FBXW7 (NM_018315) Human Untagged Clone

CAT#: SC113537

FBXW7 (untagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXW7
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018315, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGTGTCCCGAGAAGCGGTTTGATACTGAGCTGCATTTGCCTTTACTGTGGAGTTTTGTTGCCGGTTC
  TGCTCCCTAATCTTCCTTTTCTGACGTGCCTGAGCATGTCCACATTAGAATCTGTGACATACCTACCTGA
  AAAAGGTTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCACAGAATCACTGAAGGGGAAA
  AATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGATTTTTTACAAAATGAAAAGAAAGTTGGACC
  ATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCAC
  TGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA
  CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATGTTTCAGAGCT
  GGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGTGAACCAACACAAGTAAAACA
  TATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTTCATTGCTCCCTAAAGAGTTGGCACTC
  TATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTT
  TGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAA
  GAGAAGAAAAGTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA
  ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGATGATCATGTGA
  TCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTC
  AGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGA
  GACAACATCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTA
  TACACACCTTATATGGGCATACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGG
  TTCTCGAGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT
  GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGATTTTATGGTAA
  AGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCATACTAATAGAGTCTATTCATT
  ACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGTGTTTGGGATGTGGAGACA
  GGGAATTGCATTCACACGTTAACAGGGCACCAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTC
  TTGTCTCTGGGAATGCAGATTCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATT
  GCAAGGTCCCAACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC
  TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTAGTCACATTGG
  AGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACAAAGCTGGTGTGTGCAGTTGG
  GAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTGGACTTTGATGTGGACATGAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018315 unedited
  TATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCATGTATGTATGTGTGTCCCG
  AGAAGCGGTTTGATACTGAGCTGCATTTGCCTTTACTGTGGAGTTTTGTTGCCGGTTCTG
  CTCCCTAATCTTCCTTTTCTGACGTGCCTGAGCATGTCCACATTAGAATCTGTGACATAC
  CTANCCTGAAAAAGGTTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCAC
  AGAATCACTGAAGGGGAAAAATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGAT
  TTTTTACAAAATGAAAAGAAAGTTGGACCATGGTTCTGAGGTCCGCTCTTTTTCTTTGGG
  AAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCACTGGGCTTGTACCATGTTCAGC
  AACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAACAACGACGCCG
  AATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATGTTTCAGAGCTG
  GAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGTGAACCAACACA
  AGTAAAACATATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTTCATTGCT
  CCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGC
  AGCTCAGACATGTCGCTACTGGAGAAATTTGGCTGAAGACAACCTTCTCTGGAGAGAGAA
  ATGCANAGAAGAGGGGATTGATGAACCATTGCACATCAAGAGAAGAAAAAGTATAAAACC
  AGGTTTCATACACAGTCCATGGNAAAGTGCATACATCAGACAGCACAGAATTGATACTAA
  CTGGNAGCGAGGAGAACTCAAATCTCCCTAGGTGCTGAAGGNACATGATGATCATGTGAT
  CACATGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018315 unedited
  GTATGGCAACTCTCCAGGNCCAGGNANAGCACTGGGGAGGGGTCACAGGGNAGCCACCCG
  GGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTT
  TTTTTTTCTTTTTGCAGGGGGAAGGGCAGGGAGTATATCGTCTACACAACTGGACAAATT
  CATCTTTTCTGCTCTTCACTTCATGTCCACATCAAAGTCCAGCACCAGCAGCTTGGTTTC
  TTCAGTCCCATTCCGACTCCCAACTGCACACACCAGCTTTGTGTTTGAGGCTCTGATCCG
  CCACACAACTCCCCCACTCCCCCCACTCTCCAATGTGACTAGGTTTCGAATAAATTCACC
  CGTTTTCAAGTCCCATAGTTTTACAGTTCCATCATCTGAGCTGGTAATTACAAAGTTCTT
  GTTGAACTGTAAACAGGTCACAGCACTCTGATGCTTGTTGGGACCTTGCAATGTTTGTAA
  ACACTGTCCTGTTTTGATATCCCAGATTTTAACTGTAGAATCTGCATTCCCAGAGACAAG
  AATATTGTCTTTGAGTTCCATTCCACTTGTTAACGACTGGTGCCCTGTTAACGTGTGAAT
  GCAATTCCCTGTCTCCACATCCCAAACACGGATTGATGTATCAAGAGATCCACTCACCAC
  ATGGATACCATCAAACTGTAATGAATAGACTCTATTAGTATGCCCCTTGCACGTGTGTAG
  ACAGTTTTCAGTCTCTGGATCCCACACCTTTACCATAAATCATATGCTNCACTAACAACC
  CTNCTGNCATNATATNGAACACAGNGNNACTGCTGCACATGACCCATCAAAACATGTAAA
  CCCTGGCCCTGTCTTAATTCCCCAACCCCTAGATGGGCTCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018315
• ORF Size: 1884 bp
• Insert Size: 2090
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018315.3, NP_060785.2
• RefSeq Size: 3603
• RefSeq ORF: 1884
• Locus ID: 55294
• Domains: WD40, F-box
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.