ARSB (NM_000046) Human Untagged Clone

CAT#: SC114442

ARSB (untagged)-Human arylsulfatase B (ARSB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARSB
• Synonyms: ASB; G4S; MPS6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114442 sequence for NM_000046 edited (data generated by NextGen Sequencing)
  ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC
  CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG
  GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC
  GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG
  CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT
  GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC
  TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC
  CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA
  GGATTTGATACCTACTTTGGATATCTCCTGGGTAGTGAAGATTATTATTCCCATGAACGC
  TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA
  GAAGTTGCAACAGGATATAAAAATATGTATTCAACAAACATATTCACCAAAAGGGCTATA
  GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT
  GTGCATGAGCCCCTTCAGGTCCCTGAGGAATACTTGAAGCCATATGACTTTATCCAAGAC
  AAGAACAGGCATCACTATGCAGGAATGGTGTCCCTTATGGATGAAGCAGTAGGAAATGTC
  ACTGCAGCTTTAAAAAGCAGTGGGCTCTGGAACAACACGGTGTTCATCTTTTCTACAGAT
  AACGGAGGGCAGACTTTGGCAGGGGGTAATAACTGGCCCCTTCGAGGAAGAAAATGGAGC
  CTGTGGGAAGGAGGCGTCCGAGGGGTGGGCTTTGTGGCAAGCCCCTTGCTGAAGCAGAAG
  GGCGTGAAGAACCGGGAGCTCATCCACATCTCTGACTGGCTGCCAACACTCATGAAGCTG
  GCCAGGGGACACACCAATGGCACAAAGCCTCTGGATGGCTTCGACGTGTGGAAAACCATC
  AGTGAAGGAAGCCCATCCCCCAGAATTGAGCTGCTGCATAATATTGACCCGAACTTCGTG
  GACTCTTCACCGTGTCCCAGGAACAGCATGGCTCCAGCAAAGGATGACTCTTCTCTTCCA
  GAATATTCAGCCTTTAACACATCTGTCCATGCTGCAATTAGACATGGAAATTGGAAACTC
  CTCACGGGCTACCCAGGCTGTGGTTACTGGTTCCCTCCACCGTCTCAATACAATGTTTCT
  GAGATACCCTCATCAGACCCACCAACCAAGACCCTCTGGCTCTTTGATATTGATCGGGAC
  CCTGAAGAAAGACATGACCTGTCCAGAGAATATCCTCACATCGTCACAAAGCTCCTGTCC
  CGCCTACAGTTCTACCATAAACACTCAGTCCCCGTGTACTTCCCTGCACAGGACCCCCGC
  TGTGATCCCAAGGCCACTGGGGTGTGGGGCCCTTGGATGTAG
  
  Clone variation with respect to NM_000046.3
  1072 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000046 unedited
  GTCAGCTTTTGTAATACGACTTACNTATAGGGCGGCCGCGATTCGGCACGAGGGACAAGG
  ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC
  CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG
  GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC
  GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG
  CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT
  GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC
  TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC
  CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA
  GGATTTGATACCTACTTTGGATATCTCCTGNGTAGTGAAGATTATTATTCCCATGAACGC
  TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA
  GAAGTTGCAACAGGATATAAAAATATGTATTCAACANACATATTCACCAAAAGGGCTATA
  GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT
  GTGCATGAGCCNCCTTCAGTCCCTNGAGAATACTTGAAGCCATATGACTTTATCCAAGAC
  AGAACAGGCATCACTATGCAGGAATGGTGTCCCTTTATGATGAAGCAGTAGGAAATGTCA
  CTGCAGCTTTTAAAAGCAGTGGGCTCTGGAACAACACGTGTTCATCTTTCTACAGATACN
  GAGGGCAGACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000046 unedited
  NGGGTACTCTATGNCCGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTGAGTGTTGCAGATTTTATCAGCTTCTTAAATGCATTAGGGGTTGAAATTA
  GACACCTCGGTGTGGTTTAAGAGCAAGAGAAGGGCCAAGTGAACCCAGGTTGGGATAACA
  AATGAGACAAGAGTCGTGAGAAAAGGCCTGAGGTCCAACTTCCAATTGAAAGGTTTTCTA
  GCCTCCCTGAAATCCTACATCCAAGGGCCCCACACCCCAGTGGCCTTGGGATCACAGCGG
  GGGTCCTGTGCAGGGAAGTACACGGGGACTGAGTGTTTATGGTAGAACTGTAGGCGGGAC
  AGGAGCTTTGTGACGATGTGAGGATATTCTCTGGACAGGTCATGTCTTTCTTCAGGGTCC
  CGATCAATATCAAAGAGCCAGAGGGTCTTGGTTGGTGGGTCTGATGAGGGTATCTCAGAA
  ACATTGTATTGAGACGGTGGAGGGAACCAGTAACCACAGCCTGGGTAGCCCGTGAGGAGT
  TTCCAATTTCCATGTCTAATTGCAGCATGGACAGATGTGTTAAAGGCTGAATATTCTGGA
  AGAGAAGAGTCATCCTTTGCTGGAGCCATGCTGTTCCTGGGACACGGTGAAGAGTCCACG
  AAGTTCGGGTCAATATTATGCAGCAGCTCAATTCTGGGGGATGGGCTTCCTTCACTGATG
  GTTTTCCACACGTCGAAGCCATCCAGAGGCTTTGTGCCATTGGTGTGTCCCCTGCCCAGC
  TTCATGAGTGTTGGCAGCCAGTCAGAGATGTGGATGAAGCTCCGGTTCTTTACGCCCTTT
  TGCTTTAGCAAGGGGCTTGCCACAAAGCCCACCCTTGGGAAGCCTTCCTTCCACAGCTTC
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000046
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000046.2, NP_000037.2
• RefSeq Size: 6089 bp
• RefSeq ORF: 1602 bp
• Locus ID: 411
• Cytogenetics: 5q14.1
• Domains: Sulfatase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: 'Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.