CD3G (NM_000073) Human Untagged Clone

CAT#: SC112775

CD3G (untagged)-Human CD3g molecule, gamma (CD3-TCR complex) (CD3G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD3G
• Synonyms: CD3-GAMMA; IMD17; T3G
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112775 sequence for NM_000073 edited (data generated by NextGen Sequencing)
  ATGGAACAGGGGAAGGGCCTGGCTGTCCTCATCCTGGCTATCATTCTTCTTCAAGGTACT
  TTGGCCCAGTCAATCAAAGGAAACCACTTGGTTAAGGTGTATGACTATCAAGAAGATGGT
  TCGGTACTTCTGACTTGTGATGCAGAAGCCAAAAATATCACATGGTTTAAAGATGGGAAG
  ATGATCGGCTTCCTAACTGAAGATAAAAAAAAATGGAATCTGGGAAGTAATGCCAAGGAC
  CCTCGAGGGATGTATCAGTGTAAAGGATCACAGAACAAGTCAAAACCACTCCAAGTGTAT
  TACAGAATGTGTCAGAACTGCATTGAACTAAATGCAGCCACCATATCTGGCTTTCTCTTT
  GCTGAAATCGTCAGCATTTTCGTCCTTGCTGTTGGGGTCTACTTCATTGCTGGACAGGAT
  GGAGTTCGCCAGTCGAGAGCTTCAGACAAGCAGACTCTGTTGCCCAATGACCAGCTCTAC
  CAGCCCCTCAAGGATCGAGAAGATGACCAGTACAGCCACCTTCAAGGAAACCAGTTGAGG
  AGGAATTGA
  
  Clone variation with respect to NM_000073.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000073 unedited
  NGGTTCGGGATTAGTATCCGATTCATNATAGGGCGGCCGCGAATTCGGCACCAGGGCTGC
  TAAGGGCTGCTCCACGCTTTTGCNCGGAGACAGAGACTGACATGGAACAGGGGAAGGGCC
  TGGCTGTCCTCATCCTGGCTATCATTCTTCTTCAAGTACTTTGGCCCAGTCAATCAAAGG
  AAACCACTTGGTTAAGGTGTATGACTATCAAGAAGATGGTTCGGTACTTCTGACTTGTGA
  TGCAGAAGCCAAAAATATCACATGGTTTAAAGATGGGAAGATGATCGGCTTCCTAACTGA
  AGATAAAAAAAAATGGAATCTGGGAAGTAATGCCAAGGACCCTCGAGGGATGTATCAGTG
  TAAAGGATCACAGAACAAGTCAAAACCACTCCAAGTGTATTACAGAATGTGTCAGAACTG
  CATTGAACTAAATGCAGCCACCATATCTGGCTTTCTCTTTGCTGAAATCGTCAGCATTTT
  CGTCCTTGCTGTTGGGGTCTACTTCATTGCTGGACAGGATGGAGTTCGCCAGTCGAGAGC
  TTCAGACAAGCAGACTCTGTTGCCCAATGACCAGCTCTACCAGCCCCTCAAGGATCGAGA
  AGATGACCAGTACAGCCACCTTCAAGGAAACCAGTTGAGGAGGAATTGAACTCAGGACTC
  AGAGTAGTCCAGGTGTTCTCCTCCTATTCAGTTCCCAGAATCAAAAGCATGCATTTTGGA
  AAGCTCCTAGCAGAGAGACTTTCAGCCCTAAATCTAGACTCAAGGTTCCCAGAGATGACA
  AATGGAGAAGAAAGGCCATCAGAGCAAATTTGGGGTTTCTCAAATAATAAAATAAAACCA
  ATCCTGTGTTTCAGAAGCGCACCTATTGNGGAAAATGTNNAAGAAAATGAAAGTTCCATA
  ACCCCCTGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000073
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000073.1, NP_000064.1
• RefSeq Size: 822 bp
• RefSeq ORF: 549 bp
• Locus ID: 917
• Cytogenetics: 11q23.3
• Domains: ITAM, IGc2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, T cell receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]'