POLR2H (NM_006232) Human Untagged Clone

CAT#: SC112792

POLR2H (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H)


  "NM_006232" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol POLR2H
Synonyms RPABC3; RPB8; RPB17
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_006232 edited
ATGGCGGGCATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAG
AAGTTTGACCGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATC
TTAGATGTAAACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATA
GCTAGTACCTTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGAT
AGGCCTTCCAGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAG
GGAGATGAAACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGC
CTGCTCATGAGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGA
GTTTATCTCCTGATGAAGAAGCTAGCCTTCTGA
>OriGene 5' read for NM_006232 unedited
GGTTCACATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCGC
TTTCAGGAGGTGCTTTTGGTTCTCTCCGGTCTTGTCCACGCTAGGGGGTGCACGTACTCC
CAACTGTGGTCGCGCTCTCACCCCTTCTGCTGCTCTCGTGGCCCCCTCGCGATGGCGGGC
ATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAGAAGTTTGAC
CGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATCTTAGATGTA
AACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATAGCTAGTACC
TTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGATAGGCCTTCC
AGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAGGGAGATGAA
ACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGCCTGCTCATG
AGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGAGTTTATCTC
CTGATGAAGAAGCTAGCCTTCTGAACCTCGCCTGAAGCCAGCCTCTCTGCCAAGTCACTC
AGGTCATGGGCATTGTTCAAGCCTGAGTGGCAGCCGCTCTTGCTCACCTGTTGAGGAAGG
GCTGGCTCACTGTCCACCGTGGCGGCATCTTTAACTGGCCTCCACTCAATGGGAAACTGA
CTCGCCTGTGAAAGACACAGTNGGAGAGCTGCAAATGAATCAGAAGCTNTATGTATATGA
TTTTTAATTAAACTTTACTTTTTCAGAANAAAAACAAAAAAACTCGACTCTAGATTGCGG
CCGCGGTCATAN
>OriGene 3' read for NM_006232 unedited
NAGCTTGTACCCGGCCGCAATCNANGATCGGTTTTTTTTTTTTTTTTTTCTGAAAAGTAA
AGTTTAATTTAAAAATCATATACATAAAGCTTCTGATTCATTTTCAGCTCTCCCACTGTG
TCTTTCACAGGCGAGTCAGTTTCCCATTGAGTGGAGGCCAGTTAAAGATGCCGCCACGGT
GGACAGTGAGCCAGCCCTTCCTCAACAGGTGAGCATGATCGGCTGCCACTCAGGCTTGAA
CAATGCCCATGACCTGAGTGACTTGGCAGAGAGGCTGGCTTCAGGCGAGGTTCAGAAGGC
TAGCTTCTTCATCAGGAGATAAACTCTGGAGTCCACCTCGAATCCATGCAGGTTGTTGGC
ATCCCCCTGCAGCCTCATGAGCAGGCCCCCATAGGACACGTACGCAGAGAGGCGTGTTGC
TGCTTCAGTAGAAGTTTCATCTCCCTCAATCCTGTACACTTTTCCATACATTACATACTC
AAACTGGTCAGCCCTGGAAGGCCTATCATCAGTGGGGTTGTATTCACCATCATCCAGGGT
TCCATCTTCCTACAAGGTACTAGCTATGACCAACCGAAACTTGTCCCCCAAGTCTACAGG
GTAAATTTGAATGTTTACCTCCTAGATTAGACCCATCTTGAAAGATTCACTCTTACCTTG
CAGCCCAAACCCTTCGTCAACCTTTGGCCCTCCGGGCCATTTCCCTCCCTTGGAAATTTC
CCCAAACGGCTGCCCGGCCTCTCGAGGGGGCCCCCTTACCCCCTCAGGGGTGAGACGCCG
CCCCCGCTGGGGAATCCCCCCCCCCTTCCGTGGTCCAACTGGTGGGACCCTTCCCCCCTC
TGTAACCCCCCCTTTGGTCGCTTTCCCGGCCGTTTTTTCGGGTCCCGTTCCATATTTTGT
GGTCCCCTTTTGTGTCCTTGTGTTTCTATCATACTTACGCCACAACAAA
Restriction Sites NotI-NotI     
ACCN NM_006232
Insert Size 840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006232.2, NP_006223.2
RefSeq Size 821 bp
RefSeq ORF 453 bp
Locus ID 5437
Cytogenetics 3q27.1
Domains RNA_pol_Rpb8
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary 'The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]'
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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