VAMP1 (NM_016830) Human Untagged Clone

CAT#: SC114137

VAMP1 (untagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAMP1
• Synonyms: CMS25; SPAX1; SYB1; VAMP-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016830, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGTCCCCCTGGCC
  CTCCTCCTAACATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACAT
  CATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGACCGAGCTGAT
  GCCTTGCAGGCAGGAGCATCACAATTTGAGAGCAGTGCTGCCAAGCTAAAGAGGAAGTATTGGTGGAAAA
  ACTGCAAGATGATGATCATGCTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGGCGGGACTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016830 unedited
  CATATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCCGTCCACTTC
  AGCCGCAGCGTCCCTCGCCGGGTGTCTCGCCGCAGCCTCCGGAGAGGAACAGACCCTCAC
  TCTCTCTGTCAGAAAAATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGAC
  TGCCCCAGGTGGGGGTCCCCCTGGCCCTCCTCCTAACATGACCAGTAACAGACGACTACA
  GCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACATCATACGTGTGAACGTGGACAAGGT
  CCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGACCGAGCTGATGCCTTGCAGGCAGG
  AGCATCACAATTTGAGAGCAGTGCTGCAAAGCTAAAGAGGAAGTATTGGTGGAAAAACTG
  CAAGATGATGATCATGCTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGTAA
  GTATCGCTGAAGTTGCTGGTGGGGTGAAGAGGTGGGAAGGTCCTGGAGTCTTCTCCCTGC
  CTGCCTGCCTGCCTGGGGAGTGGGGCTGCTCTGACTGAGGTATGGAGACCGCTGCTATGG
  GATCGTCANCCTGGTCCGGGACGGGNGGAAGGGCAGAGACAAGGGATTTCTTTGATGGAC
  AAGCCTTCCCCCTGCAAAGCATTGCNAGGAGATGGCGCCTCCTGTTCTGAGGAAGTGGGG
  CNTATGCTGTGTGTCACTGGGCTTGGGNTGGNANGGAGAACCATGGGGGATGGGGGGCAC
  TTAACAATGGAAAGACCCCTGATGTCTGGGTACCTNGGNTGTNAATTCGCCCTCNTGGTT
  TNCTNCCTGGCTCTTTCTCCTTTTTCGTCTGGGACTTNCTGATTNCCTGNNGNCCAGNNC
  TACTTTTTACCTGGGAGAATGGTACACCCCCTTNNCCTGTTTGTCCATTGCCATCACCAA
  TTCATGGTCCTCTGGCCTTCNGTGTTTGCTCTTAACCCACTTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016830 unedited
  NNCCTTTTGACTTGNACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTAAAGGGGAGGAAATTTGAATTTTTTGCCTTTGTTCAAAATAC
  ATGACATTGGTAAATATGCCACATGCCTTTGGGGGAAGTACAACTGTTGTTATTACTCTA
  TACAAGTATGAAATCAGGGTTAGGAAAAAAAGACAAAGAGGTGATGACAGACACACAGGG
  GAAACCCCACATCGTCTCATGGCAAACCGAAAAACGGGATGTGGGAAGCTCAGCTTCATT
  TGACTGCAAAGTCCCAGGGTTTTGTTGCACATTTGCTCATGCACATGGGTGGGGGGAGGA
  AAGGGGGATAGCAAAGACACACAGAAGAGGGTACAGGGTGGGTGAAAAAGAAAGTAAAAG
  GGCTAATACCCCCAAAGAACAAGGCCAACTACACCTGGTGAGCCTCAGAGGGACAGAAAC
  CCAGGAATGATTCCTGTGATAGGGCTGGGAGAGCCAAAAGGACGGATTCGCTCCAGGCTT
  GGGACACATCGAGGACAGTAGGTGGTTCTTCTCCAGCGGTGACCCCCTGCATTAGGCAAG
  GAGGAGCCCAGAGGAGAGTGGAGACCTTCGAGGGGGGCCGTTGGGAGGGTACTGACTGCT
  TTCTTCCAGCTCTTCAATCCCGCCCTTGGGCAGAACCATAGGGAATGTGGGAAACAAGGG
  CGAAGGGAAAGGAAAGATGGTTTCAGAGCCATGCAATGCTGCTGGGTGGAAAGTGGGCAT
  ACAGACCCTGACCAGGATGGCCTCAGCCTTTTCCTGTTCGTGGAACCGAGGGAAAAAGGA
  AAAAAGGCCCAGGGCGATTTTCCCGTTATGTCACAGCTTGCCTCCTCCCTTGGCCCGGGG
  CTATCCCAAGTATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016830
• Insert Size: 4360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016830.2, NP_058439.1
• RefSeq Size: 1104 bp
• RefSeq ORF: 351 bp
• Locus ID: 6843
• Cytogenetics: 12p13.31
• Domains: synaptobrevin
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (3), also known as VAMP-1B, contains an alternate 3' terminal exon, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1. Isoforms 3 and 4 are the same length but differ in their C-termini.