TRPC4 (NM_016179) Human Untagged Clone

CAT#: SC114411

TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC4
• Synonyms: HTRP-4; HTRP4; TRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016179, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA
  GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG
  TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA
  AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG
  TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA
  CCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAATTCACT
  CCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTGGTTCAGA
  AAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGTTCAGATGT
  GGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCTCTCATTGCA
  CTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTGAGCAAGGTGG
  AAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAGGACCTACTGGA
  TCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGTCTCATAGAAGAA
  CAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCCCAGC
  CCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGC
  AGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCT
  CCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATT
  TGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
  ACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAG
  ATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCT
  TATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATC
  ATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTG
  CGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCC
  TGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATT
  GTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCA
  TTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGA
  CCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCAT
  CTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCAT
  GCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGC
  CTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTT
  GTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTG
  AGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAG
  ATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCG
  CTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCGAATGCCTCGAAGGAG
  TCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGGTAATAGCAAGGACAAGAAAAAGAATT
  TCAGCCTTTTTGATTTAACCACCCTGATTCATCCGAGATCAGCAGCAATTGCCTCTGAAAGACATAACAT
  AAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCGCCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACC
  GATATCAAAAACTTTGGGTTATTTCATAGACGATCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAA
  TCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGA
  ATCTCGAGGATTAGCTTCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGAC
  CATAGAGAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGA
  AGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTAT
  AGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTACGTGACCACAAGATTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016179
• Insert Size: 3330 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016179.2, NP_057263.1
• RefSeq Size: 3534 bp
• RefSeq ORF: 2934 bp
• Locus ID: 7223
• Cytogenetics: 13q13.3
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1, also known as alpha) encodes isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.