SMURF 2 (SMURF2) (NM_022739) Human Untagged Clone

CAT#: SC112475

SMURF2 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMURF2
• Synonyms: DKFZp686F0270; MGC138150
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022739 edited
  GAATTCGGCACGAGGGGAAGCGGCCGAGCCCAGAGTTCTGCTCCGGCGCCCCCGAGCACC
  GCCCGCTTCAGCCGACCAGCCCCGTCGGCTACTGGGCCTCGCCGAGACGAGAGGAGGGAA
  AGGCCTCGGCGGCCGCCGCCCCCGCCGCCGGGGACATGTCTAACCCCGGAGGCCGGAGGA
  ACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACCTGGTGAAAAAGGATT
  TTTTCCGACTTCCTGATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAATGCCATT
  CTACAGATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGACCTGTATA
  TTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGAAGATCCATAAGAAAC
  AAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGCCTCAAAG
  ACACTGGTTATCAGAGGTTGGATTTATGCAAACTCGGGCCAAATGACAATGATACAGTTA
  GAGGACAGATAGTAGTAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGACAAGTTG
  TGGACTGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGGAGAACCG
  CCTCTGGAAGAATCCAGTATCTAAACCATATAACAAGAACTACGCAATGGGAGCGCCCAA
  CACGACCGGCATCCGAATATTCTAGCCCTGGCAGACCTCTTAGCTGCTTTGTTGATGAGA
  ACACTCCAATTAGTGGAACAAATGGTGCAACATGTGGACAGTCTTCAGATCCCAGGCTGG
  CAGAGAGGAGAGTCAGGTCACAACGACATAGAAATTACATGAGCAGAACACATTTACATA
  CTCCTCCAGACCTACCAGAAGGCTATGAACAGAGGACAACGCAACAAGGCCAGGTGTATT
  TCTTACATACACAGACTGGTGTGAGCACATGGCATGATCCAAGAGTGCCCAGGGATCTTA
  GCAACATCAATTGTGAAGAGCTTGGTCCATTGCCTCCTGGATGGGAGATCCGTAATACGG
  CAACAGGCAGAGTTTATTTCGTTGACCATAACAACAGAACAACACAATTTACAGATCCTC
  GGCTGTCTGCTAACTTGCATTTAGTTTTAAATCGGCAGAACCAATTGAAAGACCAACAGC
  AACAGCAAGTGGTATCGTTATGTCCTGATGACACAGAATGCCTGACAGTCCCAAGGTACA
  AGCGAGACCTGGTTCAGAAACTAAAAATTTTGCGGCAAGAACTTTCCCAACAACAGCCTC
  AGGCAGGTCATTGCCGCATTGAGGTTTCCAGGGAAGAGATTTTTGAGGAATCATATCGAC
  AGGTCATGAAAATGAGACCAAAAGATCTCTGGAAGCGATTAATGATAAAATTTCGTGGAG
  AAGAAGGCCTTGACTATGGAGGCGTTGCCAGGGAATGGTTGTATCTCTTGTCACATGAAA
  TGTTGAATCCATACTATGGCCTCTTCCAGTATTCAAGAGATGATATTTATACATTGCAGA
  TCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCCACTTTGTTGGACGAA
  TAATGGGAATGGCTGTGTTTCATGGACATTATATTGATGGTGGTTTCACATTGCCTTTTT
  ATAAGCAATTGCTTGGGAAGTCAATTACCTTGGATGACATGGAGTTAGTAGATCCGGATC
  TTCACAACAGTTTAGTGTGGATACTTGAGAATGATATTACAGGTGTTTTGGACCATACCT
  TCTGTGTTGAACATAATGCATATGGTGAAATTATTCAGCATGAACTTAAACCAAATGGCA
  AAAGTATCCCTGTTAATGAAGAAAATAAAAAAGAATATGTCAGGCTCTATGTGAACTGGA
  GATTTTTACGAGGCATTGAGGCTCAATTCTTGGCTCTGCAGAAAGGATTTAATGAAGTAA
  TTCCACAACATCTGCTGAAGACATTTGATGAGAAGGAGTTAGAGCTCATTATTTGTGGAC
  TTGGAAAGATAGATGTTAATGACTGGAAGGTAAACACCCGGTTAAAACACTGTACACCAG
  ACAGCAACATTGTCAAATGGTTCTGGAAAGCTGTGGAGTTTTTTGATGAAGAGCGACGAG
  CAAGATTGCTTCAGTTTGTGACAGGATCCTCTCGAGTGCCTCTGCAGGGCTTCAAAGCAT
  TGCAAGGTGCTGCAGGCCCGAGACTCTTTACCATACACCAGATTGATGCCTGCACTAACA
  ACCTGCCGAAAGCCCACACTTGCTTCAATCGAATAGACATTCCACCCTATGAAAGCTATG
  AAAAGCTATATGAAAAGCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAAT
  GACAAGCTTCAAGGATTTACCCAGGACTCTATTTATACAACCCTGACTGACAGCCTCCTT
  TCAGCAGAGTTTCAAAGAATATGCTGAAATACAGGAAAACACTCCCCCCCCCCCTTTTTT
  TTTTTTTTTTTTACATTTTAGGACACTGTGAGGGGAAAGGACAATTTTGAAATTCCTTTT
  CAAGGAAAAAAAAGGTCTTTATGCTTTGCCATGAGGCCACATTCAGCTGCTATTTAAACT
  TAATATCTTGAACCTAAAGAATGCTGACTTTTCCTACATTTCCAGAGTTAGGCAGTATTC
  TACACTTAAAGACTACTACTATTTTTATAAAAGGTAATCTATTCAAATTTCTTCACAGAT
  TTCAAGTCTCTCAAACATCAAGACAACTTCAGCAGTCGGTACAAGTCACATTTCATTTTG
  ATTGAATACATGATCTTGAACAGCTCCTGTACTTGCTCTTTGTAAAAAAAAATAAAATTA
  TTTTGAATTATTCTACCTTTGTAAACAATTGGCTAAAAGAATCATCTTTAAGAAATTAAG
  CCATTTACATGTTTGTGTTTTTCTATAGCAGAGCATTATATTTTGCATTATATGTTTCAA
  CCTAGTCTAAGTGGGTCTTTTTTACATTTTTCAAGAACGGATTTCCTGGAATACAGCGAT
  ATAATTTTGGTTGTCAAATTCCTAATGCAACCATTTAGTCTAAACTTAGTCATTTATTTG
  TGACAATAAGATGTGTTCAGGGGCTCCCTGTTTTTAAGAGACTCTTTTAAAAAAAAAAAA
  ACCTAATGTTTTTATCTTGAGTCAATATGATTAGGTATTTTGGATTTACTTTTAATCTTA
  AAATACTGCATTTTTATAGCTTCTCAGAGCATGTGGATGGGATGGGATTTTCGTTATTTT
  GCTGGGTCAGCTTATCTXXXXXXXXXXXXXXXXXXXXXXXXXGAGATTGCAGTGAGCCGA
  GATCGCACCACTGCACTCCAGCCTGGGCGACAAGAGTGAGACTCCGTCCCCGCCCAAAAA
  AAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022739 unedited
  NGGCTTCTTACCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
  CGGCCGCGAATTCGGCACGAGGGGAAGCGGCCGAGCCCAGAGTTCTGCTCCGGCGCCCCC
  GAGCACCGCCCGCTTCAGCCGACCAGCCCCGTCGGCTACTGGGCCTCGCCGAGACGAGAG
  GAGGGAAAGGCCTCGGCGGCCGCCGCCCCCGCCGCCGGGGACATGTCTAACCCCGGAGGC
  CGGAGGAACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACCTGGTGAAA
  AAGGATTTTTTCCGACTTCCTGATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAA
  TGCCATTCTACAGATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGAC
  CTGTATATTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGAAGATCCAT
  AAGAAACAAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGC
  CTCAAAGACACTGGTTATCAGAGGTTGGATTTATGCAAACTCGGGCCAAATGACAATGAT
  ACAGTTAGAGGACAGATAGTAGTAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGA
  CAAGTTGTGGACTGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGG
  AGAACCGCCTCTGGAAGATCCAGTNTCTAAACCATATAAACAGAACTANCGCATGGGAGC
  GCCNAACACGACCGGCATCCCGATATTCTAGCCCTGGCAGACCTCTTAGCTGCTTTGNTG
  ATGAGAACACTCCCATTAGTGGAACAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022739
• ORF Size: 2247 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022739.3, NP_073576.1
• RefSeq Size: 3866
• RefSeq ORF: 2247
• Locus ID: 64750
• Domains: C2, HECT, WW
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, TGF-beta signaling pathway, Type I diabetes mellitus, Ubiquitin mediated proteolysis, Viral myocarditis
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProtKB/Swiss-Prot Function]