UBC6e (UBE2J1) (NM_016021) Human Untagged Clone

CAT#: SC114499

UBE2J1 (untagged)-Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2J1
• Synonyms: CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114499 sequence for NM_016021 edited (data generated by NextGen Sequencing)
  ATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTTAATGAAAGAAGCGGCA
  GAATTGAAAGATCCAACAGATCATTACCATGCGCAGCCTTTAGAGGATAACCTTTTTGAA
  TGGCACTTCACGGTTAGAGGGCCCCCAGACTCCGATTTTGATGGAGGAGTTTATCACGGG
  CGGATAGTACTGCCACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCT
  AATGGTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCATCCTGAA
  ACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCATCATTGGGTTTATGCCA
  ACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATTACACTCCTGAGGAAAGAAGAGCACTT
  GCCAAAAAATCACAAGATTTCTGTTGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTG
  TTGCCTTTAAAATCTGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCT
  AGGCAAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAGTCAGAC
  TTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACCTACAACATTCCAGGGT
  GCTACGGCCAGTACATCGTACGGAGTCCAGAATTCCTCAGCAGCATCCTTTCATCAACCT
  ACCCAACCTGTAGCTAAGAATACCTCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAG
  AGTCAGAGAAGGTTGTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAAC
  CACACTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTGGCAGCT
  CTTATATTCCGACGAATATATCTGGCAAACGAATACATATTTGACTTTGAGTTATAA
  
  Clone variation with respect to NM_016021.2
  685 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016021 unedited
  GTTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCCGAGCCGCGCTG
  AGACCCGGGCAGCGGCCGCGTGGAGAGGAGGTGGCAGCGGCCCGGGAGGCCGGAGCCAAG
  CCAGCGACCCACCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTTAAT
  GAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGCAGCCTTTAGAGGA
  TAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCCCCAGACTCCGATTTTGATGGAGG
  AGTTTATCACGGGCGGATAGTACTGCCACCAGAGTATCCCATGAAACCACCAAGCATTAT
  TCTCCTAACGGCTAATGGTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGG
  CCATCATCCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCATCAT
  TGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATTACACTCCTGAGGA
  AAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGTTGTGAAGGATGTGGCTCTGCCAT
  GAAGGATGTCCTGTTGCCTTTAAAATCTGGAAGCGATTCAAGCCAAGCTGACCAAGAAGC
  CAAAGAACTGGCTAGGCAAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTAT
  CTCTGAGTCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACCTAC
  AACATTCCAGGGTGCTACGGCCAGTACATCGTACGGAGTCCAGAATTCCTCAGCAGCATC
  CTTTCATCAACCTACCCAACCTGTAGCTAAGAATACCTNNCATGAGCCTCGACAGCGCCG
  GGCCCAGCAGCAGAGTCAGAGAGGTTGTCTACTTCCCAGATGTATCCAGGGNCACAGCCA
  GAGACACACCTGATCATGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016021 unedited
  GACGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAAAAGACTGAATTTATT
  AAAGTTTAAGACAATTCTGTATTATATAATAGTGTACAATATGTTCTTATTTTAGATGTA
  GAAATAAAGGAAAACTGGCTCTCAGATGAAAACAGAAATTGTGTCTGACAGTTAATAGAA
  CGAATGTCCCATAAACAGGAGTAAAAATTAAATTTGCCTTTAAGACGATTTTACAATTAT
  TTCTCTTGTCCATAATTTCACTTAGTTAAAACCACATAAGGACTTTATGAATACCCTCCC
  CTATACTATTCAAGATATACATATGGGCTAACTTTCGTGGACCGATCTATAACCTAAATA
  CCACTTCCAACATTGTTTGACTTGTAAAAAAATTAACTTACAAATAACACTTTTGGAGCA
  TAACCTATTTGAAAGCTTGGAACCACCTGTATTTAAAAATTAAATCTTCATTAACAACAC
  CCTCAAGAGTGCCCTCAGTGCTGCGAACTGAACATGCTGAAGGCAATGTTTTTCTGTGAT
  TATATGGTTTCACCAGCCATTATGATGATAATATTATCTACTAAAGTCTGAGGCGCTAGC
  TAACACTAAGTTATATATTGATTTTTCCACTGAAAATTCTTTCTTGAGCTCTGACTAAGA
  GGACAATTCTAAGTAAAGGGTTTTCTAACTTCTATACCTGTTACTGGGGCCCTGAGGTTT
  CCCATTAAAATAGGACCTGAATATTTCCATTTTATGCATTTTCCCGGAATAATAAAATTC
  TTCCGGTTTATAACGCTGGCCTTTATACCTCCCAAAGACATCCTTTCAAGAAAACCAACA
  TCCAATTCTCCAATCCCCCCTCTACTTCTGGAAATACCCCGCATTCTCTGGTTGGGCCCT
  TCTTCTTAAAACACTAGAAAACCGCCTCCTTCTCTTAACTTGCGGCATATTGCAAACAAA
  GGAGGGCTGGGAAACCAGTNTTAAATGCTACAAGCACACCTTCAACGAATACAGCGCCAA
  TTTATCTCTNT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016021
• ORF Size: 957 bp
• Insert Size: 2710
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016021.2, NP_057105.2
• RefSeq Size: 4360
• RefSeq ORF: 957
• Locus ID: 51465
• Domains: UBCc
• Protein Families: Transmembrane
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]