C3IP1 (KLHL12) (NM_021633) Human Untagged Clone

CAT#: SC112907

KLHL12 (untagged)-Human kelch-like 12 (Drosophila) (KLHL12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLHL12
• Synonyms: C3IP1; DKIR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112907 sequence for NM_021633 edited (data generated by NextGen Sequencing)
  ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTC
  AATTCAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAG
  CAGAAAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCC
  ATGTTCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACT
  GCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTG
  GAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAA
  GCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT
  TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAG
  CATTTTCCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAA
  AAGCTAATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTC
  ATCAACTGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAG
  TATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCT
  TTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTG
  AGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAAT
  GAAGTGCTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAG
  AAATATGACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGT
  TATGTGGCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGT
  TCCCGCCTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTAT
  TCTGTGGCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATG
  ATCTATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGAT
  CCAAACATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGA
  CTCGTAGTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTA
  AATTCAGTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCC
  ACCAAGCGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGA
  TTTGATGGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGG
  ACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGG
  AGACTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTAT
  GACCCTATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCT
  GGTGTTTGTGTTCTCCGCGAGAAGTGA
  
  Clone variation with respect to NM_021633.2
  1230 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021633 unedited
  AGGGCGGGCCGCGAATTCGCACGAGGGTCGCGCGGCCGCAGGGCCAGACCCGGACGGCTC
  CAGAGCCTCCAGAGCCTCCGGGTCTGGGCGGCGCTTCGGCTCCTCCCGAGCCGCCTGCTA
  GCCCCGCGCCGCACTCCATCCCCACAGGCTGGGGACGGGCCCGGTGCGGCTGTGTGGGTT
  CGGGAGCGGAGTTGCAGAATCCAAGGACCCATTTTGTTCTTTCTCCGCACTGCTTTATGG
  GAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATT
  CAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGA
  AAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGT
  TCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCT
  CTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTGGAGA
  ATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCT
  GCTGTGAGTTCTTAAAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTANGGGATTTT
  GCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCAT
  TTCCTGGAAGTGTACAGATGGGAGAGTTCATTCTTCTGATCAGGGAGAGGGTGGAANAGC
  TATCAGTGCGACGAATTAGGTGGATTCTGAGAGCCAGCTTGGGAGCTGTCTCAACTGGGT
  GAGCATGCCAGAAGAGCGGGAAGAATCTTTGCTACTGCTCGTATGGGGCGGATGCCCCTA
  CTAACCCAGTTTNAAGATGNAATGATGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021633 unedited
  GGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGACCGTTGATTTT
  TAATATTTTCTTAAAAAAATACAAAGGAAATTAACTCTGTAGGTCAATACAACTCAGGGA
  AAGAGGGAAAAATGGAATTTCAGAGCAAAGGTTGTTTAGGTTATCACATTCCCACACTCC
  TAATACCCACAAAACAAGAATTTCACTCCATGACACAGAGGAACATTGAATGGTAGCTCA
  GAAATGTTGATAGCTGAGGTACTGAAACTAACAAAAGGATTTTGGTTGTCCTTGATTATT
  CTGTCCTGTGATGAATAAAATCTACACTAAAGGACAGGTAAGGAAAACTTATAGCAGAAA
  AAAGACTAGATGTACCAAACACAGCAGTACAAACCACTCCTTGGCAGACATGTGCTTCTA
  AAAGAATGGGGCAGTAATCAGGTAGCTGAACTACTAGGCTACTGTCACTCCCAGCCCATC
  CCCAAATAAATAGTGTGGAAGTGTAATAGTGTAGTAGTATTTGATCCAACAAAGAAAGGC
  TTTCACCCCCATTCAAGGGCAAACATTGCCATGGCTAGATGAGCCCTGGCAGGTAATAAG
  ATGACTGTAAAATCACAGGTTCTTCTAGTTTATCCCCAGTTATACTGAACTCCCACATTG
  ATGTCATCTTCTCTCATCCCAACATTTGGTCACTCTTTTATGCTGTCTNCCGTGCGAAAG
  ACCTCGGGGGAAATAAAAAAACCTTCAAATAATATACCCTTCTCAGGCTTCCAGTTATGT
  AAACTTCAGTGCTGGTCTAGGTCACTCCCAATCAATTCAGTTAATTCACTTAATTCTACT
  TCAATCTGAAAACAAATCCGACCTCCAAAGAAGGGTCAGAGAGGCTTTTTTCCCTGGCCC
  CAATTTTGGGGGGCTCTCCTTTTGGTTCCCCTAAGGGAACTGTTGCCCTCCTCCTGTTAT
  AAAAAATGTTGTTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021633
• ORF Size: 1707 bp
• Insert Size: 3380
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_021633.2, NP_067646.1
• RefSeq Size: 3338
• RefSeq ORF: 1707
• Locus ID: 59349
• Domains: BTB, Kelch
• Gene Summary: This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (2) uses an alternate 5' terminal exon, resulting in a distinct 5' UTR and an in-frame downstream translational start site, compared to variant 1. This results in isoform 2, which has a shorter N-terminus compared to isoform 1.