ARSB (NM_000046) Human Untagged Clone

CAT#: SC114442

ARSB (untagged)-Human arylsulfatase B (ARSB), transcript variant 1


  "NM_000046" in other vectors (6)

Reconstitution Protocol

USD 900.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ARSB
Synonyms ASB; G4S; MPS6
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC114442 sequence for NM_000046 edited (data generated by NextGen Sequencing)
ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC
CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG
GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC
GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG
CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT
GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC
TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC
CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA
GGATTTGATACCTACTTTGGATATCTCCTGGGTAGTGAAGATTATTATTCCCATGAACGC
TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA
GAAGTTGCAACAGGATATAAAAATATGTATTCAACAAACATATTCACCAAAAGGGCTATA
GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT
GTGCATGAGCCCCTTCAGGTCCCTGAGGAATACTTGAAGCCATATGACTTTATCCAAGAC
AAGAACAGGCATCACTATGCAGGAATGGTGTCCCTTATGGATGAAGCAGTAGGAAATGTC
ACTGCAGCTTTAAAAAGCAGTGGGCTCTGGAACAACACGGTGTTCATCTTTTCTACAGAT
AACGGAGGGCAGACTTTGGCAGGGGGTAATAACTGGCCCCTTCGAGGAAGAAAATGGAGC
CTGTGGGAAGGAGGCGTCCGAGGGGTGGGCTTTGTGGCAAGCCCCTTGCTGAAGCAGAAG
GGCGTGAAGAACCGGGAGCTCATCCACATCTCTGACTGGCTGCCAACACTCATGAAGCTG
GCCAGGGGACACACCAATGGCACAAAGCCTCTGGATGGCTTCGACGTGTGGAAAACCATC
AGTGAAGGAAGCCCATCCCCCAGAATTGAGCTGCTGCATAATATTGACCCGAACTTCGTG
GACTCTTCACCGTGTCCCAGGAACAGCATGGCTCCAGCAAAGGATGACTCTTCTCTTCCA
GAATATTCAGCCTTTAACACATCTGTCCATGCTGCAATTAGACATGGAAATTGGAAACTC
CTCACGGGCTACCCAGGCTGTGGTTACTGGTTCCCTCCACCGTCTCAATACAATGTTTCT
GAGATACCCTCATCAGACCCACCAACCAAGACCCTCTGGCTCTTTGATATTGATCGGGAC
CCTGAAGAAAGACATGACCTGTCCAGAGAATATCCTCACATCGTCACAAAGCTCCTGTCC
CGCCTACAGTTCTACCATAAACACTCAGTCCCCGTGTACTTCCCTGCACAGGACCCCCGC
TGTGATCCCAAGGCCACTGGGGTGTGGGGCCCTTGGATGTAG

Clone variation with respect to NM_000046.3
1072 g=>a
>OriGene 5' read for NM_000046 unedited
GTCAGCTTTTGTAATACGACTTACNTATAGGGCGGCCGCGATTCGGCACGAGGGACAAGG
ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC
CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG
GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC
GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG
CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT
GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC
TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC
CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA
GGATTTGATACCTACTTTGGATATCTCCTGNGTAGTGAAGATTATTATTCCCATGAACGC
TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA
GAAGTTGCAACAGGATATAAAAATATGTATTCAACANACATATTCACCAAAAGGGCTATA
GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT
GTGCATGAGCCNCCTTCAGTCCCTNGAGAATACTTGAAGCCATATGACTTTATCCAAGAC
AGAACAGGCATCACTATGCAGGAATGGTGTCCCTTTATGATGAAGCAGTAGGAAATGTCA
CTGCAGCTTTTAAAAGCAGTGGGCTCTGGAACAACACGTGTTCATCTTTCTACAGATACN
GAGGGCAGACTT
>OriGene 3' read for NM_000046 unedited
NGGGTACTCTATGNCCGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTGAGTGTTGCAGATTTTATCAGCTTCTTAAATGCATTAGGGGTTGAAATTA
GACACCTCGGTGTGGTTTAAGAGCAAGAGAAGGGCCAAGTGAACCCAGGTTGGGATAACA
AATGAGACAAGAGTCGTGAGAAAAGGCCTGAGGTCCAACTTCCAATTGAAAGGTTTTCTA
GCCTCCCTGAAATCCTACATCCAAGGGCCCCACACCCCAGTGGCCTTGGGATCACAGCGG
GGGTCCTGTGCAGGGAAGTACACGGGGACTGAGTGTTTATGGTAGAACTGTAGGCGGGAC
AGGAGCTTTGTGACGATGTGAGGATATTCTCTGGACAGGTCATGTCTTTCTTCAGGGTCC
CGATCAATATCAAAGAGCCAGAGGGTCTTGGTTGGTGGGTCTGATGAGGGTATCTCAGAA
ACATTGTATTGAGACGGTGGAGGGAACCAGTAACCACAGCCTGGGTAGCCCGTGAGGAGT
TTCCAATTTCCATGTCTAATTGCAGCATGGACAGATGTGTTAAAGGCTGAATATTCTGGA
AGAGAAGAGTCATCCTTTGCTGGAGCCATGCTGTTCCTGGGACACGGTGAAGAGTCCACG
AAGTTCGGGTCAATATTATGCAGCAGCTCAATTCTGGGGGATGGGCTTCCTTCACTGATG
GTTTTCCACACGTCGAAGCCATCCAGAGGCTTTGTGCCATTGGTGTGTCCCCTGCCCAGC
TTCATGAGTGTTGGCAGCCAGTCAGAGATGTGGATGAAGCTCCGGTTCTTTACGCCCTTT
TGCTTTAGCAAGGGGCTTGCCACAAAGCCCACCCTTGGGAAGCCTTCCTTCCACAGCTTC
A
Restriction Sites NotI-NotI     
ACCN NM_000046
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000046.2, NP_000037.2
RefSeq Size 6089 bp
RefSeq ORF 1602 bp
Locus ID 411
Cytogenetics 5q14.1
Domains Sulfatase
Protein Families Druggable Genome
Protein Pathways Glycosaminoglycan degradation, Lysosome, Metabolic pathways
Gene Summary 'Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]'
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.

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