BAAT (NM_001701) Human Untagged Clone

CAT#: SC112712

BAAT (untagged)-Human bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) (BAAT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAAT
• Synonyms: BACAT; BAT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112712 sequence for NM_001701 edited (data generated by NextGen Sequencing)
  ATGATCCAGTTGACAGCTACCCCTGTGAGTGCACTTGTTGATGAGCCAGTGCATATCCAA
  GCTACAGGCCTGATTCCCTTTCAGATGGTGAGTTTTCAGGCATCACTGGAAGATGAAAAC
  GGAGACATGTTTTATTCTCAAGCCCACTATAGGGCCAATGAATTCGGTGAGGTGGACCTG
  AATCATGCTTCTTCACTTGGAGGGGATTATATGGGAGTCCACCCCATGGGTCTCTTCTGG
  TCTCTGAAACCTGAAAAGCTATTAACAAGACTGTTGAAAAGAGATGTGATGAATAGGCCT
  TTCCAGGTCCAAGTAAAACTTTATGACTTAGAGTTAATAGTGAACAATAAAGTTGCCAGT
  GCTCCAAAGGCCAGCCTGACTTTGGAGAGGTGGTATGTGGCACCTGGTGTCACACGAATT
  AAGGTTCGAGAAGGCCGCCTTCGAGGAGCTCTCTTTCTCCCTCCAGGAGAGGGTCTCTTC
  CCAGGGGTAATTGATTTGTTTGGTGGTTTGGGTGGGCTGCTTGAATTTCGGGCCAGCCTC
  CTAGCCAGTCGTGGCTTCGCCTCCTTGGCCTTGGCTTACCATAACTATGAAGACCTGCCC
  CGCAAACCAGAAGTAACAGATTTGGAATATTTTGAGGAGGCTGCCAACTTTCTCCTGAGA
  CATCCAAAGGTCTTTGGCTCAGGCGTTGGGGTAGTCTCTGTATGTCAAGGAGTACAGATT
  GGACTATCTATGGCTATTTACCTAAAGCAAGTCACAGCCACGGTACTTATTAATGGGACC
  AACTTTCCTTTTGGCATTCCACAGGTATATCATGGTCAGATCCATCAGCCCCTTCCCCAT
  TCTGCACAATTAATATCCACCAATGCCTTGGGGTTACTAGAGCTCTATCGCACTTTTGAG
  ACAACTCAAGTTGGGGCCAGTCAATATTTGTTTCCTATTGAAGAGGCCCAGGGGCAATTC
  CTCTTCATTGTAGGAGAAGGTGATAAGACTATCAACAGCAAAGCACACGCTGAACAAGCC
  ATAGGACAGCTGAAGAGACATGGGAAGAACAACTGGACCCTGCTATCTTACCCTGGGGCA
  GGCCACCTGATAGAACCTCCCTATTCTCCTCTGTGCTGTGCCTCAACGACCCACGATTTG
  AGGTTACACTGGGGAGGAGAGGTGATCCCACACGCAGCTGCACAGGAACATGCTTGGAAG
  GAGATCCAGAGATTTCTCAGGAAGCACCTCATTCCAGATGTGACCAGTCAACTCTAA
  
  Clone variation with respect to NM_001701.3
  59 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001701 unedited
  CGCCAGAATTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACCAGGTGACATCAC
  CCCCGGAGGCCCAGCTGTAAATTCCTCTCTTTGTACTCTTTCTCTTTATTTCTCAGACCA
  GCCGACACTTAGGGAAAATAGAACCTACGCTGAAATTTTGGGGGCAGGTTCTCTTGCTAG
  GTTTTGAGGTTTTGCTGAAGATATTCCTGAAGAATCATCCCAGGTGCCACACTAAAAAAA
  TGATCCAGTTGACAGCTACCCCTGTGAGTGCACTTGTTGATGAGCCAGTGCATATCCAAG
  CTACAGGCCTGATTCCCTTTCAGATGGTGAGTTTTCAGGCATCACTGGAAGATGAAAACG
  GAGACATGTTTTATTCTCAAGCCCACTATAGGGCCAATGAATTCGGTGAGGTGGACCTGA
  ATCATGCTTCTTCACTTGGAGGGGATTATATGGGAGTCCACCCCATGGGTCTCTTCTGGT
  CTCTGAAACCTGAAAAGCTATTAACAAGACTGTTGAAAAGAGATGTGATGAATAGGCCTT
  TCCAGGTCCAAGTAAAACTTTATGACTTAGAGTTAATAGTGAACAATAAAGTTGCCAGTG
  CTCCAAAGGCCAGCCTGACTTTGGAGAGGTGGTATGTGGCACCTGGTGTCACACGAATTA
  AGGTTCGAGAAGGCCGCCTTCGAGGAGCTCTCTTTCTCCCTCCAGGAGAGGGTCTCTTCC
  CAGGGTAATTGATTTGTTTGGTGGTTTAGGGTAGGGCTGCTTGAATTTCAGGCCAGCCTC
  CTAGCCAGTCGTGGCTTCGCCTCCTTGGCCTTGGCTTACCATAACTATGAAGACCTGCCC
  CGCAACCAGAAGTACAGATTTGGAATATTCTTGAGGAGCTGCCAACTTTCTCCTGAGACA
  NCCAAAGGTCTTAGGCTCANGCGNTGGAGGTAGTCTCTGTATGTCAAGGNAGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001701 unedited
  NTTTTTTNNNNNNNNNTTATCTATGNACCGCGGNCCGCAATCTANGATCGAGTTTTTTTT
  TTTTTTTTTTACTTTTATTAATTTTATTTTTAATACAGATTTTCAGTAAGGGGCATTTTC
  AACCTAATTGGTTCTATTTTCTTGTATTTTCCATTTTAATTTGCTTCATAACTTAAACCA
  AGGCTCTTCCAGTCTTAGTTATTATGTCTCAGTTATGTGCCAATGGGCATGTTTTTAAGA
  ACTGAAGAGGTAATTTATTGCAATGAACTAACTGACCTCCTCCATTCCTTCTTTCCTTTT
  TGACATGAATTTTACTACCCCACAAATGAAAAATGATGTTGCAAAGTTACTGTGGTGAAG
  TTGAAAAATATCACTAAAATGATTATAATTTAGTATTAATTTTCTCCTGCTGCTCTAATC
  TGATAAATTCTTACTATATAGTGTTTTCTGACATGGTATTTGGTTTTATATCTGTTACTT
  TGGGGCTATTNNCTGTTGCCTTTTCTTATTTTTGCCAGTGTACTATACCTCAGTCCTATT
  TAGAAGACCTGATGGNAAGAAAAGTCATGTAAATATAAAGTAAATGATTTGGTTTATGAT
  TTATCACCATGTCCAGTTTGGNTAAGCTGGTATGCAGAAAAGTGAAAATCAGGTTTTACC
  CTATGCTTTTTTAATATTTAAAATAATCAAATGTGTTGTGGCAGCTGGGGGAACCTCCCG
  CCTGAAAATGAGAAAAGGCCTGGTAAGAGATTTGCGTCCTTATTTTCTAGGATATCTAGN
  CTTCTAGAGNTGACTGGCACATCTGGATGGAGGGCTTTCTGAGAATTCCTTGGATTNCTT
  TCAGCATGTCCTGTCAGCTGCTGGGGGATCACTCTCTCCCAGTGTACTCAATCGTGGGTC
  GTGAGGCCAGACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001701
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001701.2, NP_001692.1
• RefSeq Size: 3108 bp
• RefSeq ORF: 1257 bp
• Locus ID: 570
• Cytogenetics: 9q31.1
• Domains: Bile_Hydr_Trans
• Protein Pathways: Biosynthesis of unsaturated fatty acids, Metabolic pathways, Primary bile acid biosynthesis, Taurine and hypotaurine metabolism
• Gene Summary: 'The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.