Leucyl tRNA synthetase (LARS) (NM_016460) Human Untagged Clone

CAT#: SC114279

(untagged)-Human leucyl-tRNA synthetase (LARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LARS
• Synonyms: hr025Cl; HSPC192; ILFS1; LARS1; LEURS; LEUS; LFIS; LRS; PIG44; RNTLS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016460, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAAAGAAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAAT
  GGGATACTGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGGGCAAGTATTT
  TGTAACCTTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACACGTTTTCTTTATCCAAATGT
  GAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAGATGTTGTCTGTTTCCCTTTGGCCTGCACTGTACTG
  GAATGCCTATTAAGGCATGTGCTGATAAGTTGAAAAGAGAAATAGAGCTGTATGGTTGCCCCCCTGATTT
  TCCAGATGAAGAAGAGGAAGAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCT
  AAAGGAAAAAAGAGTAAAGCTGCTGTAAAGCTGGATCTTCTAAATACCAGTGGGGCATTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016460 unedited
  NCGCGATAAATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTTA
  GTTTCGTGGGAGGCCTGGCATCCCCGAGAGGGAGGGGAAAGGTAACCACTCCTTTGTGGA
  GGTCGCCAGGGTCATTGTCGTGGATTTGCACAGTCGGCTGGGCGGTGCAATGGCGGAAAG
  AAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAATG
  GGATACTNGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGG
  GCAAGTATTTTGTAACCTTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACA
  CGTTTTCTTTATCCAAATGTGAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAAATGTT
  GTCTGTTTCCCTTTGGCCTGCACTGTACTGGAATGCCTATTAAGGCATGTGCTGATAAGT
  TGAAAAGAGAAATAGAGCTGTAATGGTTGCCCCCCTGATTTTCCAGATGAAGAAGAGGAA
  GAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCTAAAGGAAAA
  AAGAGTAAAGCTGCTGCTAAAGCTGGATCTTCTAATACCAGTGGGGCATTATGAAATCCT
  TGGCCCTGTCTGATGAAAGAGATAGTAAATTTTCTGGAGCAGACATTGGGCTTNGATATT
  TCCNGCACTGGCTATNCNGATTAAAAGAAAGGGGNTNATAAAGAAAATGGGGGGGNNNTT
  CTTATACCCCCGGGAGATATCCTACAATGAATCCTTGGGCAAAGGGCATTTTTTACATTT
  AAGAGAAGAACAAATTAATTGGGGGAAAGGGGTTTCAATTACTCTCCGAAGATGACGCCC
  TGCCTGGATATGATAATGGAGAAGGAGGGGGGGGGGCCCACAAGGATTCACTTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016460
• ORF Size: 483 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016460.1, NP_057544.1
• RefSeq Size: 722
• RefSeq ORF: 483
• Locus ID: 51520
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.