Apolipoprotein H (APOH) (NM_000042) Human Untagged Clone

CAT#: SC113997

APOH (untagged)-Human apolipoprotein H (beta-2-glycoprotein I) (APOH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APOH
• Synonyms: B2G1; B2GP1; BG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113997 sequence for NM_000042 edited (data generated by NextGen Sequencing)
  ATGATTTCTCCAGTGCTCATCTTGTTCTCGAGTTTTCTCTGCCATGTTGCTATTGCAGGA
  CGGACCTGTCCCAAGCCAGATGATTTACCATTTTCCACAGTGGTCCCGTTAAAAACATTC
  TATGAGCCAGGAGAAGAGATTACGTATTCCTGCAAGCCGGGCTATGTGTCCCGAGGAGGG
  ATGAGAAAGTTTATCTGCCCTCTCACAGGACTGTGGCCCATCAACACTCTGAAATGTACA
  CCCAGAGTATGTCCTTTTGCTGGAATCTTAGAAAATGGAGCCGTACGCTATACGACTTTT
  GAATATCCCAACACGATCAGTTTTTCTTGTAACACTGGGTTTTATCTGAATGGCGCTGAT
  TCTGCCAAGTGCACTGAGGAAGGAAAATGGAGCCCGGAGCTTCCTGTCTGTGCTCCCATC
  ATCTGCCCTCCACCATCCATACCTACGTTTGCAACACTTCGTGTTTATAAGCCATCAGCT
  GGAAACAATTCCCTCTATCGGGACACAGCAGTTTTTGAATGTTTGCCACAACATGCGATG
  TTTGGAAATGATACAATTACCTGCACGACACATGGAAATTGGACTAAATTACCAGAATGC
  AGGGAAGTAAAATGCCCATTCCCATCAAGACCAGACAATGGATTTGTGAACTATCCTGCA
  AAACCAACACTTTATTACAAGGATAAAGCCACATTTGGCTGCCATGATGGATATTCTCTG
  GATGGCCCGGAAGAAATAGAATGTACCAAACTGGGAAACTGGTCTGCCATGCCAAGTTGT
  AAAGCATCTTGTAAATTACCTGTGAAAAAAGCCACTGTGGTGTACCAAGGAGAGAGAGTA
  AAGATTCAGGAAAAATTTAAGAATGGAATGCTACATGGTGATAAAGTTTCTTTCTTCTGC
  AAAAATAAGGAAAAGAAGTGTAGCTATACAGAGGATGCTCAGTGTATAGATGGCACTATC
  GAAGTCCCCAAATGCTTCAAGGAACACAGTTCTCTGGCTTTTTGGAAAACTGATGCATCC
  GATGTAAAGCCATGCTAA
  
  Clone variation with respect to NM_000042.2
  796 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000042 unedited
  CATTTATGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTCATCCACAAT
  GATTTCTCCAGTGCTCATCTTGTTCTCGAGTTTTCTCTGCCATGTTGCTATTGCAGGACG
  GACCTGTCCCAAGCCAGATGATTTACCATTTTCCACAGTGGTCCCGTTAAAAACATTCTA
  TGAGCCAGGAGAAGAGATTACGTATTCCTGCAAGCCGGGCTATGTGTCCCGAGGAGGGAT
  GAGAAAGTTTATCTGCCCTCTCACAGGACTGTGGCCCATCAACACTCTGAAATGTACACC
  CAGAGTATGTCCTTTTGCTGGAATCTTAGAAAATGGAGCCGTACGCTATACGACTTTTGA
  ATATCCCAACACGATCAGTTTTTCTTGTAACACTGGGTTTTATCTGAATGGCGCTGATTC
  TGCCAAGTGCACTGAGGAAGGAAAATGGAGCCCGGAGCTTCCTGTCTGTGCTCCCATCAT
  CTGCCCTCCACCATCCATACCTACGTTTGCAACACTTCGTGTTTATAAGCCATCAGCTGG
  AAACAATTCCCTCTATCGGGACACAGCAGTTTTTGAATGTTTGCCACAACATGCGATGTT
  TGGAAATGATACAATTACCTGCACGACACATGGAAATTGGACTAAATTACCAGAATGCAG
  GGAAGTAAAATGCCCATTCCCATCAAGACCAGACAATGGATTTGTGAACTATCCTGCANA
  ACCAACACTTTATTACAAGGATAAAGCCACATTTGGCTGCCATGATGGATATTCTCTGGA
  TGGCCCGGNNAGAATAGAATGTACCAAACTGGGAAACTGGTCTGCCATGCCAAGTNTGTA
  AGCATCTTTGTAATTACCTGTGAAAAAGCCACTGTGGTGTACCAAGGNAGAGAGAGTAAG
  ATTCAGGAAAATTTNAGATGGNATGCTACATGTNGATAAGTTNCTTTCTTCTGCAAATAN
  GGNAAAGNAGTGTAGCTTACAGAGCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000042 unedited
  NTTTTACTCTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCGG
  CAATAAATTCAGTAGCTTTATTTTTAAATTTCAATTAGGTTCCTTGGATGAACAAGAAAC
  AAGTGTGACATTTTATGTGGAATCTGAAAACCACCTTAGCATGGCTTTACATCGGATGCA
  TCAGTTTTCCAAAAAGCCAGAGAACTGTGTTCCTTGAAGCATTTGGGGACTTCGATAGTG
  CCATCTATACACTGAGCATCCTCTGTATAGCTACACTTCTTTTCCTTATTTTTGCAGAAG
  AAAGAAACTTTATCACCATGTAGCATTCCATTCTTAAATTTTTCCTGAATCTTTACTCTC
  TCTCCTTGGTACACCACAGTGGCTTTTTTCACAGGTAATTTACAAGATGCTTTACAACTT
  GGCATGGCAGACCAGTTTCCCAGTTTGGTACATTCTATTTCTTCCGGGCCATCCAGAGAA
  TATCCATCATGGCAGCCAAATGTGGCTTTATCCTTGTAATAAAGTGTTGGTTTTGCAGGA
  TAGTTCACAAATCCATTGTCTGGTCTTGATGGGAATGGGCATTTTACTTCCCTGCATTCT
  GGTAATTTAGTCCAATTTCCATGTGTCGTGCAGGTAATTGTATCATTTCCAAACATCGCA
  TGTTGTGGCAAACATTCAAAAACTGCTGTGTCCCGATAGAGGGAATTGTTTCCAGCTGAT
  GGCTTATAAACACGAAGTGTTGCAAACGTANGTATGGATGGTGGAGGGCANATGATGGGA
  GCACAGACAGGAAAGCTCCGGCTNCATTTTCCTTTCTCAGTGCACTTGGCAGAATCAGCG
  CCATTCAGATAAACCCAGTGTTACAAGAAAACTGATCGTGTGGGNAATTCAAAAGTCGTA
  TAGCGTACGGCTCCATTTTCTAAGATCCAGCAAAGGACTACTCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000042
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000042.1, NP_000033.1
• RefSeq Size: 1182 bp
• RefSeq ORF: 1038 bp
• Locus ID: 350
• Cytogenetics: 17q24.2
• Domains: CCP
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'Apolipoprotein H, also known as beta-2-glycoprotein I, is a component of circulating plasma lipoproteins. It has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, hemostasis, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome (APS). The anti-beta (2) glycoprotein I antibodies from APS patients, mediate inhibition of activated protein C which has anticoagulant properties. Because beta-2-GPI is the main autoantigen in patients with APS, the disruption of this pathway by autoantibodies may be an important mechanism for thrombosis in patients with APS.[provided by RefSeq, Dec 2019]'