FGF12 (NM_021032) Human Untagged Clone

CAT#: SC113009

FGF12 (untagged)-Human fibroblast growth factor 12 (FGF12), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGF12
• Synonyms: EIEE47; FGF12B; FHF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021032, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAACAGCGACCGAG
  TGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTGTGCGAGAGGCACGTCCTCGG
  GGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGGCCGGTGAGGCGGAGACCAGAACCCCAGCTC
  AAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTG
  ATGGGACCAAGGACGAAAACAGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGC
  CATCCAAGGAGTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
  TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACACTGT
  ACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAAATTATGAAGGGGAA
  CAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCTATTGAAGTGTGTATGTACAGAGAA
  CCATCGCTACATGAAATTGGAGAAAAACAAGGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATG
  GAGGCAAAGTTGTGAATCAAGATTCAACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021032 unedited
  NGTTCAAATTTTGGTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGA
  TTTTCCACATTTTGTCGCCACTGCCCAGACTTTGACTAACCTTGTGAGCGCCGGGTTTTC
  GATACTGCAGCCTCCTCAAATTTTAGCACTGCCTCCCCGCGACTGCCCTTTCCCTGGCCG
  CCCAGGTCCTGCCCTCGCCCCGGCGGAGCGCAAGCCGGAGGGCGCAGTAGAGGCTGGGGC
  CTGAGGCCCTCGCTGAGCAGCTATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAA
  GCGGCAGGCGAGGGAGTCCAACAGCGACCGAGTGTCGGCCTCCAAGCGCCGCTCCAGCCC
  CAGCAAAGACGGGCGCTCCCTGTGCGAGAGGCACGTCCTCGGGGTGTTCAGCAAAGTGCG
  CTTCTGCAGCGGCCGCAAGAGGCCGGTGAGGCGGAGACCAGAACCCCAGCTCAAAGGGAT
  TGTGACAAGGTTATTCAGCCAGCAGGGATACTTCCTGCAGATGCACCCAGATGGTACCAT
  TGATGGGACCAAGGACGAAAACAGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCT
  GCGTGTAGTGGCCATCCAAGGAGTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGG
  CTATCTCTACAGTTCAGATGTTTTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGA
  AAACTACTATGTGATCTATTCTTCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTG
  GTTTCTGGGACTCAATAAAGAAGGTCANATTATGAAGGGGAACAGAGTGAAGAANACCCA
  AGCCTCATCACATTNTGTACCGAAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCT
  ACATGAAATGGNAGAANAACAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021032 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATTT
  AAATGTCTTTATTTGTTACTTTCCAAATATTTTGGTTAAACAAATATCTCTTGCAAATGT
  ATCTTCAAAATCTTTGCCTACATGCATACAATTTGTTCTTCCCAACTGCTTAGGGAAATT
  CCTTCATTTCATTTTCTGCTAACCTCTAGATAACAGAAATAGGATACCTGGTGAAGGATA
  TGGTCCACTATTGAAGAATAATGATTGTGTAAAGTGAGGGAAACTATATTGACCTTGAAA
  TACGGCAACCAATGGCAAGGTTTGAGGTGAGGGCCTAAAGCTTCTGCAAAGTGTATATAT
  TTTAGGGAGTCATATTTGGAACAATACATGCTGGACAGTAGAGATATAGGAGAAGTTACA
  TGCTATGTCTTCTCATTGATAATATCCATAAATCTGCATACATTTTTAACATTCTGTTTT
  GTGTTTTTTTTTCTTTAAATTTGGATGTCTCTACACCACTCCTGATTTGTAGGACTAAAT
  AGATCTATTTATTCCAATGCAAATTGTGTAACATTTATTTCTTCCTTGATTTTTAAAAAT
  ACTTTAGTATTCTTAACTATGTATGTGCCTTCTCTTACACTGAGTTCTTTTTTGCTCCTT
  TCAGCTGCTCACACAATCCTGTCTGTTGGAGTCATAGACTGCTATTCCATATTATTTCTC
  TACACACCCTTGACATTTAGTGTATTACTGGTACCCTTCTGTGTTCTTAGAAACAATGAA
  TTGCAACTGGACTTGTACAGGATCCTACTAGAGCCACAATTACTCTGGCTTGTAAGAAAG
  TAAGTAGAATTTACAGTCATGCTCCAATTCATGCCAGAGTTAAAAACAATTAGACTGTGG
  TAAGTCAAAGCAATGCATTTGCGTGACTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021032
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021032.2, NP_066360.1
• RefSeq Size: 2817 bp
• RefSeq ORF: 732 bp
• Locus ID: 2257
• Cytogenetics: 3q28-q29
• Domains: FGF
• Protein Families: Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. [provided by RefSeq, Dec 2019]'
  Transcript Variant: This variant (1) encodes a longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.