TLR5 (NM_003268) Human Untagged Clone

CAT#: SC112789

TLR5 (untagged)-Human toll-like receptor 5 (TLR5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLR5
• Synonyms: MELIOS; SLE1; SLEB1; TIL3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003268 edited
  CCTATAGTCTCAGGAACTGAAGTCTTTAAACAACCAGGGACCCTCTGCCCCTAGAATAAG
  AACATACTAGAAGTCCCTTCTGCTAGGACAACGAGGATCATGGGAGACCACCTGGACCTT
  CTCCTAGGAGTGGTGCTCATGGCCGGTCCTGTGTTTGGAATTCCTTCCTGCTCCTTTGAT
  GGCCGAATAGCCTTTTATCGTTTCTGCAACCTCACCCAGGTCCCCCAGGTCCTCAACACC
  ACTGAGAGGCTCCTGCTGAGCTTCAACTATATCAGGACAGTCACTGCTTCATCCTTCCCC
  TTTCTGGAACAGCTGCAGCTGCTGGAGCTCGGGAGCCAGTATACCCCCTTGACTATTGAC
  AAGGAGGCCTTCAGAAACCTGCCCAACCTTAGAATCTTGGACCTGGGAAGTAGTAAGATA
  TACTTCTTGCATCCAGATGCTTTTCAGGGACTGTTCCATCTGTTTGAACTTAGACTGTAT
  TTCTGTGGTCTCTCTGATGCTGTATTGAAAGATGGTTATTTCAGAAATTTAAAGGCTTTA
  ACTCGCTTGGATCTATCCAAAAATCAGATTCGTAGCCTTTACCTTCATCCTTCATTTGGG
  AAGTTGAATTCCTTAAAGTCCATAGATTTTTCCTCCAACCAAATATTCCTTGTATGTGAA
  CATGAGCTCGAGCCCCTACAAGGGAAAACGCTCTCCTTTTTTAGCCTCGCAGCTAATAGC
  TTGTATAGCAGAGTCTCAGTGGACTGGGGAAAATGTATGAACCCATTCAGAAACATGGTG
  CTGGAGATACTAGATGTTTCTGGAAATGGCTGGACAGTGGACATCACAGGAAACTTTAGC
  AATGCCATCAGCAAAAGCCAGGCCTTCTCTTTGATTCTTGCCCACCACATCATGGGTGCC
  GGGTTTGGCTTCCATAACATCAAAGATCCTGACCAGAACACATTTGCTGGCCTGGCCAGA
  AGTTCAGTGAGACACCTGGATCTTTCACATGGGTTTGTCTTCTCCCTGAACTCACGAGTC
  TTTGAGACACTCAAGGATTTGAAGGTTCTGAACCTTGCCTACAACAAGATAAATAAGATT
  GCAGATGAAGCATTTTACGGACTTGACAACCTCCAAGTTCTCAATTTGTCATATAACCTT
  CTGGGGGAACTTTACAGTTCGAATTTCTATGGACTACCTAAGGTAGCCTACATTGATTTG
  CAAAAGAATCACATTGCAATAATTCAAGACCAAACATTCAAATTCCTGGAAAAATTACAG
  ACCTTGGATCTCCGAGACAATGCTCTTACAACCATTCATTTTATTCCAAGCATACCCGAT
  ATCTTCTTGAGTGGCAATAAACTAGTGACTTTGCCAAAGATCAACCTTACAGCGAACCTC
  ATCCACTTATCAGAAAACAGGCTAGAAAATCTAGATATTCTCTACTTTCTCCTACGGGTA
  CCTCATCTCCAGATTCTCATTTTAAATCAAAATCGCTTCTCCTCCTGTAGTGGAGATCAA
  ACCCCTTCAGAGAATCCCAGCTTAGAACAGCTTTTCCTTGGAGAAAATATGTTGCAACTT
  GCCTGGGAAACTGAGCTCTGTTGGGATGTTTTTGAGGGACTTTCTCATCTTCAAGTTCTG
  TATTTGAATCATAACTATCTTAATTCCCTTCCACCAGGAGTATTTAGCCATCTGACTGCA
  TTAAGGGGACTAAGCCTCAACTCCAACAGGCTGACAGTTCTTTCTCACAATGATTTACCT
  GCTAATTTAGAGATCCTGGACATATCCAGGAACCAGCTCCTAGCTCCTAATCCTGATGTA
  TTTGTATCACTTAGTGTCTTGGATATAACTCATAACAAGTTCATTTGTGAATGTGAACTT
  AGCACTTTTATCAATTGGCTTAATCACACCAATGTCACTATAGCTGGGCCTCCTGCAGAC
  ATATATTGTGTGTACCCTGACTCGCTCTCTGGGGTTTCCCTCTTCTCTCTTTCCACGGAA
  GGTTGTGATGAAGAGGAAGTCTTAAAGTCCCTAAAGTTCTCCCTTTTCATTGTATGCACT
  GTCACTCTGACTCTGTTCCTCATGACCATCCTCACAGTCACAAAGTTCCGGGGCTTCTGT
  TTTATCTGTTATAAGACAGCCCAGAGACTGGTGTTCAAGGACCATCCCCAGGGCACAGAA
  CCTGATATGTACAAATATGATGCCTATTTGTGCTTCAGCAGCAAAGACTTCACATGGGTG
  CAGAATGCTTTGCTCAAACACCTGGACACTCAATACAGTGACCAAAACAGATTCAACCTG
  TGCTTTGAAGAAAGAGACTTTGTCCCAGGAGAAAACCGCATTGCCAATATCCAGGATGCC
  ATCTGGAACAGTAGAAAGATCGTTTGTCTTGTGAGCAGACACTTCCTTAGAGATGGCTGG
  TGCCTTGAAGCCTTCAGTTATGCCCAGGGCAGGTGCTTATCTGACCTTAACAGTGCTCTC
  ATCATGGTGGTGGTTGGGTCCTTGTCCCAGTACCAGTTGATGAAACATCAATCCATCAGA
  GGCTTTGTACAGAAACAGCAGTATTTGAGGTGGCCTGAGGATCTCCAGGATGTTGGCTGG
  TTTCTTCATAAACTCTCTCAACAGATACTAAAGAAAGAAAAAGAAAAGAAGAAAGACAAT
  AACATTCCGTTGCAAACTGTAGCAACCATCTCCTAATCAAAGGAGCAATTTCCAACTTAT
  CTCAAGCCACAAATAACTCTTCACTTTGTATTTGCACCAAGTTATCATTTTGGGGTCCTC
  TCTGGAGGTTTTTTTT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003268 unedited
  GGATTTTGGTATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCCCAGAGTCT
  CACTCTGCCGCCCAGGCTGGACTGCAGTGACACAATCTCGGCTGACTGCAACCACTGCCT
  CCAGGGTTCAAGCGATTCTCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGATTGATG
  TTCATGTTCCTGACACTACTACAAGATTCATACTCCTGATGCTACTGACAACGTGGCTTC
  TCCACAGTCACCAAACCAGGGATGCTATACTGGACTTCCCTACTCTCATCTGCTCCAGCC
  CCCTGACCTTATAGTTGCCCAGCTTTCCTGGCAATTGACTTTGCCCATCAATACACAGGA
  TTTAGCATCCAGGGAAGATGTCGGAGCCTCAGATGTTAATTTTCTAATTGAGAATGTTGG
  CGCTGTCCGAACCTGGAGACAGAGTATCAGCGCCTTTGCTTGCTGCTGTTTTTGCTGTTT
  TTTGATGCTGGGAACCACCACCTAAAGATAGTAAAGAAAACACAGGAAGCTTTCCGGAAA
  ACAAAAAGTCCTTTCTCCTGATTCACCAAAAAATAAAATACTGACTACCATCACTGTGAT
  GAGATTCCTATAGTCTCAGGAACTGAAGTCTTTAAACAACCAGGGACCCTCTGCCCCTAG
  AATAAGAACATACTAGAAGTCCCTTCTGCTAGGACAACGAGGATCATGGGAGACCACCTG
  GACCTTCTCCTAGGAGTGGTGCTCATGGCCGGTCCTGTGTTTGGGATTCCTTCCTGCTCC
  TTTGATGGCCGAATAGCCTTNTATCGNTTCTGNCACCTCNACCCAGTCCCNCCAGTCCTC
  ACACCACTGAGAGGCTCCTGCTGAGCTTCACTATATCCAGACAGTCACTGCTTCATCCTT
  CCCTTTTCTGNACAGCTGCAGCTGCTGGAGCTCGGNAGCCATATACCCCCTTGACTATGC
  ACAGGNNAGGCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003268 unedited
  GCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGCAGTTGCAATTTTCT
  AGATCTATTATTTTCCATTTGGAGGTTAGTGAGACAGAACATGGTGTTGATACGAAAATT
  GAGAGATTTATGTTGTTTTCATAGTAGCAAAAAGAAAAAAAAAACCTCCAGAGAGGACCC
  CAAAATGATAACTTGGTGCAAATACAAAGTGAAGAGTTATTTGTGGCTTGAGATAAGTTG
  GAAATTGCTCCTTTGATTAGGAGATGGTTGCTACAGTTTGCAACGGAATGTTATTGGCTT
  TCTTCTTTTCTTTTTCTTTCTTTAGTATCTGTTGAGAGAGTTTATGAAGAAACCAGCCAA
  CATCCTGGAGATCCTCAGGCCACCTCAAATACTGCTGTTTCTGTACAAAGCCTCTGATGG
  ATTGATGTTTCATCAACTGGTACTGGGACAAGGACCCAACCACCACCATGATGAGAGCAC
  TGTTAAGGTCAGATAAGCACCTGCCCTGGGCATAACTGAAGGCTTCAAGGCACCAGCCAT
  CTCTAAGGAAGTGTCTGCTCACAAGACAAACGATCTTTCTACTGTTCCAGATGGCATCCT
  GGATATTGGCAATGCGGTTTTCTCCTGGGACAAAGTCTCTTTCTTCAAAGCACAGGTTGA
  ATCTGTTTTGGTCACTGTATTGAGTGTCCAGGTGTTTGAGCAAGCATTCTGCACCCATGT
  GAAGTCTTTGCTGCTNGAGCACAATAGGCATCATATTTGTACTATCANGGNTCTGTGCCC
  TGGGGATGGTCTTTGACACCAGTCTCTGGGCTGTCTTATACAAATAAACAGAGCCCCGGG
  ACTTTGTGACTGTGAGGATGGTCATGAGGAACAGAGTCANATGACAGTGCATACATGAAA
  AGGAGAACTTTAGACTTTAAGACTTCTCTCATACAACCTTCGGGAAAGAGAAAAGGAACC
  CCNAGGCGGTCGGGTCCCCAATATGTTGGAGGAGCCCCCTTATGACATGGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003268
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003268.3, NP_003259.2
• RefSeq Size: 3369 bp
• RefSeq ORF: 2577 bp
• Locus ID: 7100
• Cytogenetics: 1q41
• Domains: TIR, LRRCT, LRR, LRR_TYP, LRR_SD22, LRR_PS
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]'