KDM6A (NM_021140) Human Untagged Clone

CAT#: SC112984

KDM6A (untagged)-Human lysine (K)-specific demethylase 6A (KDM6A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM6A
• Synonyms: bA386N14.2; KABUK2; UTX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112984 sequence for NM_021140 edited (data generated by NextGen Sequencing)
  ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGAT
  GAGGAAAAGAAAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGC
  CTGACAGCCGAGGAGAGGGAGGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTG
  AGATTTCATGAAGATGGCGCCAGGACGAAGGCCCTACTGGGCAAGGCTGTTCGCTGCTAT
  GAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGATTTCTTTTGTCAATTAGGT
  CACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCAGAGGTACTAC
  AGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC
  TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGAT
  CCCAGCTTTTGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAAC
  ACAGACTATGAGTCTAGTTTAAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGC
  ACTTTGTCCAATGCTGAAATTCAATTTCACATTGCCCACTTATATGAAACCCAGAGGAAA
  TATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGACAGAGAATCTTTCTGCACAA
  GTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGATCTCCTGGGA
  GATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT
  CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTT
  CAGGATGCCTTTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACA
  TGGTGTTCAATAGGTGTGCTATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCC
  TATATTTGTGCTGTACAATTGGACCATGGCCATGCTGCAGCCTGGATGGACCTAGGCACT
  CTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAATGCTACTTAAATGCAACTAGA
  AGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTTACAGGCTCAG
  TTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAG
  GAGGCGTGGAGCCTACCAATTCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACA
  GCACAGCAGAATACTTCTGACAATTGGAGTGGTGGACATGCTGTGTCACATCCTCCAGTA
  CAGCAACAAGCTCATTCATGGTGTTTGACACCACAGAAATTACAGCATTTGGAACAGCTC
  CGCGCAAATAGAAATAATTTAAATCCAGCACAGAAACTGATGCTGGAACAGCTGGAAAGT
  CAGTTTGTCTTAATGCAACAACACCAAATGAGACCAACAGGAGTTGCACAGGTACGATCT
  ACTGGAATTCCTAATGGGCCAACAGCTGACTCATCACTGCCTACAAACTCAGTCTCTGGC
  CAGCAGCCACAGCTTGCTCTGACCAGAGTGCCTAGCGTCTCTCAGCCTGGAGTCCGTCCT
  GCCTGCCCTGGGCAGCCTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGC
  ACATCAAGAACGCTGGGAAGTACAGACACTATTTTGATAGGCAATAATCATATAACAGGA
  AGTGGAAGTAATGGAAACGTGCCTTACCTGCAGCGAAACGCACTCACTCTACCTCATAAC
  CGCACAAACCTGACCAGCAGCGCAGAGGAGCCGTGGAAAAACCAACTATCTAACTCCACT
  CAGGGGCTTCACAAAGGTCAGAGTTCACATTCGGCAGGTCCTAATGGTGAACGACCTCTC
  TCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAGCTGGCTCTGGTATTCAGAATCAGAAC
  GGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGCTCTCAATCACCTCTCC
  TCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCT
  TCAGGAAACATATTGAAGGTGCCTGAAACAAGCAGGCACACTGGAGAGACACCTAACAGC
  ACTGCCAGTGTCGAGGGACTTCCTAATCATGTCCATCAGATGACGGCAGATGCTGTTTGC
  AGTCCTAGCCATGGAGATTCTAAGTCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTC
  TCTGCCTTGTTGATGGGAAAAGCCAATAACAATGTGGGTACTGGAACCTGTGACAAAGTC
  AATAACATCCACCCAGCTGTTCATACAAAGACTGATAACTCTGTTGCCTCTTCACCATCT
  TCAGCCATTTCAACAGCAACACCTTCTCCAAAATCCACTGAGCAGACAACCACAAACAGT
  GTTACCAGCCTTAACAGCCCTCACAGTGGGCTACACACAATTAATGGAGAAGGGATGGAA
  GAATCTCAGAGCCCCATGAAAACAGATCTGCTTCTGGTTAACCACAAACCTAGTCCACAG
  ATCATACCATCAATGTCTGTGTCCATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGC
  AGGAATCTAGGTAAAAATGGCTTATCTAACAGTAGCATTTTGTTGGATAAATGTCCACCT
  CCAAGACCACCATCTTCACCATACCCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACA
  CCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACATCAATTTTGT
  ACAAATCCGAACAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAGTTAGAC
  CTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTAGAAGTG
  AGGACACAGTTGTTGCAGCCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATC
  TGGCATTGTGAAAGTAATAGATCTCATACTACAATTGCTAAATATGCACAGTACCAGGCC
  TCCTCATTCCAGGAATCATTGAGAGAAGAAAATGAAAAAAGAAGTCATCATAAAGACCAC
  TCAGATAGTGAATCTACATCGTCAGATAATTCTGGGAGGAGGAGGAAAGGACCCTTTAAA
  ACCATAAAGTTTGGGACCAATATTGACCTATCTGATGACAAAAAGTGGAAGTTGCAGCTA
  CATGAGCTGACTAAACTTCCTGCTTTTGTGCGTGTCGTATCAGCAGGAAATCTTCTAAGC
  CATGTTGGTCATACCATATTGGGCATGAACACAGTTCAACTATACATGAAAGTTCCAGGG
  AGCAGAACACCAGGTCATCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATATTGGC
  CCAGGTGACTGTGAATGGTTTGTTGTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTC
  TGTGAAAAAAATAATTTGAATTTCCTAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTT
  TATGAAGCAAATGTTCCAGTGTATAGGTTTATTCAGCGACCTGGAGATTTGGTCTGGATA
  AATGCAGGCACTGTTCATTGGGTTCAGGCTATTGGCTGGTGCAACAACATTGCTTGGAAT
  GTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTACGAATGGAACAAA
  TTGCAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCACGAAAT
  ATCAAGGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTAAGAACTCTG
  AAGCAATGTCAGACATTGAGGGAAGCTCTCATTGCTGCAGGAAAAGAGATTATATGGCAT
  GGGCGGACAAAAGAAGAACCAGCTCATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGAT
  CTGCTTTTTGTCACTAATGAGAGTAATTCACGAAAGACCTACATAGTACATTGCCAAGAT
  TGTGCACGAAAAACAAGCGGAAACTTGGAAAACTTTGTGGTGCTAGAACAGTACAAAATG
  GAGGACCTGATGCAAGTCTATGACCAATTTACATTAGCTCCTCCATTACCATCCGCCTCA
  TCTTGA
  
  Clone variation with respect to NM_021140.2
  2177 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021140 unedited
  TCACATATTATGNATACGACTCACTATAGNNGGCGGCCGCGAAATTCGGCACGAGGGAAG
  TTTGTGTCTCCAACGAATCCCCTCAGTGCTCCCCAGCCCCGCGCGCTCCGGCCGTTCCCG
  CCGTCCCCGCCTGTGGCTGCCCCCTGCCCAACCCCGCGATGTGACCCTACAGCCGAAAGC
  CGCCGCTGCCGACCCGGGGGCTCCGCAGCCCCTGCCGCCGCCGCCGCCGCCTTCACCGCC
  GCCGCGTTGGGATTTTTCGTCGCCGCCGCCCGCGGCGGAGGAGGAGGCGGCGATAAAGTT
  GGTGTGCTGGTCCCGCGCGCAGATTGGGGGCGTCACTGCGGGCCCCGGTCCGAGGGGGGG
  TGTCGGCGTTGGAGTTGTGAATTCGCTGCGTTTCCATGAAATCCTGCGGAGTGTCGCTCG
  CTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGATGAGGAAAAGAAAATGGCGGCGGGAA
  AAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCCGANGAGAGGGANGCGC
  TCTGCGGACTGGACAGCCGCCTCTTTGGGTTCGTGAGAATTCATGAAGATGGCGCCAGNA
  CGAAGGCCCTACTGGGCAAGGCTGTTCGCTGCTATGAATCTCTAATCTTAAAGCTGAAGG
  AAAGTGGAGTCTGATTTCTTTTGTCATTANGTCACTTCAACCTCTTATTGGAAGATTATC
  CAAAAGCATTATCTGCATACCAGAAGTACTACAGTTTACAGTCTGACTACTGGAAGAATG
  CTCGCCTTTTATATGGGCNTGGGGTTGGTCTACCTCCATTATAATGCATTTCAGTGGGCA
  ATTAAAGCATTTCAGGAAGTGCTTNATGNTTGATTCCAGCTTTTTGCCGAGCCCACGGAA
  ATCAATTTACCACCTTGGCTCATGTTCCAAAGTGAACACAGACTATTGATCCTAGTTTAA
  AGCATTTTCAATACCTTTGGGTGACTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021140
• Insert Size: 5400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021140.1, NP_066963.1
• RefSeq Size: 5418 bp
• RefSeq ORF: 4206 bp
• Locus ID: 7403
• Cytogenetics: Xp11.3
• Domains: TPR, JmjC
• Gene Summary: 'This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1.