Ku80 (XRCC5) (NM_021141) Human Untagged Clone

CAT#: SC112985

XRCC5 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) (XRCC5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRCC5
• Synonyms: KARP-1; KARP1; KU80; Ku86; KUB2; NFIV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112985 sequence for NM_021141 edited (data generated by NextGen Sequencing)
  ATGGTGCGGTCGGGGAATAAGGCAGCTGTTGTGCTGTGTATGGACGTGGGCTTTACCATG
  AGTAACTCCATTCCTGGTATAGAATCCCCATTTGAACAAGCAAAGAAGGTGATAACCATG
  TTTGTACAGCGACAGGTGTTTGCTGAGAACAAGGATGAGATTGCTTTAGTCCTGTTTGGT
  ACAGATGGCACTGACAATCCCCTTTCTGGTGGGGATCAGTATCAGAACATCACAGTGCAC
  AGACATCTGATGCTACCAGATTTTGATTTGCTGGAGGACATTGAAAGCAAAATCCAACCA
  GGTTCTCAACAGGCTGACTTCCTGGATGCACTAATCGTGAGCATGGATGTGATTCAACAT
  GAAACAATAGGAAAGAAGTTTGAGAAGAGGCATATTGAAATATTCACTGACCTCAGCAGC
  CGATTCAGCAAAAGTCAGCTGGATATTATAATTCATAGCTTGAAGAAATGTGACATCTCC
  CTGCAATTCTTCTTGCCTTTCTCACTTGGCAAGGAAGATGGAAGTGGGGACAGAGGAGAT
  GGCCCCTTTCGCTTAGGTGGCCATGGGCCTTCCTTTCCACTAAAAGGAATTACCGAACAG
  CAAAAAGAAGGTCTTGAGATAGTGAAAATGGTGATGATATCTTTAGAAGGTGAAGATGGG
  TTGGATGAAATTTATTCATTCAGTGAGAGTCTGAGAAAACTGTGCGTCTTCAAGAAAATT
  GAGAGGCATTCCATTCACTGGCCCTGCCGACTGACCATTGGCTCCAATTTGTCTATAAGG
  ATTGCAGCCTATAAATCGATTCTACAGGAGAGAGTTAAAAAGACTTGGACAGTTGTGGAT
  GCAAAAACCCTAAAAAAAGAAGATATACAAAAAGAAACAGTTTATTGCTTAAATGATGAT
  GATGAAACTGAAGTTTTAAAAGAGGATATTATTCAAGGGTTCCGCTATGGAAGTGATATA
  GTTCCTTTCTCTAAAGTGGATGAGGAACAAATGAAATATAAATCGGAGGGGAAGTGCTTC
  TCTGTTTTGGGATTTTGTAAATCTTCTCAGGTTCAGAGAAGATTCTTCATGGGAAATCAA
  GTTCTAAAGGTCTTTGCAGCAAGAGATGATGAGGCAGCTGCAGTTGCACTTTCCTCCCTG
  ATTCATGCTTTGGATGACTTAGACATGGTGGCCATAGTTCGATATGCTTATGACAAAAGA
  GCTAATCCTCAAGTCGGCGTGGCTTTTCCTCATATCAAGCATAACTATGAGTGTTTAGTG
  TATGTGCAGCTGCCTTTCATGGAAGACTTGCGGCAATACATGTTTTCATCCTTGAAAAAC
  AGTAAGAAATATGCTCCCACCGAGGCACAGTTGAATGCTGTTGATGCTTTGATTGACTCC
  ATGAGCTTGGCAAAGAAAGATGAGAAGACAGACACCCTTGAAGACTTGTTTCCAACCACC
  AAAATCCCAAATCCTCGATTTCAGAGATTATTTCAGTGTCTGCTGCACAGAGCTTTACAT
  CCCCGGGAGCCTCTACCCCCAATTCAGCAGCATATTTGGAATATGCTGAATCCTCCCGCT
  GAGGTGACAACAAAAAGTCAGATTCCTCTCTCTAAAATAAAGACCCTTTTTCCTCTGATT
  GAAGCCAAGAAAAAGGATCAAGTGACTGCTCAGGAAATTTTCCAAGACAACCATGAAGAT
  GGACCTACAGCTAAAAAATTAAAGACTGAGCAAGGGGGAGCCCACTTCAGCGTCTCCAGT
  CTGGCTGAAGGCAGTGTCACCTCTGTTGGAAGTGTGAATCCTGCTGAAAACTTCCGTGTT
  CTAGTGAAACAGAAGAAGGCCAGCTTTGAGGAAGCGAGTAACCAGCTCATAAATCACATC
  GAACAGTTTTTGGATACTAATGAAACACCGTATTTTATGAAGAGCATAGACTGCATCCGA
  GCCTTCCGGGAAGAAGCCATTAAGTTTTCAGAAGAGCAGCGCTTTAACAACTTCCTGAAA
  GCCCTTCAAGAGAAAGTGGAAATTAAACAATTAAATCATTTCTGGGAAATTGTTGTCCAG
  GATGGAATTACTCTGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAGGAA
  GCCAAAAAGTTTCTGGCCCCCAAAGACAAACCAAGTGGAGACACAGCAGCTGTATTTGAA
  GAAGGTGGTGATGTGGACGATTTATTGGACATGATATAG
  
  Clone variation with respect to NM_021141.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021141 unedited
  AGTTTGGATTTTGTNATCCGACTTCATATAGGGCGGCCGCGCAATTCGCACGAGGCCTCG
  TGCCGAATTCGGCACGAGGCCGGAAGCGAGTTGCGACACGGCAGGTTCCCGCCCGGAAGA
  AGCGACCAAAGCGCCTGAGGACCGGCAACATGGTGCGGTCGGGGAATAAGGCAGCTGTTG
  TGCTGTGTATGGACGTGGGCTTTACCATGAGTAACTCCATTCCTGGTATAGAATCCCCAT
  TTGAACAAGCAAAGAAGGTGATAACCATGTTTGTACAGCGACAGGTGTTTGCTGAGAACA
  AGGATGAGATTGCTTTAGTCCTGTTTGGTACAGATGGCACTGACAATCCCCTTTCTGGTG
  GGGATCAGTATCAGAACATCACAGTGCACAGACATCTGATGCTACCAGATTTTGATTTGC
  TGGAGGACATTGAAAGCAAAATCCAACCAGGTTCTCAACAGGCTGACTTCCTGGATGCAC
  TAATCGTGAGCATGGATGTGATTCAACATGAAACAATAGGAAAGAAGTTTGAGAAGAGGC
  ATATTGAAATATTCACTGACCTCAGCAGCCGATTCAGCAAAAGTCAGCTGGATATTATAA
  TTCATAGCTTGAAGAAATGTGACATCTCCCTGCAATTCTTCTTGCCTTTCTCACTTGGCA
  AGGAAGATGGAAGTGGGGACAGAGGAGATGGCCCCTTTCGCTTAGGTGGCCATGGGCCTT
  CCTTTCCACTAAAAGGAATTACCGAACAGCANANAGAAGGTCTTGAGATAGTGAAAATGG
  TGATGATATCTTTAGAAGGTGAAGATGGGTTGGNATGAAAATTTATTCATTCAGTGAGAG
  TCTGANAAAACTGTGCGTCTTTCAGAAATTTGAGAGCATTCCATTCACTGGCCCTGCCGA
  CTGACCATTGGCTCCATTTGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021141 unedited
  NTTTAGCTTGNACGCGGCCGCATCTANGATCGGTTTTTTTTTTTTTTTTTTGAGTCATTT
  ATGCCTTTAATGTTACATGTACAAAACTGTGTATTAGTGACGACTTATGAGGGTGCTGAA
  GACAGAGGTGACTGGGTACCAAGAAGTCTGTACACCAAAGAGGAACTGGAACTCAAGGCA
  AGAATGAGGACAAGAAAAAAAGAGAATAAGGGCTTTGTTATTTTCTTATTTAGTAAAGGA
  GACACCATCCACAAAGATGAAAGCTGACTTAGGTAGAAATGGGAAAGACCGAAAAGTTAC
  CTAAGTGTTTTAATATAACGAAACTTTCTCTCTTTGAGGCTTGCAAGTAGAGAACAGTGA
  AGTGATATAATTCTAACTACTGTAGTAAATTTCTCAGTTTTTGAGTTTCTAAAGGTTCAC
  TGATATGGCAGAGATTGGGCCAAAAGGTCTTTTCTTCATGAGACCACCACCATAACTCAC
  TGCTTAAAAGGAGTATGAAGGGAAATTGTGCTCCCCTTCCCACCCCACTTAAAGAAAACC
  ACCTATATACAGAGGCAGAGTAATGTGGTAACTAGAAGGCCTCAACTAAAAAGAGAAGTG
  AGTCCGTCCTTACCCATGGTTTTCCTAACCCAGCTGCTTGTTGGGAGGAAGCAAATGCTA
  CTGCTTGCTCAACACAAGTTGTGACAGCTGTTGGAGGATCAGGCAAAGGGAGACTCTGGA
  AAGGGTTAAGAAACTCATCAATAACCATGCCATGTTTCCCATGAGACACTAATTACATCA
  CAAGGGCTGCCACTGTCACTGATATACTACGTCAAGAAAGGACTACTTTCCAGAAACTTC
  AAAGCTTGTTTGTGATTTTCAAAGATCTGGTAGACCCCGAAAAAATATACCCCACCCGGA
  AATAACATGACTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021141
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021141.2, NP_066964.1
• RefSeq Size: 3310 bp
• RefSeq ORF: 2199 bp
• Locus ID: 7520
• Cytogenetics: 2q35
• Domains: VWA, Ku_C, Ku_N, ku
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]'