NKRF (NM_017544) Human Untagged Clone

CAT#: SC114069

NKRF (untagged)-Human NFKB repressing factor (NKRF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NKRF
• Synonyms: ITBA4; NRF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017544 edited
  ATGGAAAAAATTCTCCAAATGGCTGAAGGTATTGATATTGGGGAGATGCCTTCATATGAT
  CTGGTGCTGTCCAAACCTTCCAAAGGTCAAAAACGCCACCTCTCAACATGTGATGGTCAA
  AATCCTCCTAAAAAGCAAGCCGGTTCCAAATTCCATGCGAGACCTCGTTTTGAGCCTGTA
  CATTTTGTAGCTAGTAGTTCAAAAGATGAAAGACAGGAAGATCCTTATGGCCCTCAAACA
  AAAGAGGTAAATGAACAAACACATTTTGCCAGCATGCCAAGAGACATCTACCAAGATTAT
  ACTCAAGACTCTTTCAGTATACAAGATGGGAATTCTCAGTATTGTGATTCATCAGGATTC
  ATTCTCACAAAAGACCAGCCTGTAACAGCCAACATGTATTTTGACAGTGGGAACCCTGCC
  CCAAGCACCACATCACAGCAGGCAAACTCTCAGTCAACTCCTGAGCCTTCACCATCACAG
  ACATTTCCCGAGTCTGTGGTAGCCGAGAAGCAGTATTTTATTGAAAAATTAACGGCGACA
  ATCTGGAAGAACCTTTCTAATCCAGAAATGACTTCTGGATCTGATAAAATTAATTATACA
  TATATGTTAACTCGTTGTATTCAGGCGTGTAAGACAAATCCTGAGTATATATATGCTCCT
  TTAAAGGAAATTCCTCCTGCCGACATCCCCAAAAATAAAAAACTTCTAACTGATGGCTAT
  GCTTGTGAAGTTAGATGCCAAAATATCTACTTAACTACAGGTTATGCTGGCAGCAAGAAT
  GGGTCCAGGGATCGAGCTACAGAGCTAGCTGTAAAACTCTTGCAGAAACGTATTGAAGTT
  AGAGTTGTCCGGCGGAAATTCAAGCATACATTTGGAGAGGACCTCGTGGTGTGTCAGATT
  GGCATGTCCTCCTATGAATTTCCTCCAGCTCTGAAGCCACCAGAAGACCTGGTGGTGCTG
  GGTAAAGATGCTTCCGGGCAGCCAATTTTTAATGCTTCTGCCAAACACTGGACCAATTTT
  GTCATTACAGAAAATGCAAATGATGCAATTGGTATCCTTAACAATTCTGCCTCATTCAAC
  AAGATGTCAATTGAATACAAATATGAGATGATGCCAAATCGCACATGGCGTTGTCGAGTG
  TTTTTACAAGATCACTGCTTAGCTGAAGGTTATGGAACCAAGAAAACAAGTAAACATGCA
  GCTGCCGACGAGGCTTTGAAAATTCTTCAAAAAACACAGCCCACTTATCCATCTGTCAAA
  AGTTCACAATGCCATACAGGCTCTTCACCCAGAGGATCTGGAAAGAAGAAAGATATAAAG
  GATCTTGTAGTTTATGAGAATTCTTCAAATCCCGTGTGCACGCTGAACGACACAGCTCAG
  TTTAACCGAATGACAGTTGAGTATGTCTATGAAAGGATGACAGGCCTCCGCTGGAAATGC
  AAAGTGATTCTAGAGAGTGAAGTAATTGCAGAAGCAGTTGGGGTGAAGAAAACTGTCAAA
  TATGAAGCTGCTGGGGAAGCTGTGAAAACCCTCAAAAAGACCCAGCCAACTGTCATTAAC
  AACTTGAAGAAAGGAGCTGTTGAAGATGTGATTTCAAGAAATGAAATTCAGGGCCGCTCA
  GCAGAGGAGGCTTACAAACAGCAAATCAAAGAAGATAATATTGGAAATCAGCTGCTGAGA
  AAGATGGGTTGGACTGGTGGTGGTTTAGGTAAATCTGGTGAGGGCATACGGGAGCCTATC
  TCAGTGAAAGAGCAGCATAAGCGGGAAGGGCTTGGTCTGGATGTAGAGAGGGTGAATAAA
  ATTGCCAAGAGAGATATTGAACAGATCATCAGAAACTACGCCCGCTCCGAGAGCCACACA
  GATTTGACTTTCTCTAGAGAGCTGACTAATGATGAACGGAAGCAAATACATCAGATTGCC
  CAGAAGTATGGTCTTAAGAGTAAGTCTCATGGGGTGGGCCATGATAGGTACCTAGTGGTA
  GGTAGAAAAAGACGGAAGGAAGACCTACTAGATCAGCTCAAACAGGAAGGCCAAGTGGGC
  CATTACGAGCTTGTTATGCCTCAAGCAAATTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017544 unedited
  GTTCAAATTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCACTTTGCCA
  GTAAGAGTCTATTCAAATTGGCTTCTGAGTCCATTTGACACAACACCTTTGATCTTTGAC
  AGTTTCCTTGGTTTTAGGTGCTAGATGATTTCTCAGGCTCACCTTAGACATTTCCTGCCA
  CAGACTTAGAATCAGCCATTTCTCTAAGGACCCTGATTCCATTTCATGAGAAATGATAGA
  GACCACAATCAAAACAAGTCATGAATTTATACTGATATTTTCAATTCAAATTAAAGATGA
  GGTTTTTGCTAAATTTTTTTGAGTTTATATTTGTATGTCTTATGCTGAAAAATCTTGTTT
  CCTAATTAGTAACATAATTATTCATTTGATGGGTAAATATTTTAGGGCCGATTCTTTGGT
  TTTATAGCCAAGATACCCTGTTGATAAAGTCTTGTGGGAGCAATTATAAGACTGGCTTAT
  TTTGAAGCTTTTTAAAAAAAGACATCCTTACCTGTTTTAACTGTAGATTATATTAACTTA
  AATAGGTACAGCCCACGCTTGATGGAAAAAATTCTCCAAATGGCTGAAGGTATTGATATT
  GGGGAGATGCCTTCATATGATCTGGTGCTGTCCAAACCTTCCAAAGGTCAAAAACGCCAC
  CTCTCAACATGTGATGGTCAAAATCCTCCTAAAAAGCAAGCCGGTTCCAAATTCCATGCG
  AGACCTCGTTTTGAGCCTGTACATTTTGTAGCTAGTAGTTCAAAAGATGAAAGACAGGAA
  GATCCTTATGGCCCTCAACAAAAGAGTAAATGAACANCACATTNTGCCAGCATGCCAAGA
  GACTCTACCAAGATATACTCAGACTCTNTCATATACAGATGGGAATCTCAGTATGTGATC
  ATCAGGATCATCTCACAAAGACAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017544 unedited
  GTACCGCGGCACGCAATCTAGTGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT
  GATCAACTTTGGCTCCTTTTTATTCAGGAAGCATACACTAATTCTTTTTATACATTTTTT
  TTTTTTACATTCCAAAAATAAGCGAAAATAGCAGTTTTAAATATGTTACAAAGTTGGCTT
  TTCAGGCTTAAGCTTTTTGAGAGAGGGACCAGCCAACTGACCTTTTCATCAAAGCACCAA
  TGTATCTTCAGTCTTTTACCAAGCTCTACCACAGCTGGGGTTGCCTTGGATGTGCTGCAC
  ATCCAAACCGGGTGAAAGCTATAATGGAGAGTTGCTGGGAGTCTCTCTGCAAGGCACCAA
  GAACAAACAGTTCAAGCTTCCTTTCTTCAAATTGAACCAAAGTTTTTGAAACTTTCAACT
  GCTCTCTCAAGTAGGGCTCTCTCAGCGTGAAGACTAGAATTTGTTAAAACCCATATATCA
  CAAGATACAGTAAATCACTTCCAATAGTCTGGAAATCACATGGTAGAAGTTATAAATAAA
  TGAACACTAAAATTACTTTAAGAAAGTTTTACATCATTTAGTTGGTAAGGACATTTACGT
  GTACAGCACAATTTATTGTCAAACATTCTATAACTGGCTTCCACAAAGATGAAAATAGAT
  TAATACAAAATGATCTGACCTTTTCAGTAAATCTGGAATTAAGACTGGATTTTTATTGGA
  GNCAATATCAACTTTACATATTGTTGCAGATTGGCTGGGCTATGCCCCTTTAAATGANTA
  TTCTCAGGGTAAATTCCATTGGGATATNGGTCCTGGCTTGGCAAACTACTAAATCTGAGG
  GACCAGCATTTCAAGACCCAACAGTACCTGACATTTCATCGCTGGATAAGCTATAACAGG
  GACAGATTCTTTAGTCCATTTCTATAACCCCTTGGGATGAACAGGGGGATTTTATGATTC
  CTTGACGGAATGGAGACTCCTTATTAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017544
• ORF Size: 1167 bp
• Insert Size: 3100
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_017544.1, NP_060014.1
• RefSeq Size: 3736
• RefSeq ORF: 1167
• Locus ID: 55922
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2).