PIAS1 (NM_016166) Human Untagged Clone

CAT#: SC114401

PIAS1 (untagged)-Human protein inhibitor of activated STAT, 1 (PIAS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIAS1
• Synonyms: DDXBP1; GBP; GU/RH-II; ZMIZ3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114401 sequence for NM_016166 edited (data generated by NextGen Sequencing)
  ATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAA
  GTACTGTTGGGCTACGCCGGGAGAAACAAGCACGGACGCAAACACGAACTTCTCACAAAA
  GCCCTGCATTTGCTAAAGGCTGGCTGTAGTCCTGCTGTGCAAATGAAAATTAAGGAACTC
  TATAGGCGGCGGTTCCCACAGAAAATCATGACGCCTGCAGACTTGTCCATCCCCAACGTA
  CATTCAAGTCCTATGCCAGCAACTTTGTCTCCATCTACCATTCCACAACTCACTTACGAT
  GGTCACCCTGCATCATCACCATTACTCCCTGTTTCTCTTCTGGGACCTAAACATGAACTG
  GAACTCCCACATCTTACATCAGCTCTTCACCCAGTCCATCCGGATATAAAACTTCAAAAA
  TTACCATTTTATGATTTACTGGATGAACTGATAAAACCCACCAGTCTAGCATCAGACAAC
  AGTCAGCGCTTTCGAGAAACCTGTTTTGCATTTGCCTTGACACCACAACAAGTGCAGCAA
  ATCAGTAGTTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTACAGGTCCAGTTA
  AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAATCTTTGT
  GTGAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACCTTCCACCTACAAAAAATGGC
  GTGGAACCAAAGCGACCCAGCCGACCAATTAATATCACCTCACTTGTCCGACTGTCCACA
  ACAGTACCAAACACGATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAAACTATTCCATG
  GCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG
  GGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAAGTTGACTGCGGATCCA
  GACAGTGAAATAGCTACAACCAGCCTAAGGGTTTCTCTACTATGTCCACTTGGTAAAATG
  CGGCTGACAATTCCGTGTCGGGCCCTTACATGTTCTCATCTACAATGTTTTGACGCAACT
  CTTTACATTCAGATGAATGAGAAAAAACCAACCTGGGTTTGTCCTGTCTGTGATAAGAAG
  GCTCCATATGAACACCTTATTATTGATGGCTTGTTTATGGAAATCCTAAAGTACTGTACA
  GACTGTGATGAAATACAATTTAAGGAGGATGGCACTTGGGCACCGATGAGATCAAAAAAG
  GAAGTACAGGAAGTTTCTGCCTCTTACAATGGAGTCGATGGATGCTTGAGCTCCACATTG
  GAGCATCAGGTAGCGTCTCACCACCAGTCCTCAAATAAAAACAAGAAAGTAGAAGTGATT
  GACCTAACCATAGACAGTTCATCTGATGAAGAGGAAGAAGAGCCATCTGCCAAGAGGACC
  TGTCCTTCCCTATCTCCCACATCACCACTAAATAATAAAGGCATTTTAAGTCTTCCACAT
  CAAGCATCTCCAGTATCCCGCACCCCAAGCCTTCCTGCTGTAGACACAAGCTACATTAAT
  ACCTCCCTCATCCAAGACTATAGGCATCCTTTCCACATGACACCCATGCCTTACGACTTA
  CAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCTTG
  CTTGCCGCTGCAGCAGCAGCAGTTTCAGATGATCAAGACCTCCTACACTCGTCTCGGTTT
  TTCCCGTATACCTCCTCACAGATGTTTCTTGATCAGTTAAGTGCAGGAGGCAGTACTTCT
  CTGCCAACCACCAATGGAAGCAGTAGTGGCAGTAACAGCAGCCTGGTTTCTTCCAACAGC
  CTAAGGGAAAGCCATAGCCACACCGTCACAAACAGGAGCAGCACGGACACGGCATCCATC
  TTTGGCATCATACCAGACATTATTTCATTGGACTGA
  
  Clone variation with respect to NM_016166.1
  318 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016166 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAAGATGGCGGACAGTG
  CGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAAGTACTGTTGGGCT
  ACGCCGGGAGAAACAAGCACGGACGCAAACACGAACTTCTCACAAAAGCCCTGCATTTGC
  TAAAGGCTGGCTGTAGTCCTGCTGTGCAAATGAAAATTAAGGAACTCTATAGGCGGCGGT
  TCCCACAGAAAATCATGACGCCTGCAGACTTGTCCATCCCCAACGTACATTCAAGTCCTA
  TGCCAGCAACTTTGTCTCCATCTACCATTCCACAACTCACTTACGATGGTCACCCTGCAT
  CATCACCATTACTCCCTGTTTCTCTTCTGGGACCTAAACATGAACTGGAACTCCCACATC
  TTACATCAGCTCTTCACCCAGTCCATCCGGATATAAAACTTCAAAAATTACCATTTTATG
  ATTTACTGGATGAACTGATAAAACCCACCAGTCTAGCATCAGACAACAGTCAGCGCTTTC
  GAGAAACCTGTTTTGCATTTGCCTTGACACCACAACAAGTGCAGCAAATCAGTAGTTCCA
  TGGATATTTCTGGGACCAAATGTGACTTCACAGTACAGGTCCAGTTAAGGGTTTTGTTTA
  TCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAATCTTTGTGTGAAAGTGAAT
  ACAAAACCTTGCAGCCTTTNCAGGTTACCTTCCACCTACAAAAAAATGGCGTGGAACCAA
  AGCGACCCAGCCGACCAATTAATATCACCTCACTTGTNCGACTGTCCACAACAGTACCCA
  CACGAATTGTTGTTNCNTGGACTGCAGAAATGGAAGAAACTATTCATGGCAGTATATCTT
  GTAAAACAGNTGTCCTCAACAGTTCTCTTTCAGAGGTACGGCAAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016166 unedited
  CGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCTTTCCTTTTTTTTTTTAAA
  CAAGTGTTTTATCACACAGGCAGTCTTAAATTTATATACAAAAGTAAAACTGAAAATATA
  GTTTTGTTCTCTGTACATTTCTTTTAATTTTTTCCCTAAAAAAAGGAAAAAAAACACGCT
  TGTATACTTTTCTAAACAGAGTAAGGTAAAACAGAGCACAAAGTTCTCTTCTTTCTGCCA
  AGTTCATTCGATCTGGGGTGGGGATGGGAGCAGCAGGGCCTGGGAATCAGTCCAATGAAA
  TAATGTCTGGTATGATGCCAAAGATGGATGCCGTGTCCGTGCTGCTCCTGTTTGTGACGG
  TGTGGCTATGGCTTTCCCTTAGGCTGTTGGAAGAAACCAGGCTGCTGTTACTGCCACTAC
  TGCTTCCATTGGTGGTTGGCAGAGAAGTACTGCCTCCTGCACTTAACTGATCAAGAAACA
  TCTGTGAGGAGGTATACGGGAAAAACCGAGACGAGTGTAGGAGGTCTTGATCATCTGAAA
  CTGCTGCTGCTGCAGCGGCAAGCAAGGAGGTGTTGTAATGCTGATTGTCTCCTGATAAGA
  AAGGAAAGAAATCTAATCCTTGTAAGTCGTAAGGCATGGGTGTCATGTGGAAAGGATGCC
  TATAGTCTTGGATGAGGGAGGTATTAATGTACCTTGTGTCTACAGCAGGAAGGCTTGGGG
  TGCGGNATACTGGAGATGCTTGATGTGGAAGACTTANAATGCCTTATTATTTANTGGTGA
  TGTGGGAGATAGGAAAGACAGGTCCCTCTGGCAAATAGCTCCTCCTTCTCTTCATCANAT
  GAACTGCCTATGGNTANGTCAATCACTTCTACTTTCCTGNTTTTATTTGAGGACCGGNTG
  GTGAGCACCCTACCTGATGCTCCATGGGGGAACTACAGCTTCATCGACTCCATTGGTAGA
  AGCAAAAACTNCCTGGACTCCCTTTTTGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016166
• ORF Size: 1956 bp
• Insert Size: 2320
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016166.1, NP_057250.1
• RefSeq Size: 2309
• RefSeq ORF: 1956
• Locus ID: 8554
• Domains: SAP, zf-MIZ
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (2) uses an alternate 5' structure which results in a distinct 5' UTR and 5' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.