DBC1 (BRINP1) (NM_014618) Human Untagged Clone

CAT#: SC114955

DBC1 (untagged)-Human deleted in bladder cancer 1 (DBC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRINP1
• Synonyms: DBC1; DBCCR1; FAM5A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114955 sequence for NM_014618 edited (data generated by NextGen Sequencing)
  ATGAACTGGAGGTTTGTTGAGCTCCTCTACTTCCTGTTTATATGGGGCCGTATCTCAGTG
  CAGCCCTCCCACCAGGAACCAGCTGGGACAGACCAACATGTCTCCAAGGAATTTGATTGG
  CTCATTTCAGACAGGGGGCCTTTCCACCACTCCAGGAGCTACCTATCCTTTGTGGAAAGA
  CACCGTCAAGGATTTACAACCAGATATAAAATATACAGGGAGTTTGCCCGTTGGAAGGTG
  AGGAACACAGCCATCGAGAGGAGAGATCTGGTCCGCCATCCAGTGCCCCTCATGCCGGAG
  TTTCAAAGGAGCATCCGCCTGCTTGGCAGGAGACCTACCACTCAGCAGTTCATCGATACC
  ATCATCAAAAAGTACGGCACCCACCTGCTCATCTCAGCCACATTGGGAGGGGAGGAGGCT
  TTGACCATGTATATGGACAAAAGTCGCCTCGACAGGAAGTCAGGGAATGCCACTCAAAGT
  GTTGAAGCTCTGCACCAGCTCGCATCATCCTACTTTGTTGACCGTGATGGTACCATGAGG
  AGGCTTCATGAGATCCAGATATCAACTGGAGCAATCAAGGTCACAGAGACACGCACTGGG
  CCTCTGGGCTGTAACAGCTATGACAATCTGGACTCTGTGAGTTCCGTCCTTCTGCAAAGC
  ACGGAGAGCAAACTGCACCTTCAAGGTCTTCAGATAATCTTTCCTCAGTATCTGCAAGAG
  AAGTTTGTCCAGTCGGCCTTGAGCTATATCATGTGCAATGGGGAGGGGGAGTACCTGTGC
  CAGAACAGCCAGTGTCGCTGCCAATGTGCCGAGGAGTTTCCGCAGTGCAACTGCCCCATC
  ACGGACATCCAGATCATGGAGTACACGCTGGCCAACATGGCCAAGTCTTGGGCCGAAGCT
  TATAAGGACCTGGAGAATTCAGATGAGTTTAAATCATTTATGAAGCGCCTCCCCAGCAAC
  CACTTCCTGACCATCGGAAGCATCCATCAGCACTGGGGCAATGACTGGGACCTGCAGAAC
  CGCTACAAGCTCCTGCAGAGTGCCACGGAGGCACAGAGACAAAAGATCCAACGCACTGCC
  CGCAAGCTTTTCGGCCTCAGTGTACGCTGTCGCCACAATCCCAACCACCAGCTGCCTAGA
  GAGAGGACAATTCAGCAGTGGCTTGCAAGGGTCCAGTCACTCCTCTACTGTAATGAGAAT
  GGGTTTTGGGGAACCTTCCTGGAGAGCCAGCGGAGCTGCGTGTGCCACGGCAGCACCACG
  CTGTGCCAGCGCCCCATCCCCTGCGTGATAGGCGGGAACAACAGCTGCGCCATGTGCAGC
  CTGGCCAACATCTCCCTCTGCGGCTCCTGCAACAAGGGCTACAAGCTGTATCGAGGCCGC
  TGTGAACCACAGAACGTGGACTCGGAGCGGAGCGAGCAGTTCATCAGCTTTGAGACTGAC
  CTGGACTTCCAGGACCTGGAGCTGAAGTACCTGCTGCAGAAGATGGACTCACGCCTCTAC
  GTCCACACCACCTTCATCAGCAACGAGATCCGCCTCGACACCTTCTTTGACCCTCGGTGG
  CGCAAGCGCATGTCCCTCACTCTCAAGAGCAACAAGAACCGCATGGACTTCATCCACATG
  GTGATCGGCATGTCCATGCGCATCTGCCAGATGCGCAACAGCAGCCTGGACCCCATGTTC
  TTTGTCTATGTCAACCCCTTTAGCGGGAGCCATTCGGAGGGCTGGAACATGCCCTTCGGG
  GAATTTGGCTACCCACGCTGGGAGAAGATCCGTCTCCAAAACAGCCAGTGCTACAACTGG
  ACTCTTTTGCTGGGCAATCGGTGGAAAACATTTTTCGAGACGGTCCACATCTACCTACGT
  AGTCGGACTCGGCTACCTACCCTACTGCGAAATGAGACTGGCCAGGGCCCCGTGGACCTG
  TCGGATCCCTCCAAGAGGCAGTTCTACATCAAGATCTCAGACGTGCAGGTGTTTGGGTAT
  AGCCTGAGGTTCAACGCCGACCTCCTGCGCAGTGCAGTGCAGCAGGTCAACCAGTCCTAC
  ACACAGGGCGGCCAGTTCTATTCCTCTTCGTCAGTGATGCTCCTCTTGTTGGATATTCGG
  GACCGAATTAATCGCCTGGTCCCTCCTGTGGCCCCGGGGAAACCCCAGCTGGACTTGTTC
  TCCTGTATGCTGAAACACCGCCTGAAACTGACCAACAGCGAGATCATCAGGGTGAACCAC
  GCCTTGGACCTGTACAACACGGAGATCCTCAAACAGTCGGACCAGATGACAGCCAAACTC
  TGCTAA
  
  Clone variation with respect to NM_014618.2
  2120 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014618 unedited
  ATTTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCTCGGGACCAGC
  GAAGGGGACTTTACGGCTGAGTATGAGCCAGGCTGCTAGGAGCCAGGTACCCCCACGCCT
  GCAGTCCCCGCGCCGTGCCCGGAATGCGAGCTGGACGCAGGGCTCTCCCAAGTTCCCACC
  GAGCCGAATAAAAAGCGTCCGCCCGCAGCTCTCCGCCAAAGACGGACATTGACTCCAGGA
  CTCCACAGAATGGAGCAAGAATGAGAGGAAAAAGGCCGGCAGAAAAGCATGAACTGGAGG
  TTTGTTGAGCTCCTCTACTTCCTGTTTATATGGGGCCGTATCTCAGTGCAGCCCTCCCAC
  CAGGAACCAGCTGGGACAGACCAACATGTCTCCAAGGAATTTGATTGGCTCATTTCAGAC
  AGGGGGCCTTTCCACCACTCCAGGAGCTACCTATCCTTTGTGGAAAGACACCGTCAAGGA
  TTTACAACCAGATATAAAATATACAGGGAGTTTGCCCGTTGGAAGGTGAGGAACACAGCC
  ATCGAGAGGAGAGATCTGGTCCGCCATCCAGTGCCCCTCATGCCGGAGTTTCAAAGGAGC
  ATCCGCCTGCTTGGCAGGAGACCTACCACTCAGCAGTTCATCGATACCATCATCAAAAAA
  GTACGGCACCCACCTGCTCATCTCAGCCACATTGGGAGGGGAGGAGGCTNTGACCATGTA
  TATGGACAAAAGTCGCCTCGACAGGAAGTCAGGGAATGCCACTCAAAGTGGTGAAGCTCT
  GCACCAGCTCGCATCATCCTACTTTGNTGACCGTGATGGTACCATGAGGAGCTCATGAGA
  TCCAGATATCNACTGGGAGCATCAGGTCACAGAGACCGCACTGGGCCTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014618 unedited
  TGGCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTCATTTTGTTCT
  GTTGTGTGTGTACAACAACAGGGAAAAGTCCATGGCAAGGAGTCCCGGGTTAGCAGAGTT
  TGGCTGTCATCTGGTCCGACTGTTTGAGGATCTCCGTGTTGTACAGGTCCAAGGCGTGGT
  TCACCCTGATGATCTCGCTGTTGGTCACTTTCAGGCGGTGTTTCACCATACAGGAGAACA
  AGTCCAGCTGGGGTTTCCCCGGGGCCACAGGAGGGACCAGGCGATTAATTCGGTCCCGAA
  TATCCAACAAGAGGAGCATCACTGACCAATAGGAATACAACTGGCCGCCCTGTGTGTAGG
  ACTGGTTGACCTGCTGCACTGCACTGCGCAGGAGGTCGGCGTTGAACCTCAGGCTATACC
  CAAACACCTGCACGTCTGAGATCTTGATGTAGAACTGCCTCTTGGAGGGATCCGACAGGT
  CCACGGGGCCCTGGCCAGTCTCATTTCGCAGTAGGGTAGGTACCCCAGTCCGACTACGTA
  CGCAGATGTGGACCGCCTTCGAAAAATGTTTTCCACCGATTGCCCAGCAAAAGAGTCCAG
  TTGTACCACTGGCTGTCTTGGACACGGATCCTTCTCCCAGCGTGGGTAGCCAACATTCCC
  GAACGGCATGTTCCCAGCCCTCCCAATGCCTTCCGTTAAAGGGGCTGACATAGACAAACA
  CATTGGGCCCAGCCTGCTGTTGCCCATCTGCCAAATGCCCATGAACCTCCCGACCACATG
  TCGGATGAAACCCTGCCGCCCCCTGCTCCCCTTTAAAATGAGGGACCTTGCCCTTGCCCC
  CCCGATGGCCAACTTACGTCGCCCAGCGCGATCCCCTCTTTTCATCACGTGCTTTCGCCC
  CCACAGCCTCTATCCTCCCTTCTTAAAAGGTCCTTCATCTCCAGGTCCTGGAACCCCAGA
  TATATCCAAACTTAACACTGCTCCTCCCCTCCACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014618
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014618.1, NP_055433.1
• RefSeq Size: 3158 bp
• RefSeq ORF: 2286 bp
• Locus ID: 1620
• Cytogenetics: 9q33.1
• Domains: MACPF
• Gene Summary: 'This gene is located within a chromosomal region that shows loss of heterozygosity in some bladder cancers. It contains a 5' CpG island that may be a frequent target of hypermethylation, and it may undergo hypermethylation-based silencing in some bladder cancers. [provided by RefSeq, Jul 2008]'