Dynamitin (DCTN2) (NM_006400) Human Untagged Clone

CAT#: SC112693

DCTN2 (untagged)-Human dynactin 2 (p50) (DCTN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCTN2
• Synonyms: DCTN50; DYNAMITIN; HEL-S-77; RBP50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112693 sequence for NM_006400 edited (data generated by NextGen Sequencing)
  ATGGCGGACCCTAAATACGCCGACCTTCCCGGCATTGCCAGGAATGAGCCAGATGTTTAT
  GAAACTAGCGACCTACCTGAGGATGATCAAGCGGAGTTCGATGCGTTTGCACAAGAGCTG
  GAGGAGCTGACAAGCACAAGTGTGGAACACATCATTGTCAATCCTAATGCTGCCTATGAC
  AAGTTCAAGGACAAGAGAGTGGGGACAAAGGGACTTGATTTCTCAGATCGTATTGGAAAA
  ACCAAGAGGACAGGATATGAATCTGGAGAATATGAGATGCTTGGAGAGGGTCTGGGAGTG
  AAGGAGACACCCCAGCAAAAGTACCAGCGCCTACTGCATGAGGTCCAAGAGCTGACAACT
  GAAGTTGAAAAAATCAAGACGACAGTGAAGGAGTCAGCCACAGAGGAGAAGCTGACCCCT
  GTGTTGCTGGCTAAACAGCTGGCAGCCCTGAAGCAGCAGCTGGTTGCTTCCCACCTGGAG
  AAGCTGCTGGGACCAGATGCTGCAATCAACCTTACCGACCCCGATGGCGCCCTGGCTAAG
  CGCCTACTACTGCAGCTGGAAGCAACAAAGAACAGCAAAGGGGGATCAGGGGGAAAAACC
  ACTGGGACCCCCCCAGATAGCAGCCTTGTCACTTATGAACTACATTCTCGGCCTGAGCAG
  GACAAGTTCTCTCAAGCTGCCAAAGTCGCAGAACTTGAAAAGCGCCTGACAGAGCTGGAG
  ACAGCTGTACGTTGTGATCAGGATGCTCAGAATCCCCTTTCTGCAGGTCTACAGGGAGCC
  TGTCTCATGGAGACTGTAGAGCTGTTGCAAGCAAAGGTGAGCGCCCTAGACCTTGCAGTT
  TTGGATCAAGTGGAGGCTCGGCTACAGAGTGTCCTGGGAAAGGTGAACGAGATTGCCAAG
  CATAAAGCCTCTGTAGAAGATGCAGATACACAAAGCAAGGTGCACCAGCTATATGAAACT
  ATACAGCGCTGGAGCCCCATTGCCTCCACCCTCCCTGAGCTGGTGCAGAGACTTGTCACC
  ATCAAGCAGCTGCACGAGCAAGCCATGCAGTTTGGTCAGCTCCTGACACACTTGGATACC
  ACCCAGCAGATGATTGCTAATTCCTTGAAGGACAATACCACCCTCTTGACCCAGGTGCAG
  ACAACCATGCGTGAAAACCTGGCCACAGTTGAGGGGAACTTTGCCAGCATTGATGAACGG
  ATGAAGAAGCTGGGAAAGTGA
  
  Clone variation with respect to NM_006400.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006400 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTCCCTTTGCCGCCGCC
  TTAGCCCGGGACCCGAACCCAGCCTCTCCCCTACCCGAACACCGGCCCCGGCTCCACCGA
  GGCCCGGGTCCCCCAGCCCGTCTCGCCGCCGCCATGGCGGACCCTAAATACGCCGACCTT
  CCCGGCATTGCCAGGAATGAGCCAGATGTTTATGAAACTAGCGACCTACCTGAGGATGAT
  CAAGCGGAGTTCGATGCGTTTGCACAAGAGCTGGAGGAGCTGACAAGCACAAGTGTGGAA
  CACATCATTGTCAATCCTAATGCTGCCTATGACAAGTTCAAGGACAAGAGAGTGGGGACA
  AAGGGACTTGATTTCTCAGATCGTATTGGAAAAACCAAGAGGACAGGATATGAATCTGGA
  GAATATGAGATGCTTGGAGAGGGTCTGGGAGTGAAGGAGACACCCCAGCAAAAGTACCAG
  CGCCTACTGCATGAGGTCCAAGAGCTGACAACTGAAGTTGAAAAAATCAAGACGACAGTG
  AAGGAGTCAGCCACAGAGGAGAAGCTGACCCCCTGTGTTGCTGGCTAAACAGCTGGCAGC
  CCTGAAGCAGCAGCTGGGTTGCTTCCACCTGGAGAAGCTGCTGGGACCAGATGCTGCAAT
  CAACCTTACCGACCCCGATGGCGCCCTGGCTAAGCGCCTACTACTGCAGCTGGGAAGCAC
  AAGAACAGCAAAGGGGGATCAGGNGGAAAAACACTGGGACCCCCCAGATAGCAGCCTTGT
  CACTTATGAACTACATTCCTCGNCTGAGNCAGGGACAGTCTCTCAAGCTGCCAAAGTCGC
  AAAACTGAAAAGCGCCTGACAGAGCTGAAGACAGCTGTACGTTGGGATCAGGATGCTCAN
  AATCCCCTTCCTGCACGTCACAGGGGACCCTGTCTATGGAGACTGTTNAGCTGTGCAGCA
  AAGTGGACGCCCTAAACTTGCAGTTTTGGACAGTGGNAGCTCGCTTACAATTGCCTGGGA
  AGGGGANANATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006400 unedited
  CGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAGGATACAACAGCATTTA
  ATGGGTCAGAAACAGTTGTACAGTATTACAGTCAGCCACAGAAGCTGTGTTGGGGGACAA
  GACCCAATCCTTCCCCACACCAGGCAAAGCAGTATTGGACATGAGTTGGCATGTGGCTGG
  GCCCACGTCCTTATCCCCCAGGCCTGAGGGGAGACCACCTTCTGATGATAACCAACCCCT
  AGCTACCACTCTGTATTCATCAGGGGAGGGGTATAAACCCCACATGCAAGAAGAACCCTT
  GCCCCCAGTGTCAAATGGGATGGGGATGCTAGAGTTATAGTAAAGGGGAAACCCTATGTA
  AGCTGTTAACAGAGTTCACAGGGGTAGGGATAACCCCTGTTCTCCAGCTCCCAAATGTGC
  TCACTTTCCCAGCTTCTTCATCCGTTCATCAATGCTGGCAAAGTTCCCCTCAACTGTGGC
  CAGGTTTTCACGCATGGTTGTCTGCACCTGGGTCAAGAGGGCGGTATTGTCCTTCAAGGA
  ATTAGCAATCATCTGCTGGGTGGTATCCAAGTGTGTCAGGAGCTGACCAAACTGCATGGC
  TCGCTCGTGCAGCTGCTTGATGGAGACAAGTCTCTGCACCAGCTCAGGGAGGGCGGAGGC
  AATGGGGCCTCAGCGCCGAATAGTCTCATATAGCCGGCGCCCCCTGCTTTGCGTATTTGC
  CTCTTCTACAGAGGCTTTATGCCTTGGCAATCTGGTCCACCTTCCCCAGGACACTCTGTA
  CCCCAGCCTCCACTTGACCAAAACTGCAAGGCTAGGTCGCTCACCTTTGCTTGCAACAAC
  CTTACAGTCCCCTGAGACAGGCTNCCTGTACACCTGCGAAAGGGAGATTTGGACCTTCTG
  AAACAACCGACAAGTGTCTCACACTCTGTAGGGCCCTTTAACTCCCGCACTTGGGACCTG
  GAGAAACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006400
• ORF Size: 1221 bp
• Insert Size: 1710
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006400.3, NP_006391.1
• RefSeq Size: 1757
• RefSeq ORF: 1221
• Locus ID: 10540
• Domains: Dynamitin
• Protein Pathways: Huntington's disease
• Gene Summary: This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.