCGAGTATTTTAACCAC
  ATCAGCATCGAGGACTCGCGGGTCTACGAGCTGACCAGCAAGGCTGGGCTGTTGTCTCCA
  TATCAGATCCTCCACGAGTGCCTTAAAAGAAACCATGGGATGGGTGACACGTCTATCAAG
  TTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATACGTCATGGCGTGTGGCAAGCAC
  ACAGTGCGCGGGTGGTGTAAGAACAAGAGAGTTGGAAAGCAGTTAGCCTCACAGAAGATC
  CTTCAGCTGCTGCACCCACATGTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGT
  GAGAGCAGCAAGATGGTCAAGCAGGAGACATCGGACAAGAGTGTGATTGAGCTGCAGCAG
  TATGCCAAGAAGAACAAGCCCAACCTGCACATCCTCAGCAAGCTCCAAGAGGAGATGAAG
  AGGCTAGCTGAGGAAAGGGAGGAGACTCGAAAGAAGCCCAAGATGTCCATTGTGGCGTCC
  GCCCAGCCTGGCGGTGAGCCCCTGTGCACCGTGGACGTGTGA
  
  Clone variation with respect to NM_022720.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022720 unedited
  GGGTCANAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCTGTGG
  TTTGGCTGCGGGCGGCTTGGGCAGCCCGCGGGCGCCTCAGGTAGAAGAAGAAAGGTGCCA
  CTCCGGCATGAAGACAGACTCGCTTAGTCGCCAGTCACTTAAGCTGAGTGCATTGTGATT
  TCCAATAATTGAGGCAGTGGTTCTAAAAGCTGTCTACATTAATGAAAAGAGCAATGTGGC
  CAGCTTGACTAAGCCGCCAGCGCACAGCGCGGCAGGACGCGCCCGGGTATCAGCGGAAAT
  GTGCATGTTAGCTGTGTAGATTTATGTGAGGGCTTGTAAAACTCTGGTCTTGTAAACTAG
  TCTTAAGCGCTTTTAATATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCG
  CAGGAGAAGCGGTGATGGAGAGCCGAGCTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGT
  CTCCACCACCTCCCCTGCAAACGTCCAGTGGTGCAGAGGTAATGGACGTTGGCTCTGGTG
  GTGATGGACAGTCCGAACTCCCTTGCTGAGGACCCCTTCAACTTCTACGGAGCTTCTCTT
  CTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCCAC
  AGCCCGCGCACCGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTG
  CTTAAGGATGTAAAGATTAGCGTGAGCTTTACCGAGAGCTGCANGAGTAAGGACAGGAAG
  GTGCTGTACACAGGAGCANAGCGCGACNTGCGGGCGGAGTGCGGTCTGCTCCTTACCCTG
  TCAGTGGGGACGTGCATGCTTGTCCCTTTGCGGNNANNTGTGTGACNGNGTANGCATAGG
  GGGTGAGAGTGCTGATANAAAGATGNNAGAAATGAGCTGGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022720 unedited
  AGTTTGCTTGTTGTGTTTAGGACATCAAGGCTCGCCTTCCAGCACAGCCCAGTGCCCTGG
  CTGGCATCAGCACAGGCTCCCCTGCCCCGGCCTTGAAGCATGGCTGTGTGCACGATTCAT
  ACCCATGCTTGGCAGACGGGTGGGGGGGGGGGGGGCCCACAAAAACCACAAAAGTATGGC
  AGCCCTTGCTGTGGGGGAGCCTGGGAACAACAAAACACAGCGAGGCTCCCCAACCTTTAG
  GGGAACACATGTCCACAAAGCTTTGTTTCACAGGCGCATCTGGCTGGTTAAGGGCAACAG
  TCACAGTCAAATGACTTTTCACAGCTGCAAACAGGAGGCAAAACAAAAGCAAGTGTTTAA
  TTATAGCAATTTGAGTTGAGGGTTTTTTTTTTAAAGGTCAACAAAAGCCAACCTTGTTCA
  CACAGGTAGTGAGGGAAGGATATGTTGTGGCCGGCCCACAGGCCAACATTGTTTTCCTGA
  CAAAAAAAAAAAAAAAAAAGCGCCATCATTCCCGCCTGTAGGGGGCATGGTGGGGGACAA
  ACACGGCAAAACGCTGGACTCTTGCTTAAAATGGCGCACCACAAGCACACGGAACTCTGC
  ACAGGTGCCATTCCACCACCAGGGCCAGCCTTTGAGCACACCCTGGTGCCTAAGACTCCG
  GCAGGAACACATTTCCATCGCACACCCACCCTTAGGGGCCACCAGTCCAAGAAACAAGCT
  GGCCACCAGGCAGTTGCCTTGAAATCCAGAAAAACCGGTGTCACAGGCAACAGTTTTGGA
  TAAGAAATGGCCTACATTGAAGGCTTTTGCTCCTTGTTTACAAGCCCTCTGGCTTAAAGG
  CTGATTCCAAATTACCTCCTATTTCATTGGGAAGTCTTCAAAAGGCTAAAATCACAAAAC
  TTAAAATTCAATGGGGGACATATGACCCCCCGAAAAAATCTTGNAAAAACTTGCCCGAGG
  CAAAAAAGCAAAGGGCCTCAGGTCCAAAGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022720
• ORF Size: 2322 bp
• Insert Size: 4350
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_022720.5, NP_073557.3
• RefSeq Size: 4461
• RefSeq ORF: 2322
• Locus ID: 54487
• Gene Summary: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.