Myosin (MYL6) (NM_021019) Human Untagged Clone

CAT#: SC113035

MYL6 (untagged)-Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1


  "NM_021019" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MYL6
Synonyms ESMLC; LC17; LC17-GI; LC17-NM; LC17A; LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_021019, the custom clone sequence may differ by one or more nucleotides


ATGTGTGACTTCACCGAAGACCAGACCGCAGAGTTCAAGGAGGCCTTCCAGCTGTTTGACCGAACAGGTG
ATGGCAAGATCCTGTACAGCCAGTGTGGGGATGTGATGAGGGCCCTGGGCCAGAACCCTACCAACGCCGA
GGTGCTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGATGAATGTGAAGGTGCTGGACTTTGAGCACTTT
CTGCCCATGCTGCAGACAGTGGCCAAGAACAAGGACCAGGGCACCTATGAGGATTATGTCGAAGGACTTC
GGGTGTTTGACAAGGAAGGAAATGGCACCGTCATGGGTGCTGAAATCCGGCATGTTCTTGTCACACTGGG
TGAGAAGATGACAGAGGAAGAAGTAGAGATGCTGGTGGCAGGGCATGAGGACAGCAATGGTTGTATCAAC
TATGAAGCGTTTGTGAGGCATATCCTGTCGGGGTGA


>OriGene 5' read for NM_021019 unedited
GGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCC
GAATTCGGCACGAGGCCGGAGAGCTGAGCAGTCAAGATGTGTGACTTCACCGAAGACCAG
ACCGCAGAGTTCAAGGAGGCCTTCCAGCTGTTTGACCGAACAGGTGATGGCAAGATCCTG
TACAGCCAGTGTGGGGATGTGATGAGGGCCCTGGGCCAGAACCCTACCAACGCCGAGGTG
CTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGATGAATGTGAAGGTGCTGGACTTTGAG
CACTTTCTGCCCATGCTGCAGACAGTGGCCAAGAACAAGGACCAGGGCACCTATGAGGAT
TATGTCGAAGGACTTCGGGTGTTTGACAAGGAAGGAAATGGCACCGTCATGGGTGCTGAA
ATCCGGCATGTTCTTGTCACACTGGGTGAGAAGATGACAGAGGAAGAAGTAGAGATGCTG
GTGGCAGGGCATGAGGACAGCAATGGTTGTATCAACTATGAAGCGTTTGTGAGGCATATC
CTGTCGGGGTGACGGGCCCATGGGGCGGAGCTCGTCCGCATGGTGCTGAATGGCTGAGGA
CCTTCCCAGTCTCCCCAGAGTCCGTGCCTTTCCCTGTGTGAATTTTGTATCTAGCCTAAA
GTTTCCCTAGGCTTTCTTGTCTCAGCAACTTTCCCATCTTGTCTCTCTTGGATGATGTTT
GCCGTCAGCATTCACCAAATAAACTTGCTCTCTGGGCCAAAANNNNNNNNNNNNNNAANN
ANNNNNAAAAAAANNNNNNAAAAAAAAANAAAAAAAANNNNAAAANNAAAAAAAAAAAAA
ANNNNNNAAAAAAAAAAAANAAAAAAANNNNNNNNNAAAAAACCCCCCCTTTTTTGGGCC
GGGGTTTAGTGTTTCTTAAAAACCGGGGGGTC
>OriGene 3' read for NM_021019 unedited
AGAACTTGNGAGGCTGTTTAAACCTGTTTTTTGTCTTTGGTTTTTTTTCTTTTCTTTATA
TAATGTTGGGTAAGTTGCTGTTTACAATAAAGCCTAGGTAAACTATAGGCTAGTTACAAA
ATTCACACAGGGTAAAGGCACGGACTCTGGGGAGACTGGGAAGGTCCTCAGCCATTCAGC
ACCATGCGGACGAGCTCCGCCCCTGGGCCCGTCACCCCGCCAGGATATGCCTCACAAACG
CTTCATAGTTGATACAACCATTGCTGTCCTCATGCCCTGCCACCAGCATCTCTACTTATT
CCTCTGTCATCTTCTCACCCAGTGTGACAAGAACATGCCGGATTTTAGCACCCATGACGG
TGCCATTTCCTTCCTTGTCAAACACCCGAAGTCCTTCGACATAATCCTCATAGGTGCCCT
GATCCTTGTTCTTGGCCACTGCTTGCAACATGGGCCGAAAGTGCTCAATGTCCAGCACCT
TCACATTTATCTAATCACTCTTGGGGTTNCCCACAACCTTGAACACCTCGCCGTTGGTAG
GGTTATTGGCCCAGGGCCCTCATCACATCCCCACACTGGCTGGCCAAGATCTTTGCATGC
ACCTTCATTCGTCAACGCCACCCTGGAAAGCCTACTTGAACTTCTGGGAAATGGGTCTCG
CGCCAACTCACCCCATCTTGACTTGGTTNAGCTCTTCCGCCTGTGTCCCATTTTCCCACT
TGGGCCTTCTGCTCGATTCTCTCCCCCCCTTTTGGACGCCTGCTTCCTTAATTCTCTCAC
GTTACTATACCAAGCAAGATCNATTTAAAATCACTAATCCNNACTNCCTAACGATCATCT
TAATCAATAGGTACGGGTTTTATAAACTTTATGTCACACACCGCCCTTGTTCGATCCCTA
AAGCACTTCCTTTCCTTCCCGNGGGTTCTATTACAGCTAATACCCCAGG
Restriction Sites NotI-NotI     
ACCN NM_021019
Insert Size 860 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021019.2, NP_066299.2
RefSeq Size 704 bp
RefSeq ORF 456 bp
Locus ID 4637
Cytogenetics 12q13.2
Domains EFh
Protein Families Druggable Genome
Protein Pathways Vascular smooth muscle contraction
Gene Summary 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1), which is the major non-muscle transcript, represents the longer transcript and encodes isoform 1.

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