Reticulon 1 (RTN1) (NM_021136) Human Untagged Clone

CAT#: SC112981

RTN1 (untagged)-Human reticulon 1 (RTN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RTN1
• Synonyms: NSP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112981 sequence for NM_021136 edited (data generated by NextGen Sequencing)
  ATGGCCGCGCCGGGGGATCCGCAGGACGAGCTGCTGCCGCTGGCCGGCCCCGGGTCCCAG
  TGGCTCAGGCACCGGGGGGAGGGGGAGAACGAAGCGGTGACGCCGAAAGGGGCCACGCCG
  GCGCCGCAGGCTGGGGAGCCCAGCCCGGGGTTGGGCGCCAGGGCCCGGGAAGCGGCGTCG
  CGGGAAGCCGGCTCGGGCCCCGCCCGGCAGTCGCCCGTTGCCATGGAAACTGCATCCACA
  GGTGTGGCAGGTGTTTCCAGTGCCATGGACCACACCTTCTCAACAACATCAAAAGATGGG
  GAAGGATCGTGTTACACATCTCTCATTTCTGACATCTGCTATCCACCTCAGGAGGATTCT
  ACATATTTTACTGGAATTCTTCAGAAGGAAAATGGCCACGTCACCATTTCAGAGAGCCCT
  GAGGAGCTGGGTACACCCGGCCCCTCCTTACCAGATGTGCCTGGGATAGAGTCTCGTGGC
  TTATTTAGTTCTGATTCTGGAATAGAGATGACTCCTGCAGAGTCCACGGAAGTGAACAAG
  ATCTTAGCAGACCCTCTGGACCAGATGAAAGCAGAGGCCTATAAATACATTGACATAACC
  AGACCCGAGGAGGTGAAGCACCAAGAACAACATCACCCCGAGCTGGAAGATAAAGACTTG
  GACTTTAAGAATAAAGACACTGACATCTCAATTAAACCTGAAGGAGTCCGTGAACCTGAC
  AAACCAGCTCCTGTGGAGGGAAAAATCATCAAGGACCATTTATTGGAAGAATCCACATTT
  GCTCCATACATAGATGATCTCTCTGAAGAACAGCGCAGGGCTCCTCAGATCACCACCCCT
  GTCAAAATCACACTGACGGAAATAGAACCTTCTGTTGAAACCACTACCCAAGAGAAGACC
  CCTGAGAAGCAAGATATATGTCTAAAGCCAAGTCCTGACACAGTCCCCACTGTCACTGTC
  TCGGAGCCTGAAGACGACAGCCCAGGATCTATCACCCCTCCATCTTCTGGAACAGAACCA
  TCTGCTGCAGAATCCCAGGGGAAAGGCAGCATCTCCGAGGATGAGCTGATCACCGCCATC
  AAAGAAGCAAAGGGATTATCGTATGAAACCGCCGAGAACCCACGGCCGGTGGGCCAGCTG
  GCCGACAGGCCCGAGGTCAAGGCCAGGTCCGGACCGCCAACCATCCCCAGCCCCCTGGAC
  CACGAGGCCAGCAGCGCGGAGTCGGGGGACTCAGAGATCGAGCTGGTGTCCGAGGACCCC
  ATGGCCGCGGAGGACGCGCTGCCCTCAGGCTATGTGAGCTTTGGCCACGTGGGCGGCCCG
  CCGCCCTCGCCCGCCTCGCCATCCATCCAGTACAGCATCCTGAGGGAGGAGCGCGAGGCC
  GAGCTGGACAGCGAGCTCATCATCGAGTCGTGCGACGCCTCCTCGGCCTCGGAGGAGAGC
  CCCAAGCGGGAGCAGGACTCACCCCCGATGAAGCCCAGCGCCCTGGATGCCATCCGGGAG
  GAGACTGGCGTCCGGGCCGAGGAGCGTGCGCCAAGCCGGCGGGGCCTGGCCGAGCCGGGT
  TCCTTCCTCGACTACCCCTCAACTGAGCCCCAGCCTGGCCCCGAGCTGCCCCCTGGAGAC
  GGAGCCCTGGAGCCTGAGACGCCCATGTTGCCACGGAAGCCTGAAGAAGACTCGAGTTCC
  AACCAAAGTCCTGCGGCCACAAAGGGCCCTGGGCCTCTAGGTCCTGGCGCCCCGCCCCCA
  CTGCTGTTTCTCAATAAGCAAAAAGCTATTGACCTGTTGTATTGGCGGGACATCAAGCAG
  ACGGGCATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCAGCGTG
  GTGAGCGTCGTGGCCTACCTGGCCCTGGCCGCACTCTCAGCCACCATCAGTTTCCGCATC
  TACAAGTCTGTTTTACAAGCAGTGCAGAAAACCGACGAAGGCCACCCTTTCAAGGCCTAC
  TTGGAGCTTGAGATCACCCTTTCTCAGGAGCAGATTCAGAAGTACACGGACTGCCTGCAG
  TTCTACGTGAACAGCACACTTAAGGAACTGAGGAGGCTCTTCCTTGTCCAGGACCTGGTG
  GATTCCTTAAAATTTGCAGTCCTGATGTGGCTCCTGACCTACGTTGGCGCTCTCTTCAAT
  GGCCTGACCCTGCTGCTCATGGCTGTGGTTTCAATGTTTACTCTACCTGTAGTGTATGTT
  AAGCACCAGGCACAGATTGACCAATATCTGGGACTTGTGAGGACTCACATAAATGCTGTT
  GTGGCAAAGATTCAGGCTAAAATCCCAGGCGCTAAGAGGCACGCTGAGTAA
  
  Clone variation with respect to NM_021136.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021136 unedited
  GCACTATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGGCGAC
  GCTGAGACACCGCAGCTTCCCTGAGCGCCGAGTCCCTCCGGGGACAGCAGCAGGGAGCGC
  CCGCGCAGCCACCGAGCCTCTGCCCAGCCAAGCCGCCGTCGCCGCGCCGGGGGACCGCCA
  GCCATGGCCGCGCCGGGGGATCCGCAGGACGAGCTGCTGCCGCTGGCCGGCCCCGGGTCC
  CAGTGGCTCAGGCACCGGGGGGAGGGGGAGAACGAAGCGGTGACGCCGAAAGGGGCCACG
  CCGGCGCCGCAGGCTGGGGAGCCCAGCCCGGGGTTGGGCGCCAGGGCCCGGGAAGCGGCG
  TCGCGGGAAGCCGGCTCGGGCCCCGCCCGGCAGTCGCCCGTTGCCATGGAAACTGCATCC
  ACAGGTGTGGCAGGTGTTTCCAGTGCCATGGACCACACCTTCTCAACAACATCAAAAGAT
  GGGGAAGGATCGTGTTACACATCTCTCATTTCTGACATCTGCTATCCACCTCAGGAGGAT
  TCTACATATTTTACTGGAATTCTTCAGAAGGAAAATGGCCACGTCACCATTTCAGAGAGC
  CCTGNAGAGCTGGGTACACCCGGCCCCTCCTTACCAGATGTGCCTGGGATAGAGTCTCGT
  GGCTTATTTAGTTCTGATTCTGGAATAGAGATGACTCCTGCCGAGTCCACGGGAGTGAAC
  AAGATCTTAGCAGACCCTCTGGACCAGATGAAAGCAGAGGCCTATAATACATTGACCTAC
  CCGACCCCAGAAGTGAAGCCCCAGAACAACTCACCCCGAGCTGAAGATAAGACTTGAACC
  TTTAGAATAAGAACTGACCTCTCAATTAACCTGAAGAGCCCGGGAACCGACAACAGCTCC
  TGTGTAGGAAAAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021136 unedited
  GCACTTCTATATCGAGTTTTTTTTTTTTTTTTTTGGATTTATTCATTTATTTAATTATTT
  TAAGTTATACAAAACTGATTACCATAAGTGCGGTCGACTGCTTTTATTTTTACGTTGAGT
  GTGTTGGAAAAATGCTAAAACATCAGTCTACAATTCTATATATTGGTATTAAAGATTAAT
  CCAACCAGCAACCCAAGGACATATAAGCGATTTCCACTATTGCATCAGAGCACTCGGCAG
  GAAAGGCCTATCCACGGGGAACATTAGAAGCTACAGAAGCATTGCAGAGAAGAGAAGAAG
  AACACCGAAGAGGGAAAGATAACTTGGGCTCTCACCATCATGCACTTTTTTTAGCAACAC
  AAAATTAAAAACCACATCTAATACACTTTTCTCTATACTTTAGTGGGTGACACAAGTGAC
  TCAAATATCCTAAGAGTACAAGGAACAGCCTAAAAACAGCTGTTTTAAGGTTTGTCTCTA
  AGATTATGGCACTGGAGGGAGGGGGGAAAGACAATCAATTTGCAGTAATGAGTAAGAAGA
  GAGCTGTTACCACTCCAGACATTCCTGTTTGTGTGCAGTCCCCGGTGGGAAATCAGTTTA
  CTCAGCGTGCCTCTTATCGCCTGAGATTTTAGCCTGAATCCTTGCCACAACAGCATTTAT
  GTGAGTCCTTACAGCTCCAGATATTGGCAATCTGTGCCTGGAGCTTACATACACTACAGG
  AGAGGAAACATTGAACCACATCCTGATCATCAGGTCAGCCATTGAAAAAGCCCCACGCAG
  GCCGAGCCCCCTCAGACCGCCAATTTTAAGAACCCCCAAGTCCCTGCAAAGAAGACCCCC
  TCAGATCCTAGAGGGCCGTCACGGAAACGGCAGGAGCCCGGCCTCTTGATTGGTCCGGAA
  AGGGGATCAAGCCCAAAAGCCTGAAGGGTGCCCCCCCGTCTTGCCTGCTGAAAACAATCG
  TAACCGACCGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021136
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021136.2, NP_066959.1
• RefSeq Size: 3327 bp
• RefSeq ORF: 2331 bp
• Locus ID: 6252
• Cytogenetics: 14q23.1
• Domains: Reticulon
• Protein Families: Transmembrane
• Gene Summary: 'This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. This gene is considered to be a specific marker for neurological diseases and cancer, and is a potential molecular target for therapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A, also known as NSP-A).