MRPL4 (NM_015956) Human Untagged Clone

CAT#: SC114544

MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL4
• Synonyms: CGI-28; L4mt
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114544 sequence for NM_015956 edited (data generated by NextGen Sequencing)
  ATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGC
  CTGAGTTCCCTGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGC
  GAGGGTCTCCCGGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGC
  CCAGTGCAGGCCTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCC
  GACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCT
  ATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTG
  CGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGC
  ATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCACAAGT
  TACTACTACATGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTC
  AAGCTGGCCCAGGACGACCTGCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCA
  CAGTACCTGACAGAGCTGGCGCACTACCGCCGCTGGGGGGACTCCGTACTCCTCGTGGAC
  TTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCACCTCTAGGCTTAAGACCTTC
  AACTTGATCCCGGCTGTTGGCCTAAATGTGCACAGCATGCTCAAGCACCAGACGCTGGTC
  CTGACGCTGCCCACCGTCGCCTTCCTGGAGGACAAGCTGCTCTGGCAGGACTCACGTTAC
  AGACCCCTCTACCCCTTCAGCCTGCCCTACAGCGACTTCCCCCGACCCCTACCCCACGCT
  ACCCAGGGCCCAGCGGCCACCCCGTACCACTGTTGA
  
  Clone variation with respect to NM_015956.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015956 unedited
  TATTTCCCCCGCCCGNTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGCGAGGCTCCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAG
  TTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCC
  CTGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTC
  CCGGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAG
  GCCTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCAC
  CCCGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAG
  AAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGGGCGGT
  GGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCT
  CCGCTCTGGCGAGGAGGANGTGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTAC
  ATGCTGNCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCC
  CCAGACGACCTGCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTG
  ACAGAGCTTGCGCACTACCGCCGCTGTGGGGACTTCGTACTCCTCGTGGCTTTATCCACC
  GAGAGATGCCCAGAGCATCGTGGAGGCCCCTCTATGCTTAAGACCTTCACTTGATCCCGG
  CTGTTGCCCTAATGTGCCCAGATGCTTCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015956 unedited
  NCGGTTTAGCTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTA
  TCCTCCTTGATTCAAAATACAATTTATTTTAAAAACAAAAAGAGGGAATGGATGCAGAAG
  CCCGTCTGGGCTCTCCGTGGAGTCGCCCCTTCCCAATGGCGATGGCCGCTCCCTGTGGCC
  TCAGCTTCCTCCGTGGAATGAAGGGGTCCAGGTGACACCTTCCTCGAAGGGTGGGCGTGG
  GCCTAAGGCTCCCAAGTCGGCCAGGGGCTCGGCCTGGCACAGAAGAGGTGCTTCACATCA
  ACAGTGGTACGGGGTGGCCGCTGGGCCCTGGGTAGCGTGGGGTAGGGGTCGGGGGAAGTC
  GCTGTAGGGCAGGCTGAAGGGGTAGAGGGGTCTGTAACGTGAGTCCTGCCAGAGCAGCTT
  GTCCTCCAGGAAGGCGACGGTGGGCAGCGTCAGGACCAGCGTCTGGTGCTTGAGCATGCT
  GTGCACATTTAGGCCAACAGCCGGGATCAAGTTGAAGGTCTTAAGCCTAGAGGTGGCCTC
  CACGATGCTCTGTGGCATCTCCTCGTGTGTTAAGTCCACGAGGAGTACGGAGTCCCCCCA
  GCGGCGGTAGTGCGCCAGCTCTGTCAGGTACTGTGGGTCTCCGGTGGGCAGCTCTAGGGA
  GTCCATGATGTGCAAGTCGTCCTGGGCCAGCTTGACCGTCAGTGCCCCTTTGAGACCCAG
  CGCCCGCACCTTCATGGGCAGCATGTAGTAATAACTTGTGGGGGCCCCGGGGCCATGGGG
  CACACCCTCTTCTTCCCAAACCGGAAAGCGGATGCTGCCATGCCCGGGCCCCCCCATGGC
  CTTTTTGCGGGCAAAGGTTTCGGGCCCCGCCCCCCCCTTGGTTCTTGTTTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015956
• ORF Size: 936 bp
• Insert Size: 1250
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_015956.2, NP_057040.2
• RefSeq Size: 1511
• RefSeq ORF: 936
• Locus ID: 51073
• Domains: Ribosomal_L4
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is the predominant transcript and it encodes the same isoform (a) as variant 2.