MRPS33 (NM_016071) Human Untagged Clone

CAT#: SC114481

MRPS33 (untagged)-Human mitochondrial ribosomal protein S33 (MRPS33), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPS33
• Synonyms: CGI-139; MRP-S33; PTD003; S33mt
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114481 sequence for NM_016071 edited (data generated by NextGen Sequencing)
  ATGTCCTCCCTTTCAGAATATGCCTTCCGCATGTCTCGTCTCAGTGCCCGGCTATTTGGT
  GAAGTCACCAGGCCTACTAATTCCAAGTCTATGAAAGTGGTGAAACTGTTTAGTGAACTG
  CCCTTGGCCAAGAAGAAGGAGACTTATGATTGGTATCCAAATCACCACACTTACGCTGAA
  CTCATGCAGACGCTCCGATTTCTTGGACTCTACAGAGATGAGCATCAGGATTTTATGGAT
  GAGCAAAAACGACTAAAGAAGCTTCGTGGAAAGGAGAAACCAAAGAAAGGAGAAGGGAAA
  AGAGCAGCAAAAAGGAAATAG
  
  Clone variation with respect to NM_016071.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016071 unedited
  GGCTTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCACTGT
  AGCTTTGGGTGGTGGGCTGCAGATTAATTTTGTAACCACCTTAAGAAAAATACGGAACTC
  TAACTCCTTGCCACTCAAGAAATGTCCTCCCTTTCAGAATATGCCTTCCGCATGTCTCGT
  CTCAGTGCCCGGCTATTTGGTGAAGTCACCAGGCCTACTAATTCCAAGTCTATGAAAGTG
  GTGAAACTGTTTAGTGAACTGCCCTTGGCCAAGAAGAAGGAGACTTATGATTGGTATCCA
  AATCACCACACTTACGCTGAACTCATGCAGACGCTCCGATTTCTTGGACTCTACAGAGAT
  GAGCATCAGGATTTTATGGATGAGCAAAAACGACTAAAGAAGCTTCGTGGAAAGGAGAAA
  CCAAAGAAAGGAGAAGGGAAAAGAGCAGCAAAAAGGAAATAGTGTTGGTCCCTCAAGAGG
  GAGACTTTCTTCCTCAGTGGCGGAGAGAAGAAAGTGCATTTATTGTCTTTCCACATATTG
  GAGGAATGTCATCTTCCTAAATGAAGTTTATTTGGAGGAACACAGTCATCTCCTTGGTGA
  AATCTAATCCGGTTACATTGTGGCTGGTTTCTTGAACACATTCTAACTGTGCAAAATTAT
  CTTGGCCTTGGCCGTGTAATGTGAGGTTTACCTGATTCTCTAATGAAATAAATACCTAAG
  TTATTGTTTGATGATTTGTTTGGGGTGGGATGGGATGCTGGGGGAATGGAGGGGAAACCT
  CATTAAGTGAGCATCCTATAGTGTCCTCATTTCTCCCTTCTTGCTTACTGACTCACCCAG
  CCCTAAACTTCTTTCATCCTGGGCCTTTCAGCCACTGTCTCCACCACCAAGCTCACAAAC
  TTTGNNGNNGTGTCTCACCTACTCCTTNGAGAATCTATAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016071
• ORF Size: 321 bp
• Insert Size: 1610
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016071.2, NP_057155.1
• RefSeq Size: 727
• RefSeq ORF: 321
• Locus ID: 51650
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.