Caspase 1 (CASP1) (NM_033295) Human Untagged Clone

CAT#: SC112763

CASP1 (untagged)-Human caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) (CASP1), transcript variant epsilon


  "NM_033295" in other vectors (4)

Reconstitution Protocol

USD 420.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CASP1
Synonyms ICE; IL1BC; P45
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_033295, the custom clone sequence may differ by one or more nucleotides


ATGGCCGACAAGGTCCTGAAGGAGAAGAGAAAGCTGTTTATCCGTTCCATGGGTGAAGATAATGTTTCTT
GGAGACATCCCACAATGGGCTCTGTTTTTATTGGAAGACTCATTGAACATATGCAAGAATATGCCTGTTC
CTGTGATGTGGAGGAAATTTTCCGCAAGGTTCGATTTTCATTTGAGCAGCCAGATGGTAGAGCGCAGATG
CCCACCACTGAAAGAGTGACTTTGACAAGATGTTTCTACCTCTTCCCAGGACATTAA


>OriGene 5' read for NM_033295 unedited
GATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAGTAGGAGTTT
CTGGAAACCTATCTTTACCAACTACAGAAGAGTTTGAGGATGATGCTATTAAGAAAGCCC
ACATAGAGAAGGATTTTATCGCTTTCTGCTCTTCCACACCAGATAATGTTTCTTGGAGAC
ATCCCACAATGGGCTCTGTTTTTATTGGAAGACTCATTGAACATATGCAAGAATATGCCT
GTTCCTGTGATGTGGAGGAAATTTTCCGCAAGGTTCGATTTTCATTTGAGCAGCCAGATG
GTAGAGCGCAGATGCCCACCACTGAAAGAGTGACTTTGACAAGATGTTTCTACCTCTTCC
CAGGACATTAAAATAAGGAAACTGTATGAATGTCTGTGGGCAGGTACATGTGTATGGTCG
GGAGTGTGGGAAGGTTGAGGAAAGGGTACTGAAAGTCCATTTGAGTCAAGAACTCTAGGT
TTACAGGCTGAGAATCCTTAATCCAAAAATTTGAATTTTGAAATGCTCTAAAATCCAACA
CTGTGTGAGCGCCCACATGATATTCAAAGGAAATGTTTATTGAAACATTTCAAATTATAG
GTTTTTGGATTAGGGATGCTAAACCAGTAAGTATACAGCTGAATTCCAATATACAAAAAT
ATCTGAAATTTGAAATACTTCTGGTAGCATGCATTNTGGATAAGGGATAATCAAGCCATA
TACANGAAATACTGAAGTAATGTCTTTCTTCTGGTCAGTGCAGAGCACGTTGCTCTTCTC
CCAAGTTTTTCATTTAGACCCCTTTTGAGATCATCTGATATTGGCTAGAAGTGCCGTAGA
CTACAACCCTCACTTTTGATGTTTCTCTCCTTCCCTCAGCAGGNTATATTAAACATAGCA
TGTGGNNCTTCCTTTTAAAATGTGTATGNTCCCCATGGTGGNNNATACTTATACTNGCAT
GTCTTTACAAAATCCTTTNCTTGTTTA
Restriction Sites NotI-NotI     
ACCN NM_033295
Insert Size 1650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033295.1, NP_150637.1
RefSeq Size 443 bp
RefSeq ORF 267 bp
Locus ID 834
Cytogenetics 11q22.3
Protein Families Druggable Genome, Protease
Protein Pathways Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
Gene Summary 'This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]'
Transcript Variant: This variant (epsilon) has multiple differences in the coding region but the translation remains in frame, compared to variant alpha. Variant epsilon encodes a protein lacking an internal segment, compared to isoform alpha. Isoform epsilon lacks apoptosis-inducing activity. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.