SLC5A7 (NM_021815) Human Untagged Clone

CAT#: SC112868

SLC5A7 (untagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC5A7
• Synonyms: CHT; CHT1; CMS20; HMN7A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112868 sequence for NM_021815 edited (data generated by NextGen Sequencing)
  ATGGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTG
  GTTGGAATATGGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAA
  GCCATCATAGTTGGTGGCCGAGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCT
  ACCTGGGTCGGAGGAGGGTATATCAATGGCACAGCTGAAGCAGTTTATGTACCAGGTTAT
  GGCCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTAGTCTGATTTTAGGTGGCCTG
  TTCTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCCGTTTCAGCAA
  ATCTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTC
  TGGGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGAT
  ATGCACATTTCTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGGGAGGG
  CTCTATTCTGTGGCCTACACTGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGG
  ATCAGCGTCCCCTTTGCATTGTCACATCCTGCAGTCGCAGACATCGGGTTCACTGCTGTG
  CATGCCAAATACCAAAAGCCGTGGCTGGGAACTGTTGACTCATCTGAAGTCTACTCTTGG
  CTTGATAGTTTTCTGTTGTTGATGCTGGGTGGAATCCCATGGCAAGCATACTTTCAGAGG
  GTTCTCTCTTCTTCCTCAGCCACCTATGCTCAAGTGCTGTCCTTCCTGGCAGCTTTCGGG
  TGCCTGGTGATGGCCATCCCAGCCATACTCATTGGGGCCATTGGAGCATCAACAGACTGG
  AACCAGACTGCATATGGGCTTCCAGATCCCAAGACTACAGAAGAGGCAGACATGATTTTA
  CCAATTGTTCTGCAGTATCTCTGCCCTGTGTATATTTCTTTCTTTGGTCTTGGTGCAGTT
  TCTGCTGCTGTTATGTCATCAGCAGATTCTTCCATCTTGTCAGCAAGTTCCATGTTTGCA
  CGGAACATCTACCAGCTTTCCTTCAGACAAAATGCTTCGGACAAAGAAATCGTTTGGGTT
  ATGCGAATCACAGTGTTTGTGTTTGGAGCATCTGCAACAGCCATGGCCTTGCTGACGAAA
  ACTGTGTATGGGCTCTGGTACCTCAGTTCTGACCTTGTTTACATCGTTATCTTCCCCCAG
  CTGCTTTGTGTACTCTTTGTTAAGGGAACCAACACCTATGGGGCCGTGGCAGGTTATGTT
  TCTGGCCTCTTCCTGAGAATAACTGGAGGGGAGCCATATCTGTATCTTCAGCCCTTGATC
  TTCTACCCTGGCTATTACCCTGATGATAATGGTATATATAATCAGAAATTTCCATTTAAA
  ACACTTGCCATGGTTACATCATTCTTAACCAACATTTGCATCTCCTATCTAGCCAAGTAT
  CTATTTGAAAGTGGAACCTTGCCACCTAAATTAGATGTATTTGATGCTGTTGTTGCAAGA
  CACAGTGAAGAAAACATGGATAAGACAATTCTTGTCAAAAATGAAAATATTAAATTAGAT
  GAACTTGCACTTGTGAAGCCACGACAGAGCATGACCCTCAGCTCAACTTTCACCAATAAA
  GAGGCCTTCCTTGATGTTGATTCCAGTCCAGAAGGGTCTGGGACTGAAGATAATTTACAG
  TGA
  
  Clone variation with respect to NM_021815.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021815 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTCGCGTGCAGCCACC
  ACTCCAGAAGACTTAATGAAGTAGCCAGCTGCAGAAGAATCTGGATCATTAGATAAAAAT
  GGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTGGT
  TGGAATATGGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAAGC
  CATCATAGTTGGTGGCCGAGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCTAC
  CTGGGTCGGAGGAGGGTATATCAATGGCACAGCTGAAGCAGTTTATGTACCAGGTTATGG
  CCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTAGTCTGATTTTAGGTGGCCTGTT
  CTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCCGTTTCAGCAAAT
  CTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTCTG
  GGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGATAT
  GCACATTTCTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGNGGAGGGC
  TCTATTCTGTGGCCTACACTGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGGA
  TCAGCGTNCCCTTTGCATGTCACATCCTGCAGTCGAGACATCGGGTTCACTGCTGTGCAT
  GCCAATACCAAAGCCGTGCTGGNGAACTGTGACTCTCTGAAGNCTACTCTGCTTGAAAGN
  TTTCTGTGGTGAAGCTGGGGGGAATCCTGCAAGCTACTTTTTAAAGGTTTTTTTTTTTCT
  CAGCACTAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021815 unedited
  TCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  AATTNAAAGCATAATTTTATATAATAATTTGACAAACAAAAATATTTGTTTTAAAACCAT
  TATTTGCCTTTGTCTTTGAAGTACCAAACGTCCAATAAATTCCCCCTCTTCCCCGGGTAA
  ATTTACCTGAAGGCTAAACCAAAAAAAAAAATCTTAATTTATGTTAAAAATATAAGGGTA
  ATTTATTATTCCCAAGTAAAAAAAGGAATACCCCTTTTATTAAATTCACTTATTTTAAGC
  TTATATTCGTTTCATAGGCACATAAAATTCATTACTTGTAAGGGTACCAATGAAGTTGTA
  TTTTGATGCTGTTTGAAACAACTTGTTGTTGATATTGCAGTCCACCTACAAGCTTGCTGA
  TTTGAATATGTGGGGGAAAACAAACCTTTTAAATTCCTCAAGAAATATGTTCCATTCTTA
  TCAAAAGTTTGAAAATAAAATATAGGTTTTCTTCTAATGTTTTATCCACAGGTGTTGCCT
  TCCCAAATAAAAAAGCCATTGGCAAAAATGTTCCGTTATTTTTTTNTTCTTCTGAGGGCA
  AATTTTGAAAATCAGGCTTACTGGATTGAACCTGGAAAAACCTGTAAATGTCTGCTTTAC
  TTATTTTTGTAAACCACAGCCATTGAGGGGCCAAAGTATCCTTTTCGCCATTTAACAACT
  TTGGGAACACCTGTTTGTGAAAATGGGGTGAAATCTATGCCCAAGTGGCTAAGTAAAACC
  TTGGGCCATTGTTTCCTCCTTGCATTTTAAATTTTTGACTTAAAAACATTTGCCAACTTA
  ACCTTTTGGGGATAAAACAACTACCTTTGTTACCACTTTGGGGGCGGGAGGAAAAGGGGC
  AACACCCAAATTTTTTTGGGTCAAATTTAAGTTTAGTTTAGCCTTGAACGGAAATTTTCC
  TGTTTTGTTTGTGAAATGATACCGGAAAATTTCTGGAACTTTTTCCCTGAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021815
• ORF Size: 1743 bp
• Insert Size: 5310
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_021815.2, NP_068587.1
• RefSeq Size: 5158
• RefSeq ORF: 1743
• Locus ID: 60482
• Domains: SSF
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.