DIP13B (APPL2) (NM_018171) Human Untagged Clone

CAT#: SC113670

APPL2 (untagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APPL2
• Synonyms: DIP13B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018171, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGCGTTGCAGGACAGCCCCCAGACTCGCTCTTTACTGA
  GCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATACCAACCAGCTGCTCCAGGCAATGCAGCGCGT
  CTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAA
  CAGAACTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGG
  ATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATACA
  ATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTAGCAATGAGCAT
  GACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAGGTGAAGACCGAAGTCG
  GAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCACCTCTCCTCCCTTCAGTACTACTGTGCCCTCAACGC
  GCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCCCATGGACAGATTAACTTT
  TTTAAGAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCCTCCGTTGCAGACATGGTTC
  AAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCAAGAATTACTTTCTGT
  TGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATCAACAGGAACCTCATCCAGAAG
  GCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACCTGGGAGAGGCTTTATTTCTTCA
  CCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGAGGTTTGATCCAGGACCTGGACAA
  CTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTACTGCTTCCAGATCACCACGCCCAATGGAAAA
  TCGGGAATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAGTGGATATGTGCAATAAACAACATCT
  CCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATCAAGTTGAATCAGACCGCTCTGCAAGC
  AGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATGCCCCAGCCAGAACCTGAAAAATTCA
  GAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGCAACAGCCAGTCTACCTGAAGCAGAGGAGCTGA
  TCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCTGCTACAGAATTCCTTGATCAGAACAGAGG
  GAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAATCATTTCCAGAAGCAGAAGATTCTCTTTTG
  CAGCAGATGTTTATAGTTCGGTTTTTGGGATCAATGGCAGTTAAAACAGACAGCACTACTGAAGTGATTT
  ATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATTCATAACATCTTCCGCATGACAGAATCCCATCT
  GATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCACAGACTCAAGTATCAAGGGCCAATTTTGAACTT
  ACCAGTGTCACACAATTTGCTGCTCATCAAGAAAACAAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTG
  AATCCACTGGAGAAGAATCTCTGAGTACATACATTTTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTA
  TGCTATTAATTTGGGAAAAGAAATTATTGAGGTTCAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTG
  TCCATACCACTAACCAATGACGGAAAATATGTACTGTTAAACGATCAACCAGATGACGATGATGGAAATC
  CAAACGAACATAGAGGCGCAGAATCCGAAGCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018171 unedited
  GGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTGTAAAACT
  AGTCTGTGTGCATACACATGCGTTATTTCTGCTTACCTCTCTATGTGGGAAGCTGAACAT
  TTTAGGGATTAAGAAATCTGCCTCCATTGTTGTTTAGAAGCCTCTGCTCCTTGCCTGGCA
  CTACTGGCTGTTGGGAATGTGAAGATAAATAAGAGTGACTACCCTTGACTGGTTCACAGT
  TTGTTCCAGAGACAGATAATGAGGCAACAGTTCCAGTACAGTGTGAATCCTGCTGCGATA
  GGAACACAAATGCAGCTTTGAGCTCCTGCAGCTTGTACTTCTTCAGGGGCTTATTCTCTT
  GTCCTACTCGCTCTTTACTGAGCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATA
  CCAACCAGCTGCTCCAGGCAATGCAGCGCGTCTATGGAGCCCAGAATGAGATGTGCCTGG
  CCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAACAGAACTTTGCTCTTGGCA
  AAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGGATGAGCTTA
  ATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATAC
  AATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTA
  GNCATGAGCATGACCTCTCAATGGCANAATACAGCANGCTGCCTAAGAAAAAGGAGATGA
  GAAGGTGAAGACCCGAAGTCGAAAAGAGTGGGCCGCGCCCNGNCGAAGCAGCACCTCTCC
  TCCCTTCAGTACTACTGTGCCCTCCACGCGCTGCAGTACAGAAAAGCAATGGCCATGATG
  GAGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018171 unedited
  GCTATGCACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAAAAA
  AACAAAATAATTTTTATTGCTAGGTTAATTTATTACAAAGATAATAATAGTCTGTTGAAC
  TTAGTTACAGTTAAACACACTGTACCGATTCAAAATCATTATACTAGTTTGATAGGGAGG
  AGGGATGGGGGAGCGGGCTGGGGTTCCAGGAATTGGAAGTATCAGGGTGGAAATAGGAAC
  TTTATACCAAGCCCCATGACCAGCTGTGCTTCAAGTGAGTTTTAAAATTACCTGGTCCCT
  TCCCTGAAGAGGGTCTGGCTCAAAAGAAACAGGAACTGGCAGAGCTGCACCCTGTCCACA
  GTGATCCACTACTAAAAATACTCATACCTAGGAGGACCTTAAACTTTTGAATGCTGCTTC
  AGCAAGGCTCCACTGTAAAATCAAACATTTTTTGCGGTACTTATTTTTTTATTGCTTGAT
  GCCTGGAACAGTTACCGAAACTAGACCTGCATCGCGCATCTCTATTACTGTTTGGGCCTC
  AAAGCAGTGCTCGTCACCTAACTGCACAAAGAACCGCTATTACGCCCAATTGTGACATAA
  GCTGAATTATAAATACACAATTTTCAGAACGACAGGCACTCAACGCTCACTCCTTAACCC
  CATACACTCCACCTCTTAATAACAAATATTTAGGGCTCATTAAACTTGAAACCTAGACTT
  TACGCCTTTTTTAAAAAGATACTGGCAACTGTAAAAAATGACCTTAATAGGACAACGCTT
  CTCCAAATTTTTGTATAGCGCTGAAATTCCACAAATTTACTGCACCCAACAAAAAGATTT
  CCCCAAATTCCTTAAACACTTCGTAAAGGTCGGTAACCTTGCCATCATTTTCTACTCCCC
  TTCACACCTCACATTTATGGTTAAAACCCCCTCCCCAACTATGTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018171
• ORF Size: 1995 bp
• Insert Size: 3370
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018171.2, NP_060641.2
• RefSeq Size: 3289
• RefSeq ORF: 1995
• Locus ID: 55198
• Domains: PH, PID
• Gene Summary: The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) encodes the predominant isoform (1).