POLR2L (NM_021128) Human Untagged Clone

CAT#: SC112974

POLR2L (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa (POLR2L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2L
• Synonyms: hRPB7.6; RBP10; RPABC5; RPB7.6; RPB10; RPB10beta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021128, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCATCCCTGTACGCTGCTTCACTTGTGGCAAGATCGTCGGCAACAAGTGGGAGGCTTACCTGGGGC
  TGCTGCAGGCCGAGTACACCGAGGGGGATGCGCTGGATGCCCTGGGCCTGAAGCGCTACTGCTGCCGCCG
  GATGCTGCTGGCCCACGTGGACCTGATCGAGAAGCTGCTCAATTATGCACCCCTGGAGAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021128 unedited
  CCTTTTCGGATTNTGTAAACGATTCACTATAGGCGGCCGCGCAATCGGCACGAGGCGCCG
  CCGCCATGATCATCCCTGTACGCTGCTTCACTTGTGGCAAGATCGTCGGCAACAAGTGGG
  AGGCTTACCTGGGGCTGCTGCAGGCCGAGTACACCGAGGGGGACGCGCTGGATGCCCTGG
  GCCTGAAGCGCTACTGCTGCCGCCGGATGCTGCTGGCCCACGTGGACCTGATCGAGAAGC
  TGCTCAATTATGCACCCCTGGAGAAGTGACCACGCTGGAACCCACCCACCCGCTGTGCTG
  ACCATGGGCCCTGAGCGTCCTACCCCGAATTCACGAGGCTGAGGCATCCGGGAGCTGGCG
  TAATGCCTGGCCGCAGTGTGTGTGTATCCCATACCCCACTCTGGAAGGAACCATCCAGTA
  AAGGTCTTTCAGAACCAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
  GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC
  TCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGCCCTAATAAAATTAAG
  CTGCATCATTTTGTCTGACTAGGCGCCCTTCTATAATATTATGGGGCGGAGGGGGGTGGC
  TATGGACCCATGGGGCCCAGTTGCCAAACACACACCTGTAAGGCCCCGCCGGGGCCCAAC
  TGCCGAAACCAGCCTGGACCGGCCCGGGCCACAAATCTTCGGCTCACTTGGCCATCTTTC
  CCCTTCCTGGGTTCCAACCGAATCTCCCCGCCCCCAGCCTTCCCGAGGTGGTCGGGGAAT
  ACCAGCAACTGCCTTGACCCACCCTCAACATCACCTCCTGCTTCTTTGGCCAAACCACCC
  GGCTTCCCCACCCTTGCCACCCCCGCCCCACCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021128 unedited
  CAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTGGGTCTGAAAGACTTTACTGGAT
  GGTTCCTTCCAGAGTGGGGTATGGGATACACACACACTGCGGCCAGGCATTACGCCAGCT
  CCCGGATGCCTCAGCCTCGTGAATTCGGGGTAGGACGCTCAGGGCCCATGGTCAGCACAG
  CGGGTGGGTGGGTTCCAGCGTGGTCACTTCTCCAGGGGTGCATAATTGAGCAGCTTCTCG
  ATCAGGTCCACGTGGGCCAGCAGCATCCGGCGGCAGCAGTAGCGCTTCAGGCCCAGGGCA
  TCCAGCGCGTCCCCCTCGGTGTACTCGGCCTGCAGCAGCCCCAGGTAAGCCTCCCACTTG
  TTGCCGACGATCTTGCCACAAGTGAAGCAGCGTACAGGGATGATCATGGCGGCGGCGCCT
  CGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTA
  AACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCAACG
  GGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGGTGCAAAACAAACTCC
  CATTGACGTCAATGGGGTGGAGACTTGGGAAATCCCGTGAGTCAAACCGCTATCCCGCCC
  ATTGGTGTACTGNCAAANCCGCATCACATGGTAATAGCGATGACTAATACGTANATGTAC
  TGCCAAGTANGAAAGTCCCGTAAGTCATGTACTGGGCATAATGCCCAGCGGGCCATTTAC
  CGGTATTGACGTCAATAGGGGGCCGAACTTTGCATATGAATCACTTGATGTACTCGCCAG
  TGGGCAGTTTACCGCAANTCCTCCACCCATTGCCGTCAATGGAAAGCCCCATTGTCCGTA
  CCTATGGAACATACGGTCTTTTTGACNTCAATGGCGCGGGGTCTNTTGGNCGGCAACCCC
  CCCGGCCCTTTACCGCATTATATTAACCCGGATTCCTCTTGGGCTTGAACCAAGACCCGA
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021128
• Insert Size: 400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021128.3, NP_066951.1
• RefSeq Size: 927 bp
• RefSeq ORF: 204 bp
• Locus ID: 5441
• Cytogenetics: 11p15.5
• Domains: RNA_pol_N
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]'