NT5C3 (NT5C3A) (NM_001002010) Human Untagged Clone

CAT#: SC114247

NT5C3 (untagged)-Human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NT5C3A
• Synonyms: cN-III; hUMP1; NT5C3; P5'N-1; P5N-1; p36; PN-I; POMP; PSN1; UMPH; UMPH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114247 sequence for NM_001002010 edited (data generated by NextGen Sequencing)
  ATGAGGGCCCCGTCCATGGACCGCGCGGCCGTGGCGAGGGTGGGCGCGGTAGCGAGCGCC
  AGCGTGTGCGCCCTGGTGGCGGGGGTGGTGCTGGCTCAGTACATATTCACCTTGAAGAGG
  AAGACGGGGCGGAAGACCAAGATCATCGAGATGATGCCAGAATTCCAGAAAAGTTCAGTT
  CGAATCAAGAACCCTACAAGAGTAGAAGAAATTATCTGTGGTCTTATCAAAGGAGGAGCT
  GCCAAACTTCAGATAATAACGGACTTTGATATGACACTCAGTAGATTTTCATATAAAGGG
  AAAAGATGCCCAACATGTCATAATATCATTGACAACTGTAAGCTGGTTACAGATGAATGT
  AGAAAAAAGTTATTGCAACTAAAGGAAAAATATTACGCTATTGAAGTTGATCCTGTTCTT
  ACTGTAGAAGAGAAGTACCCTTATATGGTGGAATGGTATACTAAATCACATGGTTTGCTT
  GTTCAGCAAGCTTTACCAAAAGCTAAACTTAAAGAAATTGTGGCAGAATCTGACGTTATG
  CTCAAAGAAGGATATGAGAATTTCTTTGATAAGCTCCAACAACATAGCATCCCCGTGTTC
  ATATTTTCGGCTGGAATCGGCGATGTACTAGAGGAAGTTATTCGTCAAGCTGGTGTTTAT
  CATCCCAATGTCAAAGTTGTGTCCAATTTTATGGATTTTGATGAAACTGGGGTGCTCAAA
  GGATTTAAAGGAGAACTAATTCATGTATTTAACAAACATGATGGTGCCTTGAGGAATACA
  GAATATTTCAATCAACTAAAAGACAATAGTAACATAATTCTTCTGGGAGACTCCCAAGGA
  GACTTAAGAATGGCAGATGGAGTGGCCAATGTTGAGCACATTCTGAAAATTGGATATCTA
  AATGATAGAGTGGATGAGCTTTTAGAAAAGTACATGGACTCTTATGATATTGTTTTAGTA
  CAAGATGAATCATTAGAAGTAGCCAACTCTATTTTACAGAAGATTCTATAA
  
  Clone variation with respect to NM_001002010.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001002010 unedited
  TATAGGGCGGCCGCGAATTCGCACGAGGCTGGGATCCCGCGCTTCCGAGACTCGCAGTCT
  ACGCGAGCTGCCTGTTTTTTTCCTGCTTGGACGCGCATGAGGGCCCCGTCCATGGACCGC
  GCGGCCGTGGCGAGGGTGGGCGCGGTAGCGAGCGCCAGCGTGTGCGCCCTGGTGGCGGGG
  GTGGTGCTGGCTCAGTACATATTCACCTTGAAGAGGAAGACGGGGCGGAAGACCAAGATC
  ATCGAGATGATGCCAGAATTCCAGAAAAGTTCAGTTCGAATCAAGAACCCTACAAGAGTA
  GAAGAAATTATCTGTGGTCTTATCAAAGGAGGAGCTGCCAAACTTCAGATAATAACGGAC
  TTTGATATGACACTCAGTAGATTTTCATATAAAGGGAAAAGATGCCCAACATGTCATAAT
  ATCATTGACAACTGTAAGCTGGTTACAGATGAATGTAGAAAAAAGTTATTGCAACTAAAG
  GAAAAATATTACGCTATTGAAGTTNGATCCTGTTCTTACTGTAGAAGAGAAGTACCCTTA
  TATGGTGGAATGGTATACTAAATCACATGGTTTTGCTTGTTCAGCAAGCTTTACCAAAAG
  CTAAACTTAAAGAAATTGTGGCAGATNNCTGACGTTATGCTCANAGAAGGATATGAAGAA
  ATTTCTTTGATAAGCTCCAACACATAGCATCCCCCGTGTTCATATTTTTCGGCTGGATCG
  GGCGATGACTTNAAAGGAAGTTATTCGTCAGCTGGGTGTTTATATCCATGTCAAAAATTG
  TGTCATTTATAAGGGATTTGATAAACCGGGGGTGCTCAAAGATTTAAAGGAGACTAATTC
  ATGTATTTACAACCTTGAGGGGCCTTTGAGGATACAGAATATTTAATCACTAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001002010 unedited
  NNTTTTTACTCTGGACNCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTA
  AAAGGTTTTGCATTTCATATTTATTATCAGTGCTTCAATATAGAATGTTTTGTAATGATT
  AGCAACATTGTAAACACCACCAGGTTTTTCCAACACTAACTGCTAGTCTTTTCCAGTAAA
  TATTTTTTCCCCAGACAAAATAACCAAACCTAACACAATTTCAAGAGATGGTGATACCAT
  CAGCATAATAACCCAAATTACAAAAATGGCAATACTTAAAATCATGCATATTATTTCACC
  ATTTCATAAAACTTATTTTAAAATTTCTACAAACTTTCCCAGTGTAAAAGACTCCTCAAA
  AGCTGCATGATAACATTAAACTACAAAATTCACTTTGGAAAAGTAGGGAGTTATTTTTCT
  AATTTTAGCTTTTTTTTTTTTAAATGGGTTAAAAGATACGGTGAGGAGTGTGTTGAGAGA
  GGTGGAGAAAAGGAGCTTCCAGTCAATGCATTCACCATATCTGAAAATACTTCAGTTATA
  CAAAGGGAACACTTCGAGAGTAAGGATATATTATAAATAAGTCTCTCAGCAAGATGAACG
  GATGAACAGTTCAATTGCACCCACAGGAGAGAGGTCTTCTTGGAGAATGCTTGTTTATAG
  AATCTTCTGTAAAATAGAGTTGGCTACTTCTAATGATTCATCTTGTACTAAAACAATATC
  ATAAGAGTCCATGTACTTTTCTAAAAGCTCATCCACTCTATCATTTAGATATCCAATTTT
  CAGAATGTGCTCAACATTGGCCACTNCATCTGCCATTCTTAAAGTCTCTTGGGAGTCTNC
  CAGAGAATATGNTACTATTGGCTTTTAGNNTGATGAAAAATCTGTATCCTCAGGCACCAT
  CATGNNTGTAATACAGAAATAGNTCTCTTTAAATCTTGAGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001002010
• ORF Size: 1011 bp
• Insert Size: 1840
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001002010.1, NP_001002010.1
• RefSeq Size: 1688
• RefSeq ORF: 1011
• Locus ID: 51251
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1). CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is more supported by conservation data.