MPST (NM_021126) Human Untagged Clone

CAT#: SC112573

MPST (untagged)-Human mercaptopyruvate sulfurtransferase (MPST), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MPST
• Synonyms: MST; TST2; TUM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021126, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGCCAGGAAGCCGGGAGTCCGAGACCCGGGCCCGCAGCCCGAGTGTCGCCGCCATGGCTTCGC
  CGCAGCTCTGCCGCGCGCTGGTGTCGGCGCAATGGGTGGCGGAGGCGCTGCGGGCCCCGCGCGCTGGGCA
  GCCTCTGCAGCTGCTGGACGCCTCCTGGTACCTGCCGAAGCTGGGGCGCGACGCGCGACGCGAGTTCGAG
  GAGCGCCACATCCCGGGCGCCGCTTTCTTCGACATCGACCAGTGCAGCGACCGCACCTCGCCCTACGACC
  ACATGCTGCCCGGGGCCGAGCATTTCGCGGAGTACGCAGGCCGCCTGGGCGTGGGCGCGGCCACCCACGT
  CGTGATCTACGACGCCAGCGACCAGGGCCTCTACTCCGCCCCGCGCGTCTGGTGGATGTTCCGCGCCTTC
  GGCCACCACGCCGTGTCACTGCTTGATGGCGGCCTCCGCCACTGGCTGCGCCAGAACCTCCCGCTCAGCT
  CCGGCAAGAGCCAACCTGCTCCCGCCGAGTTCCGCGCTCAGCTCGACCCCGCCTTCATCAAGACCTACGA
  GGACATCAAGGAGAACCTGGAATCCCGGCGCTTCCAGGTGGTGGACTCCCGAGCCACTGGCAGGTTCCGC
  GGCACCGAGCCCGAGCCCCGAGACGGCATTGAACCTGGCCACATCCCAGGTACCGTGAACATCCCCTTCA
  CAGACTTCCTGAGCCAGGAGGGGCTGGAGAAGAGCCCTGAGGAGATCCGCCATCTGTTCCAGGAGAAGAA
  AGTGGACCTGTCTAAGCCACTGGTGGCCACGTGTGGCTCTGGCGTCACAGCCTGCCACGTGGCACTAGGG
  GCCTACCTCTGCGGCAAGCCAGACGTGCCCATCTACGATGGCTCCTGGGTGGAGTGGTACATGCGCGCCC
  GGCCCGAGGATGTCATCTCAGAGGGCCGGGGGAAGACCCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021126 unedited
  TCACCATTTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGTGCAGGCCCGC
  AGCCCGAGTGTCGCCGCCATGGCTTCGCCGCAGCTCTGCCGCGCGCTGGTGTCGGCGCAA
  TGGGTGGCGGAGGCGCTGCGGGCCCCGCGCGCTGGGCAGCCTCTGCAGCTGCTGGACGCC
  TCCTGGTACCTGCCGAAGCTGGGGCGCGACGCGCGACGCGAGTTCGAGGAGCGCCACATC
  CCGGGCGCCGCTTTCTTCGACATCGACCAGTGCAGCGACCGCACCTCGCCCTACGACCAC
  ATGCTGCCCGGGGCCGAGCATTTCGCGGAGTACGCAGGCCGCCTGGGCGTGGGCGCGGCC
  ACCCACGTCGTGATCTACGACGCCAGCGACCAGGGCCTCTACTCCGCCCCGCGCGTCTGG
  TGGATGTTCCGCGCCTTCGGCCACCACGCCGTGTCACTGCTTGATGGCGGCCTCCGCCAC
  TGGCTGCGCCAGAACCTCCCGCTCAGCTCCGGCAAGAGCCAACCTGCTCCCGCCGAGTTC
  CGCGCTCAGCTCGACCCCGCCTTCATCAAGACCTACGAGGACATCAAGGAGAACCTGGAA
  TCCCGGCGCTTCCAGGTGGTGGACTCCCGAGCCACTGGCAGGTTCCGCGGCACCGAGCCC
  GAGCCCCGAGACGGCATTGAACCTGGCCACATCCCAGGTACCGTGAACATCCNCTTCACA
  GACTTCCTGAGCCAGAAGGGGCTGGAGAAGAGCCCTGAGGAGATCCGNCATCTGTTCCCA
  GAGAAGAAAGTGGNACCTGTCTAGCCACTGGTGGCCACGTGTGGCTCTGGCGTCACAGCC
  TGCCACGTGGCACTAGGGGCCTACCTCTGCGGCAGCCAGACGTCCCATCTACGATGCTNC
  TGGGTGGAGTGTACATGCGCGCCCGGCCGAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021126 unedited
  GGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAGCACTTGGTTATTTTATTTCC
  TCAAAAATAAAACAGAAAGGAGGCGGGGCCCCAGCTCAGCACCAGGTGGGCAGCTCGGAG
  TTGTGGCCTCCCTCCTCCAGGGCTCGCCTCCGCCTTTCCTCCCGCCCCTACTGGCAGCCA
  ACAAGTCAACGGAATTCCTGGCCTTTGAGATGTCACCCCAAATGCCACCTGAGTTGAGTG
  AAGAAACACTCTCTTGGTGAAAGCAGAAGCGGAGCTACCAGGCCAGGCATTGCTGGTGGC
  CGGCATCACCTGAGCTCGCCTGTGTCCTGCCCAGCTTCAGTGGGTCTTCCCCCGGCCCTC
  TGAGATGACATCCTCGGGCCGGGCGCGCATGTACCACTCCACCCAGGAGCCATCGTAGAT
  GGGCACGTCTGGCTTGCCGCAAAGGTAGGCCCCTATTGCCACGTGGCAGGCTGTGACGCC
  AGAGCCACACGTGGCCACCAGAGGCTTAGACAGGTCCACTTTCTTCTCCTGGAACAGATG
  GCGGATCTCCTCAAGGCTCTTCTCCAACCACTCCTGGCTCAAGAAGTCTGTGAAGGGGAT
  GTTCACGGTACCTGAGATGTGGCCAGGTTCAATGCCGTCTCGGAGCTCGGGCTCGGTGCC
  CGCGGACCCTGCCATGGCTCGGGAGTCCACCCCCTGGAAGCCCCGGGATCCCAGGTCCCC
  CTGATGACCCCTAAGCCTTGATAAAGCCGGGTCCAACCGACCCCGAACCTGGCGGAACCA
  AGTTGCCTTTGCCGGACTGACCGGAAGTTCTGCCCAACCCGGCGGAGCCCACTCATCCCT
  ACCCCGCTGGGGCCCAGGCCCGAACCATCCAAACCCCGGGCGGAACAAAGCCTGACCCGC
  GCATAAACACAAGGGGGGCCCCCCCCCCATCGACTCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021126
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021126.4, NP_066949.2
• RefSeq Size: 1450 bp
• RefSeq ORF: 954 bp
• Locus ID: 4357
• Cytogenetics: 22q12.3
• Domains: RHOD
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).