CDC25B (NM_021872) Human Untagged Clone

CAT#: SC112556

CDC25B (untagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC25B
• Synonyms: cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021872 edited
  GAATTCGGCACGAGGCTCTCCTCCCTCGCGCCTGGCCCTGTGGCTCTTCCTCCCTCCCTC
  CTTCCCCCCCCCCCCACCCCTCGCCCGCTGCCTCCCTCGGCCCAGCCAGCTGTGCCGGCG
  TTTGTTGGCTGCCCTGCGCCCGGCCCTCCAGCCAGCCTTCTGCCGGCCCCGCCGCGATGG
  AGGTGCCCCAGCCGGAGCCCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTG
  GCGCCCAGCGTCCGGGCCACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGT
  CCCCGGTGCGGGCGGCCGCTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACC
  TCGCCGGGCTCGGCAGCGAAACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCC
  GCAGCCGCCTGACGCACCTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTG
  AATCCTCCGAATCTTCTGATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCC
  ACATGGCGGAGCAGACGTTTGAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACG
  AGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTGAGGCTGCTGGGCCACAGCCCCG
  TGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAGGCGGGCA
  GTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGC
  CATGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGG
  AAGCCTTTGCCCAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGA
  AGATGGAAGTGGAGGAGCTCAGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAG
  AGGGGGATACTGAGGAAGATGATGGATTTGTGGACATCCTAGAGAGTGACTTAAAGGATG
  ATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGTGCCCCACTGGTCAAGACCTTGG
  AAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGGCTCTTCCGCTCTC
  CGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAGGACA
  GGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGG
  AGGCTGAGGAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCG
  AGAACCTCCTGGACAGTGACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCC
  TACAGACAGTAGACGGAAAGCACCAAGACCTCAAGTACATCTCACCAGAAACGATGGTGG
  CCCTATTGACGGGCAAGTTCAGCAACATCGTGGATAAGTTTGTGATTGTAGACTGCAGAT
  ACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTGAACTTGCCCCTGGAACGCG
  ACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGACAAGAGAGTCA
  TCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATCA
  GGGAACGAGACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCC
  TGAAAGGCGGCTACAAGGAGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACT
  ACCGGCCCATGAACCACGAGGCCTTCAAGGATGAGCTAAAGACCTTCCGCCTCAAGACTC
  GCAGCTGGGCTGGGGAGCGGAGCCGGCGGGAGCTCTGTAGCCGGCTGCAGGACCAGTGAG
  GGGCCTGCGCCAGTCCTGCTACCTCCCTTGCCTTTCGAGGCCTGAAGCCAGCTGCCCTAT
  GGGCCTGCCGGGCTGAGGGCCTGCTGGAGGCCTCAGGTGCTGTCCATGGGAAAGATGGTG
  TGGGTGTCCTGCCTGTCTGCCCCAGCCCAGATTCCCCTGTGTCATCCCATCATTTTCCAT
  ATCCTGGTGCCCCCCACCCCTGGAAGAGCCCAGTCTGTTGAGTTAGTTAAGTTGGGTTAA
  TACCAGCTTAAAGGCAGTATTTTGTGTCCTCCAGGAGCTTCTTGTTTCCTTGTTAGGGTT
  AACCCTTCATCTTCCTGTGTCCTGAAACGCTCCTTTGTGTGTGTGTCAGCTGAGGCTGGG
  GGAGAGCCGTGGTCCCTGAGGATGGGTCAGAGCTAAACTCCTTCCTGGCCTGAGAGTCAG
  CTCTCTGCCCTGTGTACTTCCCGGGCCAGGGCTGCCCCTAATCTCTGTAGGAACCGTGGT
  ATGTCTGCCATGTTGCCCCTTTCTCTTTTCCCCTTTCCTGTCCCACCATACGAGCACCTC
  CAGCCTGAACAGAAGCTCTTACTCTTTCCTATTTCAGTGTTACCTGTGTGCTTGGTCTGT
  TTGACTTTACGCCCATCTCAGGACACTTCCGTAGACTGTTTAGGTTCCCCTGTCAAATAT
  CAGTTACCCACTCGGTCCCAGTTTTGTTGCCCCAGAAAGGGATGTTATTATCCTTGGGGG
  CTCCCAGGGCAAGGGTTAAGGCCTGAATCATGAGCCTGCTGGAAGCCCAGCCCCTACTGC
  TGTGAACCCTGGGGCCTGACTGCTCAGAACTTGCTGCTGTCTTGTTGCGGATGGATGGAA
  GGTTGGATGGATGGGTGGATGGCCGTGGATGGCCGTGGATGCGCAGTGCCTTGCATACCC
  AAACCAGGTGGGAGCGTTTTGTTGAGCATGACAGCCTGCAGCAGGAATATATGTGTGCCT
  ATTTGTGTGGACAAAAATATTTACACTTAGGGTTTGGAGCTATTCAAGAGGAAATGTCAC
  AGAAGCAGCTAAACCAAGGACTGAGCACCCTCTGGATTCTGAATCTCAAGATGGGGGCAG
  GGCTGTGCTTGAAGGCCCTGCTGAGTCATCTGTTAGGGCCTTGGTTCAATAAAGCACTGA
  GCAAGTTGAGAAACCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021872 unedited
  GGTTCAAATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCTC
  CTCCCTCGCGCCTGGCCCTGTGGCTCTTCCTCCCTCCCTCCTTCCCCCCCCCCCCACCCC
  TCGCCCGCTGCCTCCCTCGGCCCAGCCAGCTGTGCCGGCGTTTGTTGGCTGCCCTGCGCC
  CGGCCCTCCAGCCAGCCTTCTGCCGGCCCCGCCGCGATGGAGGTGCCCCAGCCGGAGCCC
  GCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCCAGCGTCCGGGCCAC
  CTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGCCGCT
  TCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCGAA
  ACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCCGCAGCCGCCTGACGCACCTA
  TCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTGAT
  GCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTT
  GAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGC
  TTCCAGTCTATGCCGGTGAGGCTGCTGTGCCACAGCCCCGTGCTTCGGACATCACCAACT
  CCCAGCGCCCGACGGCCGGAGGAGAGCGAAGCGGGCAGTGTAGCTGCCAACAGCTCTGGG
  GGAAGACAAGAAGATGATGGATTTGGTCTTCAAGAGCCATGGNAGNCCACACATTCCACT
  CCACCCATGCTCTGCCAAAGTGGCCACCCGCAGGGAGCCTTTGCCCAGAGACCAGCTCGC
  CCCCGACCTGATGTGTCTAGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021872 unedited
  CGCTTTTAGTATCGAGTTTTTTTTTTTTTTTTTTGGTTCTCTCAACTTGTCTCAGTGGCT
  TTATTTGAACCAAGGGCCCTAACAGATGACTCAGCAGGGCCTTCAAGCACAGCCCTGCCC
  CCATCTTGAGATTCAGAATCCAGAGGGTGCTCAGTCCTTGGTTTAACTGCTTCTGTGACA
  TTTCCTCTTGAATAGCTCCAAACCCTAAGTGTAAATATTTTTGTCCACACAAATAGGCAC
  ACATATATTCCTGCTGCAGGCTGTCATGCTCAACAAAACGCTCCCACCTGGTTTGGGTAT
  GCAAGGCACTGCGCATCCACGGGCATCCACGGGCATCCACCCATCCATCCAACCTTCCAT
  CCATCCGCAACAAGACAGCAGCAAGTTCTGAGCAGTCAGGCCCCAGGGTTCACAGCAGTA
  GGGGCTGGGCTTCCAGCAAGCTCATGATTCAAGCCTTAACCCTTGCCCTGGGAGCCCCCA
  AGGATAATAACATCCCTTTCTGGGGCAACAAAACTGGGACCGAGTGGGTAACTGATATTT
  GACAGGGGAACCTAAACAGTCTACGGAAGTGTCCTGAGATGGGCGTAAAGTCAAACAGAC
  CAAGCACACAGGTAACACTGAAATANGAAAGAGTAAGAGCTTCTGTTCAAGCTGGAGGTG
  CTCGTATGGTGGGACAGGAAAGGGGAATAGAGAAGGGGGCACATGGCAGACATACACCGT
  TCCTACAAAGATAGGGGCAGCCCTGGCCCGGAAGTCACAGGGCAGGAGCTGACCTCAGCC
  AGAATGAGTTAGCTCTGACCATCCTAAGGACACCGGTTTCCCCAGCTCACTGAACAACAC
  AAGGACGTTTAGACCAGAAAATAAGGTTAACCTACAGGAACAGAGCTCTGAGAACAATCC
  GCCTTAACTGGTTACCCACTATCACTAAAAACTGCTTTCAGGGTGGGGCCCGTATTGAAT
  GTGGTGACAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021872
• Insert Size: 3120 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021872.2, NP_068658.1
• RefSeq Size: 3578 bp
• RefSeq ORF: 1620 bp
• Locus ID: 994
• Cytogenetics: 20p13
• Domains: RHOD
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation
• Gene Summary: 'CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3), also known as CDC25B2, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3) compared to isoform 1.