POLR2H (NM_006232) Human Untagged Clone

CAT#: SC112792

POLR2H (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2H
• Synonyms: RPABC3; RPB8; RPB17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006232 edited
  ATGGCGGGCATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAG
  AAGTTTGACCGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATC
  TTAGATGTAAACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATA
  GCTAGTACCTTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGAT
  AGGCCTTCCAGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAG
  GGAGATGAAACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGC
  CTGCTCATGAGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGA
  GTTTATCTCCTGATGAAGAAGCTAGCCTTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006232 unedited
  GGTTCACATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCGC
  TTTCAGGAGGTGCTTTTGGTTCTCTCCGGTCTTGTCCACGCTAGGGGGTGCACGTACTCC
  CAACTGTGGTCGCGCTCTCACCCCTTCTGCTGCTCTCGTGGCCCCCTCGCGATGGCGGGC
  ATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAGAAGTTTGAC
  CGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATCTTAGATGTA
  AACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATAGCTAGTACC
  TTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGATAGGCCTTCC
  AGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAGGGAGATGAA
  ACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGCCTGCTCATG
  AGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGAGTTTATCTC
  CTGATGAAGAAGCTAGCCTTCTGAACCTCGCCTGAAGCCAGCCTCTCTGCCAAGTCACTC
  AGGTCATGGGCATTGTTCAAGCCTGAGTGGCAGCCGCTCTTGCTCACCTGTTGAGGAAGG
  GCTGGCTCACTGTCCACCGTGGCGGCATCTTTAACTGGCCTCCACTCAATGGGAAACTGA
  CTCGCCTGTGAAAGACACAGTNGGAGAGCTGCAAATGAATCAGAAGCTNTATGTATATGA
  TTTTTAATTAAACTTTACTTTTTCAGAANAAAAACAAAAAAACTCGACTCTAGATTGCGG
  CCGCGGTCATAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006232 unedited
  NAGCTTGTACCCGGCCGCAATCNANGATCGGTTTTTTTTTTTTTTTTTTCTGAAAAGTAA
  AGTTTAATTTAAAAATCATATACATAAAGCTTCTGATTCATTTTCAGCTCTCCCACTGTG
  TCTTTCACAGGCGAGTCAGTTTCCCATTGAGTGGAGGCCAGTTAAAGATGCCGCCACGGT
  GGACAGTGAGCCAGCCCTTCCTCAACAGGTGAGCATGATCGGCTGCCACTCAGGCTTGAA
  CAATGCCCATGACCTGAGTGACTTGGCAGAGAGGCTGGCTTCAGGCGAGGTTCAGAAGGC
  TAGCTTCTTCATCAGGAGATAAACTCTGGAGTCCACCTCGAATCCATGCAGGTTGTTGGC
  ATCCCCCTGCAGCCTCATGAGCAGGCCCCCATAGGACACGTACGCAGAGAGGCGTGTTGC
  TGCTTCAGTAGAAGTTTCATCTCCCTCAATCCTGTACACTTTTCCATACATTACATACTC
  AAACTGGTCAGCCCTGGAAGGCCTATCATCAGTGGGGTTGTATTCACCATCATCCAGGGT
  TCCATCTTCCTACAAGGTACTAGCTATGACCAACCGAAACTTGTCCCCCAAGTCTACAGG
  GTAAATTTGAATGTTTACCTCCTAGATTAGACCCATCTTGAAAGATTCACTCTTACCTTG
  CAGCCCAAACCCTTCGTCAACCTTTGGCCCTCCGGGCCATTTCCCTCCCTTGGAAATTTC
  CCCAAACGGCTGCCCGGCCTCTCGAGGGGGCCCCCTTACCCCCTCAGGGGTGAGACGCCG
  CCCCCGCTGGGGAATCCCCCCCCCCTTCCGTGGTCCAACTGGTGGGACCCTTCCCCCCTC
  TGTAACCCCCCCTTTGGTCGCTTTCCCGGCCGTTTTTTCGGGTCCCGTTCCATATTTTGT
  GGTCCCCTTTTGTGTCCTTGTGTTTCTATCATACTTACGCCACAACAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006232
• Insert Size: 840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006232.2, NP_006223.2
• RefSeq Size: 821 bp
• RefSeq ORF: 453 bp
• Locus ID: 5437
• Cytogenetics: 3q27.1
• Domains: RNA_pol_Rpb8
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.